A xylogalacturonan epitope is specifically associated with plant cell detachment.

W.G.T. Willats; L. McCartney; C.G. Steele-King; S.E. Marcus; A.J. Mort; M.M.H. Huisman; G.J.W.M. van Alebeek; H.A. Schols; A.G.J. Voragen; A. le Goff; E. Bonnin; J.F. Thibault; J.P. Knox

doi:10.1007/s00425-003-1147-8

A xylogalacturonan epitope is specifically associated with plant cell detachment.

W.G.T. Willats, L. McCartney, C.G. Steele-King, S.E. Marcus, A.J. Mort, M.M.H. Huisman, G.J.W.M. van Alebeek, H.A. Schols, A.G.J. Voragen, A. le Goff, E. Bonnin, J.F. Thibault, J.P. Knox

Research output: Contribution to journal › Article › Academic › peer-review

103 Citations (Scopus)

Abstract

A monoclonal antibody (LM8) was generated with specificity for xyloglacturonan (XGA) isolated from pea (Pisum sativum L.) testae. Characterization of the LM8 epitope indicates that it is a region of XGA that is highly substituted with xylose. Immunocytochemical analysis indicates that this epitope is restricted to loosely attached inner parenchyma cells at the inner face of the pea testa and does not occur in other cells of the testa. Elsewhere in the pea seedling, the LM8 epitope was found only in association with root cap cell development at the root apex. Furthermore, the LM8 epitope is specifically associated with root cap cells in a range of angiosperm species. In embryogenic carrot suspension cell cultures the epitope is abundant at the surface of cell walls of loosely attached cells in both induced and non-induced cultures. The LM8 epitope is the first cell wall epitope to be identified that is specifically associated with a plant cell separation process that results in complete cell detachment.

Original language	English
Pages (from-to)	673-681
Journal	Planta
Volume	218
Issue number	4
DOIs	https://doi.org/10.1007/s00425-003-1147-8
Publication status	Published - 2004

Keywords

pectic polysaccharides
spatial regulation
hairy regions
pea hulls
walls
homogalacturonan
tomato
cotyledons
separation
pericarp

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Willats, W. G. T., McCartney, L., Steele-King, C. G., Marcus, S. E., Mort, A. J., Huisman, M. M. H., van Alebeek, G. J. W. M., Schols, H. A., Voragen, A. G. J., le Goff, A., Bonnin, E., Thibault, J. F., & Knox, J. P. (2004). A xylogalacturonan epitope is specifically associated with plant cell detachment. Planta, 218(4), 673-681. https://doi.org/10.1007/s00425-003-1147-8

@article{c5b3120876ee4202b6bb44d3c648386e,

title = "A xylogalacturonan epitope is specifically associated with plant cell detachment.",

abstract = "A monoclonal antibody (LM8) was generated with specificity for xyloglacturonan (XGA) isolated from pea (Pisum sativum L.) testae. Characterization of the LM8 epitope indicates that it is a region of XGA that is highly substituted with xylose. Immunocytochemical analysis indicates that this epitope is restricted to loosely attached inner parenchyma cells at the inner face of the pea testa and does not occur in other cells of the testa. Elsewhere in the pea seedling, the LM8 epitope was found only in association with root cap cell development at the root apex. Furthermore, the LM8 epitope is specifically associated with root cap cells in a range of angiosperm species. In embryogenic carrot suspension cell cultures the epitope is abundant at the surface of cell walls of loosely attached cells in both induced and non-induced cultures. The LM8 epitope is the first cell wall epitope to be identified that is specifically associated with a plant cell separation process that results in complete cell detachment.",

keywords = "pectic polysaccharides, spatial regulation, hairy regions, pea hulls, walls, homogalacturonan, tomato, cotyledons, separation, pericarp",

author = "W.G.T. Willats and L. McCartney and C.G. Steele-King and S.E. Marcus and A.J. Mort and M.M.H. Huisman and {van Alebeek}, G.J.W.M. and H.A. Schols and A.G.J. Voragen and {le Goff}, A. and E. Bonnin and J.F. Thibault and J.P. Knox",

year = "2004",

doi = "10.1007/s00425-003-1147-8",

language = "English",

volume = "218",

pages = "673--681",

journal = "Planta",

issn = "0032-0935",

publisher = "Springer",

number = "4",

}

TY - JOUR

T1 - A xylogalacturonan epitope is specifically associated with plant cell detachment.

AU - Willats, W.G.T.

AU - McCartney, L.

AU - Steele-King, C.G.

AU - Marcus, S.E.

AU - Mort, A.J.

AU - Huisman, M.M.H.

AU - van Alebeek, G.J.W.M.

AU - Schols, H.A.

AU - Voragen, A.G.J.

AU - le Goff, A.

AU - Bonnin, E.

AU - Thibault, J.F.

AU - Knox, J.P.

PY - 2004

Y1 - 2004

N2 - A monoclonal antibody (LM8) was generated with specificity for xyloglacturonan (XGA) isolated from pea (Pisum sativum L.) testae. Characterization of the LM8 epitope indicates that it is a region of XGA that is highly substituted with xylose. Immunocytochemical analysis indicates that this epitope is restricted to loosely attached inner parenchyma cells at the inner face of the pea testa and does not occur in other cells of the testa. Elsewhere in the pea seedling, the LM8 epitope was found only in association with root cap cell development at the root apex. Furthermore, the LM8 epitope is specifically associated with root cap cells in a range of angiosperm species. In embryogenic carrot suspension cell cultures the epitope is abundant at the surface of cell walls of loosely attached cells in both induced and non-induced cultures. The LM8 epitope is the first cell wall epitope to be identified that is specifically associated with a plant cell separation process that results in complete cell detachment.

AB - A monoclonal antibody (LM8) was generated with specificity for xyloglacturonan (XGA) isolated from pea (Pisum sativum L.) testae. Characterization of the LM8 epitope indicates that it is a region of XGA that is highly substituted with xylose. Immunocytochemical analysis indicates that this epitope is restricted to loosely attached inner parenchyma cells at the inner face of the pea testa and does not occur in other cells of the testa. Elsewhere in the pea seedling, the LM8 epitope was found only in association with root cap cell development at the root apex. Furthermore, the LM8 epitope is specifically associated with root cap cells in a range of angiosperm species. In embryogenic carrot suspension cell cultures the epitope is abundant at the surface of cell walls of loosely attached cells in both induced and non-induced cultures. The LM8 epitope is the first cell wall epitope to be identified that is specifically associated with a plant cell separation process that results in complete cell detachment.

KW - pectic polysaccharides

KW - spatial regulation

KW - hairy regions

KW - pea hulls

KW - walls

KW - homogalacturonan

KW - tomato

KW - cotyledons

KW - separation

KW - pericarp

U2 - 10.1007/s00425-003-1147-8

DO - 10.1007/s00425-003-1147-8

M3 - Article

SN - 0032-0935

VL - 218

SP - 673

EP - 681

JO - Planta

JF - Planta

IS - 4

ER -

A xylogalacturonan epitope is specifically associated with plant cell detachment.

Abstract

Keywords

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