A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex

K. O'Donnell, C. Gueidan, S. Sink, P.R. Johnston, P.W. Crous, A. Glenn, R. Riley, N.C. Zitomer, P. Colyer, C. Waalwijk, T. van der Lee, A. Moretti, S. Kang, H.S. Kim, D.M. Geiser, J.H. Juba, R.P. Baayen, M.G. Cromey, S. Bithel, D.A. SuttonK. Skovgaard, R. Ploetz, H.C. Kistler, M. Elliot, M. Davis, B.A.J. Sarver

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Abstract

We constructed a two-locus database, comprising partial translation elongation factor (EF-1a) gene sequences and nearly full-length sequences of the nuclear ribosomal intergenic spacer region (IGS rDNA) for 850 isolates spanning the phylogenetic breadth of the Fusarium oxysporum species complex (FOSC). Of the 850 isolates typed, 101 EF-1a, 203 IGS rDNA, and 256 two-locus sequence types (STs) were differentiated. Analysis of the combined dataset suggests that two-thirds of the STs might be associated with a single host plant. This analysis also revealed that the 26 STs associated with human mycoses were genetically diverse, including several which appear to be nosocomial in origin. A congruence analysis, comparing partial EF-1a and IGS rDNA bootstrap consensus, identified a significant number of conflicting relationships dispersed throughout the bipartitions, suggesting that some of the IGS rDNA sequences may be non-orthologous. We also evaluated enniatin, fumonisin and moniliformin mycotoxin production in vitro within a phylogenetic framework.
Original languageEnglish
Pages (from-to)936-948
JournalFungal Genetics and Biology
Volume46
Issue number12
DOIs
Publication statusPublished - 2009

Keywords

  • vegetative compatibility groups
  • f-sp cubense
  • fragment-length-polymorphisms
  • intergenic spacer region
  • genetic diversity
  • ribosomal dna
  • fungus fusarium
  • discontinuous distribution
  • phylogenetic diversity
  • nectria-haematococca

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    O'Donnell, K., Gueidan, C., Sink, S., Johnston, P. R., Crous, P. W., Glenn, A., Riley, R., Zitomer, N. C., Colyer, P., Waalwijk, C., van der Lee, T., Moretti, A., Kang, S., Kim, H. S., Geiser, D. M., Juba, J. H., Baayen, R. P., Cromey, M. G., Bithel, S., ... Sarver, B. A. J. (2009). A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex. Fungal Genetics and Biology, 46(12), 936-948. https://doi.org/10.1016/j.fgb.2009.08.006