A validated method for the quantification of enterodiol and enterolactone in plasma using isotope dilution liquid chromatography with tandem mass spectrometry

A. Kuijsten; M.N.C.P. Buijsman; I.C.W. Arts; P.P.J. Mulder; P.C.H. Hollman

doi:10.1016/j.jchromb.2005.06.004

A validated method for the quantification of enterodiol and enterolactone in plasma using isotope dilution liquid chromatography with tandem mass spectrometry

A. Kuijsten
, M.N.C.P. Buijsman
, I.C.W. Arts
, P.P.J. Mulder
, P.C.H. Hollman

Research output: Contribution to journal › Article › Academic › peer-review

17 Citations (Scopus)

Abstract

Enterolactone and enterodiol are phytoestrogens with structural similarity to endogenous estrogens. Because of their biological activities, they may affect the development of several diseases. To quantify enterodiol and enterolactone in plasma, we developed and validated a liquid chromatography-tandem mass spectrometry method with electrospray ionization using 13C 3 labeled isotopes. The method consists of a simple enzymatic hydrolysis and ether extraction followed by a rapid LC separation (run-time of 11 min). Detection limits as low as 0.15 nM for enterodiol and 0.55 nM for enterolactone were achieved. The within-run R.S.D. ranges from 3 to 6% and the between-run R.S.D. ranges from 10 to 14% for both enterolignans. This method allows simple, rapid, and sensitive quantification, and is suitable for measuring large numbers of samples

Original language	English
Pages (from-to)	178-184
Journal	Journal of Chromatography. B, Analytical technologies in the biomedical and life sciences
Volume	822
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jchromb.2005.06.004
Publication status	Published - 2005

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

time-resolved fluoroimmunoassay
binding globulin shbg
breast-cancer cells
serum concentrations
phytoestrogens
lignans
risk
secoisolariciresinol
foods
isoflavonoids

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10.1016/j.jchromb.2005.06.004

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Kuijsten, A., Buijsman, M. N. C. P., Arts, I. C. W., Mulder, P. P. J., & Hollman, P. C. H. (2005). A validated method for the quantification of enterodiol and enterolactone in plasma using isotope dilution liquid chromatography with tandem mass spectrometry. Journal of Chromatography. B, Analytical technologies in the biomedical and life sciences, 822(1-2), 178-184. https://doi.org/10.1016/j.jchromb.2005.06.004

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title = "A validated method for the quantification of enterodiol and enterolactone in plasma using isotope dilution liquid chromatography with tandem mass spectrometry",

abstract = "Enterolactone and enterodiol are phytoestrogens with structural similarity to endogenous estrogens. Because of their biological activities, they may affect the development of several diseases. To quantify enterodiol and enterolactone in plasma, we developed and validated a liquid chromatography-tandem mass spectrometry method with electrospray ionization using 13C 3 labeled isotopes. The method consists of a simple enzymatic hydrolysis and ether extraction followed by a rapid LC separation (run-time of 11 min). Detection limits as low as 0.15 nM for enterodiol and 0.55 nM for enterolactone were achieved. The within-run R.S.D. ranges from 3 to 6\% and the between-run R.S.D. ranges from 10 to 14\% for both enterolignans. This method allows simple, rapid, and sensitive quantification, and is suitable for measuring large numbers of samples",

keywords = "time-resolved fluoroimmunoassay, binding globulin shbg, breast-cancer cells, serum concentrations, phytoestrogens, lignans, risk, secoisolariciresinol, foods, isoflavonoids",

author = "A. Kuijsten and M.N.C.P. Buijsman and I.C.W. Arts and P.P.J. Mulder and P.C.H. Hollman",

year = "2005",

doi = "10.1016/j.jchromb.2005.06.004",

language = "English",

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journal = "Journal of Chromatography. B, Analytical technologies in the biomedical and life sciences",

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Kuijsten, A , Buijsman, MNCP, Arts, ICW, Mulder, PPJ & Hollman, PCH 2005, 'A validated method for the quantification of enterodiol and enterolactone in plasma using isotope dilution liquid chromatography with tandem mass spectrometry', Journal of Chromatography. B, Analytical technologies in the biomedical and life sciences, vol. 822, no. 1-2, pp. 178-184. https://doi.org/10.1016/j.jchromb.2005.06.004

A validated method for the quantification of enterodiol and enterolactone in plasma using isotope dilution liquid chromatography with tandem mass spectrometry. / Kuijsten, A.; Buijsman, M.N.C.P.; Arts, I.C.W. et al.
In: Journal of Chromatography. B, Analytical technologies in the biomedical and life sciences, Vol. 822, No. 1-2, 2005, p. 178-184.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - A validated method for the quantification of enterodiol and enterolactone in plasma using isotope dilution liquid chromatography with tandem mass spectrometry

AU - Kuijsten, A.

AU - Buijsman, M.N.C.P.

AU - Arts, I.C.W.

AU - Mulder, P.P.J.

AU - Hollman, P.C.H.

PY - 2005

Y1 - 2005

N2 - Enterolactone and enterodiol are phytoestrogens with structural similarity to endogenous estrogens. Because of their biological activities, they may affect the development of several diseases. To quantify enterodiol and enterolactone in plasma, we developed and validated a liquid chromatography-tandem mass spectrometry method with electrospray ionization using 13C 3 labeled isotopes. The method consists of a simple enzymatic hydrolysis and ether extraction followed by a rapid LC separation (run-time of 11 min). Detection limits as low as 0.15 nM for enterodiol and 0.55 nM for enterolactone were achieved. The within-run R.S.D. ranges from 3 to 6% and the between-run R.S.D. ranges from 10 to 14% for both enterolignans. This method allows simple, rapid, and sensitive quantification, and is suitable for measuring large numbers of samples

AB - Enterolactone and enterodiol are phytoestrogens with structural similarity to endogenous estrogens. Because of their biological activities, they may affect the development of several diseases. To quantify enterodiol and enterolactone in plasma, we developed and validated a liquid chromatography-tandem mass spectrometry method with electrospray ionization using 13C 3 labeled isotopes. The method consists of a simple enzymatic hydrolysis and ether extraction followed by a rapid LC separation (run-time of 11 min). Detection limits as low as 0.15 nM for enterodiol and 0.55 nM for enterolactone were achieved. The within-run R.S.D. ranges from 3 to 6% and the between-run R.S.D. ranges from 10 to 14% for both enterolignans. This method allows simple, rapid, and sensitive quantification, and is suitable for measuring large numbers of samples

KW - time-resolved fluoroimmunoassay

KW - binding globulin shbg

KW - breast-cancer cells

KW - serum concentrations

KW - phytoestrogens

KW - lignans

KW - risk

KW - secoisolariciresinol

KW - foods

KW - isoflavonoids

U2 - 10.1016/j.jchromb.2005.06.004

DO - 10.1016/j.jchromb.2005.06.004

M3 - Article

SN - 1570-0232

VL - 822

SP - 178

EP - 184

JO - Journal of Chromatography. B, Analytical technologies in the biomedical and life sciences

JF - Journal of Chromatography. B, Analytical technologies in the biomedical and life sciences

IS - 1-2

ER -

Kuijsten A , Buijsman MNCP, Arts ICW, Mulder PPJ, Hollman PCH. A validated method for the quantification of enterodiol and enterolactone in plasma using isotope dilution liquid chromatography with tandem mass spectrometry. Journal of Chromatography. B, Analytical technologies in the biomedical and life sciences. 2005;822(1-2):178-184. doi: 10.1016/j.jchromb.2005.06.004

A validated method for the quantification of enterodiol and enterolactone in plasma using isotope dilution liquid chromatography with tandem mass spectrometry

Abstract

UN SDGs

Keywords

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