A validated method for the quantification of enterodiol and enterolactone in plasma using isotope dilution liquid chromatography with tandem mass spectrometry

A. Kuijsten, M.N.C.P. Buijsman, I.C.W. Arts, P.P.J. Mulder, P.C.H. Hollman

Research output: Contribution to journalArticleAcademicpeer-review

12 Citations (Scopus)

Abstract

Enterolactone and enterodiol are phytoestrogens with structural similarity to endogenous estrogens. Because of their biological activities, they may affect the development of several diseases. To quantify enterodiol and enterolactone in plasma, we developed and validated a liquid chromatography-tandem mass spectrometry method with electrospray ionization using 13C 3 labeled isotopes. The method consists of a simple enzymatic hydrolysis and ether extraction followed by a rapid LC separation (run-time of 11 min). Detection limits as low as 0.15 nM for enterodiol and 0.55 nM for enterolactone were achieved. The within-run R.S.D. ranges from 3 to 6% and the between-run R.S.D. ranges from 10 to 14% for both enterolignans. This method allows simple, rapid, and sensitive quantification, and is suitable for measuring large numbers of samples
Original languageEnglish
Pages (from-to)178-184
JournalJournal of Chromatography. B, Analytical technologies in the biomedical and life sciences
Volume822
Issue number1-2
DOIs
Publication statusPublished - 2005

Keywords

  • time-resolved fluoroimmunoassay
  • binding globulin shbg
  • breast-cancer cells
  • serum concentrations
  • phytoestrogens
  • lignans
  • risk
  • secoisolariciresinol
  • foods
  • isoflavonoids

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