A simple amino acid dose-response technique to quantify amino acid requirements of individual meal-fed pigs

E. Kampman-van de Hoek, W.J.J. Gerrits, C.M.C. van der Peet-Schwering, A.J.M. Jansman, J.J.G.C. van den Borne

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Two experiments were conducted to develop a simplified dose-response technique to estimate the Lys requirement of individual, meal-fed growing pigs. In Exp. 1, we studied adaptation processes that occur during such a dose-response study in meal-fed pigs, and in Exp. 2, we studied the accuracy of this simplified technique to estimate changes in Lys requirement estimates of pigs following changes in energy intake. In Exp. 1, the effect of the Lys supply strategy on the Lys requirement was assessed in 14 barrows fed an increasing [low to high (LH)] or decreasing [high to low (HL)] total Lys supply, with total Lys levels varying from 0.36 to 1.06 g/MJ DE in 7 equidistant steps of 4 d each. Urinary urea and ammonia excretion and whole body N turnover were measured. In Exp. 2, the accuracy of the dose-response technique to determine a shift in Lys requirement was assessed in 20 barrows fed at either 2.2 [low energy (LE)] or 2.7 [high energy (HE)] times the energy requirements for maintenance, with total Lys supply decreasing from 1.10 to 0.37 g Lys/MJ DE in 9 equidistant steps of 3 d each. In Exp. 1, a lower increment in protein synthesis, breakdown, and whole body N turnover with increasing dietary Lys supply was observed in LH pigs than HL pigs (P <0.01) and the estimated Lys requirement was 0.06 g/MJ DE greater (P = 0.01) in LH pigs than HL pigs. These results indicated that pigs at a decreasing Lys supply strategy require less time for metabolic adaptation to a change in Lys supply than those at an increasing Lys supply. In Exp. 2, the estimated Lys requirement was 2.6 g/d greater (P <0.001) in HE pigs than LE pigs. The variation in AA requirement estimates between individual pigs was low (4.9% in LH pigs and 3.0% in HL pigs in Exp. 1 and 8.1% in LE pigs and 6.0% in HE pigs in Exp. 2). The present studies indicated that a dose-response technique with a decreasing Lys supply in time and a step length of 3 d with urinary N excretion as response criteria provides a simple, accurate technique to quantitatively estimate a change in AA requirements of individual meal-fed pigs.
LanguageEnglish
Pages4788-4796
JournalJournal of Animal Science
Volume91
Issue number10
DOIs
Publication statusPublished - 2013

Fingerprint

amino acid requirements
dose response
Meals
Swine
Amino Acids
swine
amino acids
methodology
energy
barrows
excretion
Energy Intake
Ammonia
energy requirements

Keywords

  • body protein-turnover
  • growing-pigs
  • oxidation technique
  • lysine requirement
  • young-pigs
  • indicator
  • nitrogen
  • variability
  • growth

Cite this

@article{537231364e56470f9cbdbf12ab8d2ae2,
title = "A simple amino acid dose-response technique to quantify amino acid requirements of individual meal-fed pigs",
abstract = "Two experiments were conducted to develop a simplified dose-response technique to estimate the Lys requirement of individual, meal-fed growing pigs. In Exp. 1, we studied adaptation processes that occur during such a dose-response study in meal-fed pigs, and in Exp. 2, we studied the accuracy of this simplified technique to estimate changes in Lys requirement estimates of pigs following changes in energy intake. In Exp. 1, the effect of the Lys supply strategy on the Lys requirement was assessed in 14 barrows fed an increasing [low to high (LH)] or decreasing [high to low (HL)] total Lys supply, with total Lys levels varying from 0.36 to 1.06 g/MJ DE in 7 equidistant steps of 4 d each. Urinary urea and ammonia excretion and whole body N turnover were measured. In Exp. 2, the accuracy of the dose-response technique to determine a shift in Lys requirement was assessed in 20 barrows fed at either 2.2 [low energy (LE)] or 2.7 [high energy (HE)] times the energy requirements for maintenance, with total Lys supply decreasing from 1.10 to 0.37 g Lys/MJ DE in 9 equidistant steps of 3 d each. In Exp. 1, a lower increment in protein synthesis, breakdown, and whole body N turnover with increasing dietary Lys supply was observed in LH pigs than HL pigs (P <0.01) and the estimated Lys requirement was 0.06 g/MJ DE greater (P = 0.01) in LH pigs than HL pigs. These results indicated that pigs at a decreasing Lys supply strategy require less time for metabolic adaptation to a change in Lys supply than those at an increasing Lys supply. In Exp. 2, the estimated Lys requirement was 2.6 g/d greater (P <0.001) in HE pigs than LE pigs. The variation in AA requirement estimates between individual pigs was low (4.9{\%} in LH pigs and 3.0{\%} in HL pigs in Exp. 1 and 8.1{\%} in LE pigs and 6.0{\%} in HE pigs in Exp. 2). The present studies indicated that a dose-response technique with a decreasing Lys supply in time and a step length of 3 d with urinary N excretion as response criteria provides a simple, accurate technique to quantitatively estimate a change in AA requirements of individual meal-fed pigs.",
keywords = "body protein-turnover, growing-pigs, oxidation technique, lysine requirement, young-pigs, indicator, nitrogen, variability, growth",
author = "{Kampman-van de Hoek}, E. and W.J.J. Gerrits and {van der Peet-Schwering}, C.M.C. and A.J.M. Jansman and {van den Borne}, J.J.G.C.",
year = "2013",
doi = "10.2527/jas.2013-6247",
language = "English",
volume = "91",
pages = "4788--4796",
journal = "Journal of Animal Science",
issn = "0021-8812",
publisher = "American Society of Animal Science",
number = "10",

}

A simple amino acid dose-response technique to quantify amino acid requirements of individual meal-fed pigs. / Kampman-van de Hoek, E.; Gerrits, W.J.J.; van der Peet-Schwering, C.M.C.; Jansman, A.J.M.; van den Borne, J.J.G.C.

In: Journal of Animal Science, Vol. 91, No. 10, 2013, p. 4788-4796.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - A simple amino acid dose-response technique to quantify amino acid requirements of individual meal-fed pigs

AU - Kampman-van de Hoek, E.

AU - Gerrits, W.J.J.

AU - van der Peet-Schwering, C.M.C.

AU - Jansman, A.J.M.

AU - van den Borne, J.J.G.C.

PY - 2013

Y1 - 2013

N2 - Two experiments were conducted to develop a simplified dose-response technique to estimate the Lys requirement of individual, meal-fed growing pigs. In Exp. 1, we studied adaptation processes that occur during such a dose-response study in meal-fed pigs, and in Exp. 2, we studied the accuracy of this simplified technique to estimate changes in Lys requirement estimates of pigs following changes in energy intake. In Exp. 1, the effect of the Lys supply strategy on the Lys requirement was assessed in 14 barrows fed an increasing [low to high (LH)] or decreasing [high to low (HL)] total Lys supply, with total Lys levels varying from 0.36 to 1.06 g/MJ DE in 7 equidistant steps of 4 d each. Urinary urea and ammonia excretion and whole body N turnover were measured. In Exp. 2, the accuracy of the dose-response technique to determine a shift in Lys requirement was assessed in 20 barrows fed at either 2.2 [low energy (LE)] or 2.7 [high energy (HE)] times the energy requirements for maintenance, with total Lys supply decreasing from 1.10 to 0.37 g Lys/MJ DE in 9 equidistant steps of 3 d each. In Exp. 1, a lower increment in protein synthesis, breakdown, and whole body N turnover with increasing dietary Lys supply was observed in LH pigs than HL pigs (P <0.01) and the estimated Lys requirement was 0.06 g/MJ DE greater (P = 0.01) in LH pigs than HL pigs. These results indicated that pigs at a decreasing Lys supply strategy require less time for metabolic adaptation to a change in Lys supply than those at an increasing Lys supply. In Exp. 2, the estimated Lys requirement was 2.6 g/d greater (P <0.001) in HE pigs than LE pigs. The variation in AA requirement estimates between individual pigs was low (4.9% in LH pigs and 3.0% in HL pigs in Exp. 1 and 8.1% in LE pigs and 6.0% in HE pigs in Exp. 2). The present studies indicated that a dose-response technique with a decreasing Lys supply in time and a step length of 3 d with urinary N excretion as response criteria provides a simple, accurate technique to quantitatively estimate a change in AA requirements of individual meal-fed pigs.

AB - Two experiments were conducted to develop a simplified dose-response technique to estimate the Lys requirement of individual, meal-fed growing pigs. In Exp. 1, we studied adaptation processes that occur during such a dose-response study in meal-fed pigs, and in Exp. 2, we studied the accuracy of this simplified technique to estimate changes in Lys requirement estimates of pigs following changes in energy intake. In Exp. 1, the effect of the Lys supply strategy on the Lys requirement was assessed in 14 barrows fed an increasing [low to high (LH)] or decreasing [high to low (HL)] total Lys supply, with total Lys levels varying from 0.36 to 1.06 g/MJ DE in 7 equidistant steps of 4 d each. Urinary urea and ammonia excretion and whole body N turnover were measured. In Exp. 2, the accuracy of the dose-response technique to determine a shift in Lys requirement was assessed in 20 barrows fed at either 2.2 [low energy (LE)] or 2.7 [high energy (HE)] times the energy requirements for maintenance, with total Lys supply decreasing from 1.10 to 0.37 g Lys/MJ DE in 9 equidistant steps of 3 d each. In Exp. 1, a lower increment in protein synthesis, breakdown, and whole body N turnover with increasing dietary Lys supply was observed in LH pigs than HL pigs (P <0.01) and the estimated Lys requirement was 0.06 g/MJ DE greater (P = 0.01) in LH pigs than HL pigs. These results indicated that pigs at a decreasing Lys supply strategy require less time for metabolic adaptation to a change in Lys supply than those at an increasing Lys supply. In Exp. 2, the estimated Lys requirement was 2.6 g/d greater (P <0.001) in HE pigs than LE pigs. The variation in AA requirement estimates between individual pigs was low (4.9% in LH pigs and 3.0% in HL pigs in Exp. 1 and 8.1% in LE pigs and 6.0% in HE pigs in Exp. 2). The present studies indicated that a dose-response technique with a decreasing Lys supply in time and a step length of 3 d with urinary N excretion as response criteria provides a simple, accurate technique to quantitatively estimate a change in AA requirements of individual meal-fed pigs.

KW - body protein-turnover

KW - growing-pigs

KW - oxidation technique

KW - lysine requirement

KW - young-pigs

KW - indicator

KW - nitrogen

KW - variability

KW - growth

U2 - 10.2527/jas.2013-6247

DO - 10.2527/jas.2013-6247

M3 - Article

VL - 91

SP - 4788

EP - 4796

JO - Journal of Animal Science

T2 - Journal of Animal Science

JF - Journal of Animal Science

SN - 0021-8812

IS - 10

ER -