A thermophile under pressure: Transcriptional analysis of the response of Caldicellulosiruptor saccharolyticus to different H2 partial pressures

A.A.M. Bielen, M.R.A. Verhaart, A.L. Vanfossen, S.E. Blumer-Schuette, A.J.M. Stams, J. van der Oost, R.M. Kelly, S.W.M. Kengen

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

Increased hydrogen (H2) levels are known to inhibit H2 formation in Caldicellulosiruptor saccharolyticus. To investigate this organism's strategy for dealing with elevated H2 levels the effect of the hydrogen partial pressure (PH2) on fermentation performance was studied by growing cultures under high and low PH2 in a glucose limited chemostat setup. Transcriptome analysis revealed the upregulation of genes involved in the disposal of reducing equivalents under high PH2, like lactate dehydrogenase and alcohol dehydrogenase as well as the NADH-dependent and ferredoxin-dependent hydrogenases. These findings are in line with the observed shift in fermentation profiles from acetate production to the production of acetate, lactate and ethanol under high PH2. Moreover, differential transcription was observed for genes involved in carbon metabolism, fatty acid biosynthesis and several transport systems. In addition, presented transcription data provide evidence for the involvement of the redox sensing Rex protein in gene regulation under high PH2 cultivation conditions
Original languageEnglish
Pages (from-to)1837-1849
JournalInternational Journal of Hydrogen Energy
Volume38
Issue number4
DOIs
Publication statusPublished - 2013

Keywords

  • rex-family repressor
  • hydrogen-production
  • extreme thermophiles
  • thermoanaerobacter-ethanolicus
  • hyperthermophilic archaeon
  • ferredoxin oxidoreductase
  • cellulolytic bacterium
  • thermotoga-neapolitana
  • alcohol dehydrogenases
  • pyrococcus-furiosus

Fingerprint Dive into the research topics of 'A thermophile under pressure: Transcriptional analysis of the response of Caldicellulosiruptor saccharolyticus to different H2 partial pressures'. Together they form a unique fingerprint.

Cite this