A sensitive epitope-blocking ELISA for the detection of Chikungunya virus-specific antibodies in patients

L.Y.H. Goh; Y.W. Kam; S.W.H. Metz; J. Hobson-Peters; N.A. Prow; S. McCarthy; D.W. Smith; G.P. Pijlman; L.F.P. Ng; R.A. Hall

doi:10.1016/j.jviromet.2015.05.011

A sensitive epitope-blocking ELISA for the detection of Chikungunya virus-specific antibodies in patients

L.Y.H. Goh, Y.W. Kam, S.W.H. Metz, J. Hobson-Peters, N.A. Prow, S. McCarthy, D.W. Smith, G.P. Pijlman, L.F.P. Ng, R.A. Hall

Research output: Contribution to journal › Article › Academic › peer-review

6 Citations (Scopus)

Abstract

Chikungunya fever (CHIKF) has re-emerged as an arboviral disease that mimics clinical symptoms of other diseases such as dengue, malaria, as well as other alphavirus-related illnesses leading to problems with definitive diagnosis of the infection. Herein we describe the development and evaluation of a sensitive epitope-blocking ELISA (EB-ELISA) capable of specifically detecting anti-chikungunya virus (CHIKV) antibodies in clinical samples. The assay uses a monoclonal antibody (mAb) that binds an epitope on the E2 protein of CHIKV and does not exhibit cross-reactivity to other related alphaviruses. We also demonstrated the use of recombinant CHIK virus-like particles (VLPs) as a safe alternative antigen to infectious virions in the assay. Based on testing of 60 serum samples from patients in the acute or convalescent phase of CHIKV infection, the EB-ELISA provided us with 100% sensitivity, and exhibited 98.5% specificity when Ross River virus (RRV)- or Barmah Forest virus (BFV)-immune serum samples were included. This assay meets the public health demands of a rapid, robust, sensitive and specific, yet simple assay for specifically diagnosing CHIK-infections in humans.

Original language	English
Pages (from-to)	55-61
Journal	Journal of Virological Methods
Volume	222
DOIs	https://doi.org/10.1016/j.jviromet.2015.05.011
Publication status	Published - 2015

Keywords

west-nile-virus
linked-immunosorbent-assay
valley encephalitis-virus
monoclonal-antibodies
diagnostic-accuracy
universal detection
reunion island
insect cells
ns1 protein
pcr assay

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@article{f91e09b2ac0e48f99754892febbf1e3a,

title = "A sensitive epitope-blocking ELISA for the detection of Chikungunya virus-specific antibodies in patients",

abstract = "Chikungunya fever (CHIKF) has re-emerged as an arboviral disease that mimics clinical symptoms of other diseases such as dengue, malaria, as well as other alphavirus-related illnesses leading to problems with definitive diagnosis of the infection. Herein we describe the development and evaluation of a sensitive epitope-blocking ELISA (EB-ELISA) capable of specifically detecting anti-chikungunya virus (CHIKV) antibodies in clinical samples. The assay uses a monoclonal antibody (mAb) that binds an epitope on the E2 protein of CHIKV and does not exhibit cross-reactivity to other related alphaviruses. We also demonstrated the use of recombinant CHIK virus-like particles (VLPs) as a safe alternative antigen to infectious virions in the assay. Based on testing of 60 serum samples from patients in the acute or convalescent phase of CHIKV infection, the EB-ELISA provided us with 100% sensitivity, and exhibited 98.5% specificity when Ross River virus (RRV)- or Barmah Forest virus (BFV)-immune serum samples were included. This assay meets the public health demands of a rapid, robust, sensitive and specific, yet simple assay for specifically diagnosing CHIK-infections in humans.",

keywords = "west-nile-virus, linked-immunosorbent-assay, valley encephalitis-virus, monoclonal-antibodies, diagnostic-accuracy, universal detection, reunion island, insect cells, ns1 protein, pcr assay",

author = "L.Y.H. Goh and Y.W. Kam and S.W.H. Metz and J. Hobson-Peters and N.A. Prow and S. McCarthy and D.W. Smith and G.P. Pijlman and L.F.P. Ng and R.A. Hall",

year = "2015",

doi = "10.1016/j.jviromet.2015.05.011",

language = "English",

volume = "222",

pages = "55--61",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier",

}

A sensitive epitope-blocking ELISA for the detection of Chikungunya virus-specific antibodies in patients. / Goh, L.Y.H.; Kam, Y.W.; Metz, S.W.H.; Hobson-Peters, J.; Prow, N.A.; McCarthy, S.; Smith, D.W.; Pijlman, G.P.; Ng, L.F.P.; Hall, R.A.

In: Journal of Virological Methods, Vol. 222, 2015, p. 55-61.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - A sensitive epitope-blocking ELISA for the detection of Chikungunya virus-specific antibodies in patients

AU - Goh, L.Y.H.

AU - Kam, Y.W.

AU - Metz, S.W.H.

AU - Hobson-Peters, J.

AU - Prow, N.A.

AU - McCarthy, S.

AU - Smith, D.W.

AU - Pijlman, G.P.

AU - Ng, L.F.P.

AU - Hall, R.A.

PY - 2015

Y1 - 2015

N2 - Chikungunya fever (CHIKF) has re-emerged as an arboviral disease that mimics clinical symptoms of other diseases such as dengue, malaria, as well as other alphavirus-related illnesses leading to problems with definitive diagnosis of the infection. Herein we describe the development and evaluation of a sensitive epitope-blocking ELISA (EB-ELISA) capable of specifically detecting anti-chikungunya virus (CHIKV) antibodies in clinical samples. The assay uses a monoclonal antibody (mAb) that binds an epitope on the E2 protein of CHIKV and does not exhibit cross-reactivity to other related alphaviruses. We also demonstrated the use of recombinant CHIK virus-like particles (VLPs) as a safe alternative antigen to infectious virions in the assay. Based on testing of 60 serum samples from patients in the acute or convalescent phase of CHIKV infection, the EB-ELISA provided us with 100% sensitivity, and exhibited 98.5% specificity when Ross River virus (RRV)- or Barmah Forest virus (BFV)-immune serum samples were included. This assay meets the public health demands of a rapid, robust, sensitive and specific, yet simple assay for specifically diagnosing CHIK-infections in humans.

AB - Chikungunya fever (CHIKF) has re-emerged as an arboviral disease that mimics clinical symptoms of other diseases such as dengue, malaria, as well as other alphavirus-related illnesses leading to problems with definitive diagnosis of the infection. Herein we describe the development and evaluation of a sensitive epitope-blocking ELISA (EB-ELISA) capable of specifically detecting anti-chikungunya virus (CHIKV) antibodies in clinical samples. The assay uses a monoclonal antibody (mAb) that binds an epitope on the E2 protein of CHIKV and does not exhibit cross-reactivity to other related alphaviruses. We also demonstrated the use of recombinant CHIK virus-like particles (VLPs) as a safe alternative antigen to infectious virions in the assay. Based on testing of 60 serum samples from patients in the acute or convalescent phase of CHIKV infection, the EB-ELISA provided us with 100% sensitivity, and exhibited 98.5% specificity when Ross River virus (RRV)- or Barmah Forest virus (BFV)-immune serum samples were included. This assay meets the public health demands of a rapid, robust, sensitive and specific, yet simple assay for specifically diagnosing CHIK-infections in humans.

KW - west-nile-virus

KW - linked-immunosorbent-assay

KW - valley encephalitis-virus

KW - monoclonal-antibodies

KW - diagnostic-accuracy

KW - universal detection

KW - reunion island

KW - insect cells

KW - ns1 protein

KW - pcr assay

U2 - 10.1016/j.jviromet.2015.05.011

DO - 10.1016/j.jviromet.2015.05.011

M3 - Article

VL - 222

SP - 55

EP - 61

JO - Journal of Virological Methods

JF - Journal of Virological Methods

SN - 0166-0934

ER -