A self-consistent field modelling of the neurofilament network

We have investigated, on a self-consistent field level, the equilibrium structure of the neurofilament network formed by the NF-H, NF-M and NF-L proteins, using the one-gradient version of the numerical model of Scheutjens and Fleer. We demonstrate a reticulation of NFs in parallel bundles that occurs due to hydrophobic attractions between apolar aminoacid residues in the terminal parts of the M- and H-tails. We elaborate on the feasibility that the stability of the NF network can be enhanced by specific interactions between the projection domains, possibly induced by accessary proteins. We demonstrate that the phosphorylation of KSP repeats in the M- and H-tails promotes the cross-bridging between the NFs and therefore helps form the NF network