A short-term intervention with selenium affects expression of genes implicated in the epithelial-to-mesenchymal transition in the prostate

Dieuwertje van Gils-Kok, Lambertus A.L.M. Kiemeney, Gerald W. Verhaegh, Jack A. Schalken, Emile N.J.T. van Lin, J.P.M. Sedelaar, J.A. Witjes, Christina A. Hulsbergen-van de Kaa, Pieter van 't Veer, Ellen Kampman, Lydia A. Afman*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

In parallel with the inconsistency in observational studies and chemoprevention trials, the mechanisms by which selenium affects prostate cancer risk have not been elucidated. We conducted a randomized, placebo-controlled trial to examine the effects of a short-term intervention with selenium on gene expression in non-malignant prostate tissue. Twenty-three men received 300 μg selenium per day in the form of selenized yeast (n=12) or a placebo (n=11) during 5 weeks. Prostate biopsies collected from the transition zone before and after intervention were analysed for 15 participants (n=8 selenium, n=7 placebo). Pathway analyses revealed that the intervention with selenium was associated with down-regulated expression of genes involved in cellular migration, invasion, remodeling and immune responses. Specifically, expression of well-established epithelial markers, such as E-cadherin and epithelial cell adhesion molecule EPCAM, was up-regulated, while the mesenchymal markers vimentin and fibronectin were down-regulated after intervention with selenium. This implies an inhibitory effect of selenium on the epithelial-to-mesenchymal transition (EMT). Moreover, selenium was associated with down-regulated expression of genes involved in wound healing and inflammation; processes which are both related to EMT. In conclusion, our explorative data showed that selenium affected expression of genes implicated in EMT in the transition zone of the prostate.

Original languageEnglish
Pages (from-to)10565-10579
JournalOncotarget
Volume8
Issue number6
DOIs
Publication statusPublished - 2017

Keywords

  • EMT
  • Gene expression
  • Microarray
  • Prostatic neoplasms
  • Selenium

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    A short-term intervention with selenium affects expression of genes implicated in the epithelial-to-mesenchymal transition in the prostate

    Kok, D. (Creator), Kiemeney, L. A. L. M. (Creator), Verhaegh, G. W. (Creator), Schalken, J. A. (Creator), van Lin, E. N. J. T. (Creator), Sedelaar, J. P. M. (Creator), Witjes, J. A. (Creator), Hulsbergen-van de Kaa, C. A. (Creator), van 't Veer, P. (Creator), Kampman, E. (Creator) & Afman, L. (Creator), Wageningen University, 19 Jan 2017

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    Cite this

    van Gils-Kok, D., Kiemeney, L. A. L. M., Verhaegh, G. W., Schalken, J. A., van Lin, E. N. J. T., Sedelaar, J. P. M., Witjes, J. A., Hulsbergen-van de Kaa, C. A., van 't Veer, P., Kampman, E., & Afman, L. A. (2017). A short-term intervention with selenium affects expression of genes implicated in the epithelial-to-mesenchymal transition in the prostate. Oncotarget, 8(6), 10565-10579. https://doi.org/10.18632/oncotarget.14551