A simple and rapid cultural method for detection of Enterobacter sakazakii in environmental samples

O. Guillaume-Gentil, V. Sonnard, M.C. Kandhai, J. Marugg, H. Joosten

Research output: Contribution to journalArticleAcademicpeer-review

58 Citations (Scopus)

Abstract

A method was developed to detect and identify Enterobacter sakazakii in environmental samples. The method is based on selective enrichment at 45 ± 0.5°C in lauryl sulfate tryptose broth supplemented with 0.5 M NaCl and 10 mg/liter vancomycin (mLST) for 22 to 24 h followed by streaking on tryptone soy agar with bile salts. When exposed to light during incubation at 37°C, E. sakazakii produces yellow colonies within 24 h; identification was confirmed by testing for -glucosidase activity and by using API 20E strips. All of the E. sakazakii strains tested (n = 99) were able to grow in mLST at 45 ± 0.58°C, whereas 35 of 39 strains of potential competitors, all belonging to the Enterobacteriaceae, were suppressed. A survey was carried out with 192 environmental samples from four different milk powder factories. Using this new protocol, E. sakazakii was isolated from almost 40% of the samples, whereas the reference procedure (enrichment in buffered peptone water, isolation on violet red bile glucose agar, and biochemical identification of randomly chosen colonies) only yielded 26% positive results. This selective method can be very useful for the rapid and reliable detection of E. sakazakii in environmental samples
Original languageEnglish
Pages (from-to)64-69
JournalJournal of Food Protection
Volume68
Issue number1
Publication statusPublished - 2005

Fingerprint

Cronobacter sakazakii
Agar
sampling
agar
Glucosidases
Peptones
glucosidases
vancomycin
bile salts
dried milk
peptones
Enterobacteriaceae
Vancomycin
bile
Bile Acids and Salts
factories
Bile
Powders
sulfates
Milk

Keywords

  • neonatal meningitis
  • infant formula
  • powdered milk
  • infections
  • system
  • food

Cite this

Guillaume-Gentil, O., Sonnard, V., Kandhai, M. C., Marugg, J., & Joosten, H. (2005). A simple and rapid cultural method for detection of Enterobacter sakazakii in environmental samples. Journal of Food Protection, 68(1), 64-69.
Guillaume-Gentil, O. ; Sonnard, V. ; Kandhai, M.C. ; Marugg, J. ; Joosten, H. / A simple and rapid cultural method for detection of Enterobacter sakazakii in environmental samples. In: Journal of Food Protection. 2005 ; Vol. 68, No. 1. pp. 64-69.
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Guillaume-Gentil, O, Sonnard, V, Kandhai, MC, Marugg, J & Joosten, H 2005, 'A simple and rapid cultural method for detection of Enterobacter sakazakii in environmental samples', Journal of Food Protection, vol. 68, no. 1, pp. 64-69.

A simple and rapid cultural method for detection of Enterobacter sakazakii in environmental samples. / Guillaume-Gentil, O.; Sonnard, V.; Kandhai, M.C.; Marugg, J.; Joosten, H.

In: Journal of Food Protection, Vol. 68, No. 1, 2005, p. 64-69.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - A simple and rapid cultural method for detection of Enterobacter sakazakii in environmental samples

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AU - Sonnard, V.

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AU - Joosten, H.

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AB - A method was developed to detect and identify Enterobacter sakazakii in environmental samples. The method is based on selective enrichment at 45 ± 0.5°C in lauryl sulfate tryptose broth supplemented with 0.5 M NaCl and 10 mg/liter vancomycin (mLST) for 22 to 24 h followed by streaking on tryptone soy agar with bile salts. When exposed to light during incubation at 37°C, E. sakazakii produces yellow colonies within 24 h; identification was confirmed by testing for -glucosidase activity and by using API 20E strips. All of the E. sakazakii strains tested (n = 99) were able to grow in mLST at 45 ± 0.58°C, whereas 35 of 39 strains of potential competitors, all belonging to the Enterobacteriaceae, were suppressed. A survey was carried out with 192 environmental samples from four different milk powder factories. Using this new protocol, E. sakazakii was isolated from almost 40% of the samples, whereas the reference procedure (enrichment in buffered peptone water, isolation on violet red bile glucose agar, and biochemical identification of randomly chosen colonies) only yielded 26% positive results. This selective method can be very useful for the rapid and reliable detection of E. sakazakii in environmental samples

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Guillaume-Gentil O, Sonnard V, Kandhai MC, Marugg J, Joosten H. A simple and rapid cultural method for detection of Enterobacter sakazakii in environmental samples. Journal of Food Protection. 2005;68(1):64-69.