A second HD mating type sublocus of Flammulina velutipes is at least di-allelic and active: New primers for identification of HD - A and HD-b subloci

Wei Wang, Irum Mukhtar, Tiansheng Chou, Siyuan Jiang, Xinrui Liu, Arend F. Van Peer, Baogui Xie

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Background: Sexual development in Flammulina velutipes is controlled by two different mating type loci (HD and PR). The HD locus contains homeodomain (Hd) genes on two separate HD subloci: HD-a and HD-b. While the functionality of the HD-b sublocus has been largely confirmed, the status and content of the HD-a sublocus has remained unclear. Methods: To examine the function of the HD-a sublocus, genome sequences of a series of F. velutipes strains were analyzed and tested through series of amplification by specific primer sets. Furthermore, activity of di-allelic HD-a locus was confirmed by crossing strains with different combinations of HD-a and HD-b subloci. Results: Sublocus HD-b contained a large variety of fixed Hd1/Hd2 gene pairs, while the HD-a sublocus either contained a conserved Hd2 gene or, a newly discovered Hd1 gene that was also conserved. Identification of whole HD loci, that is, the contents of HD-a and HD-b subloci in a strain, revealed that strains with similar HD-b subloci could still form normal dikaryons if the two genes at the HD-a sublocus differed. At least di-allelic HD-a sublocus, is thus indicated to be actively involved in mating type compatibility. Conclusions: HD-a sublocus is active and di-allelic. Using the new information on the HD subloci, primers sets were developed that specifically amplify HD-a or HD-b subloci in the majority of F. velutipes strains. In this way, unknown HD mating types of F. velutipes can now be quickly identified, and HD mating type compatibility conferred by HD-a or HD-b can be confirmed by PCR.

LanguageEnglish
Article numbere6292
JournalPeerJ
Volume2019
Issue number2
DOIs
Publication statusPublished - 22 Feb 2019

Fingerprint

Flammulina
Flammulina velutipes
Genes
loci
genes
Sexual Development
sexual development
Amplification
mating types
Genome
Polymerase Chain Reaction
genome

Keywords

  • Di-allelic
  • Flammulina velutipes
  • HD subloci
  • Homeodomain
  • Mating pathway
  • Primers

Cite this

Wang, Wei ; Mukhtar, Irum ; Chou, Tiansheng ; Jiang, Siyuan ; Liu, Xinrui ; Van Peer, Arend F. ; Xie, Baogui. / A second HD mating type sublocus of Flammulina velutipes is at least di-allelic and active : New primers for identification of HD - A and HD-b subloci. In: PeerJ. 2019 ; Vol. 2019, No. 2.
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title = "A second HD mating type sublocus of Flammulina velutipes is at least di-allelic and active: New primers for identification of HD - A and HD-b subloci",
abstract = "Background: Sexual development in Flammulina velutipes is controlled by two different mating type loci (HD and PR). The HD locus contains homeodomain (Hd) genes on two separate HD subloci: HD-a and HD-b. While the functionality of the HD-b sublocus has been largely confirmed, the status and content of the HD-a sublocus has remained unclear. Methods: To examine the function of the HD-a sublocus, genome sequences of a series of F. velutipes strains were analyzed and tested through series of amplification by specific primer sets. Furthermore, activity of di-allelic HD-a locus was confirmed by crossing strains with different combinations of HD-a and HD-b subloci. Results: Sublocus HD-b contained a large variety of fixed Hd1/Hd2 gene pairs, while the HD-a sublocus either contained a conserved Hd2 gene or, a newly discovered Hd1 gene that was also conserved. Identification of whole HD loci, that is, the contents of HD-a and HD-b subloci in a strain, revealed that strains with similar HD-b subloci could still form normal dikaryons if the two genes at the HD-a sublocus differed. At least di-allelic HD-a sublocus, is thus indicated to be actively involved in mating type compatibility. Conclusions: HD-a sublocus is active and di-allelic. Using the new information on the HD subloci, primers sets were developed that specifically amplify HD-a or HD-b subloci in the majority of F. velutipes strains. In this way, unknown HD mating types of F. velutipes can now be quickly identified, and HD mating type compatibility conferred by HD-a or HD-b can be confirmed by PCR.",
keywords = "Di-allelic, Flammulina velutipes, HD subloci, Homeodomain, Mating pathway, Primers",
author = "Wei Wang and Irum Mukhtar and Tiansheng Chou and Siyuan Jiang and Xinrui Liu and {Van Peer}, {Arend F.} and Baogui Xie",
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A second HD mating type sublocus of Flammulina velutipes is at least di-allelic and active : New primers for identification of HD - A and HD-b subloci. / Wang, Wei; Mukhtar, Irum; Chou, Tiansheng; Jiang, Siyuan; Liu, Xinrui; Van Peer, Arend F.; Xie, Baogui.

In: PeerJ, Vol. 2019, No. 2, e6292, 22.02.2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - A second HD mating type sublocus of Flammulina velutipes is at least di-allelic and active

T2 - PeerJ

AU - Wang, Wei

AU - Mukhtar, Irum

AU - Chou, Tiansheng

AU - Jiang, Siyuan

AU - Liu, Xinrui

AU - Van Peer, Arend F.

AU - Xie, Baogui

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N2 - Background: Sexual development in Flammulina velutipes is controlled by two different mating type loci (HD and PR). The HD locus contains homeodomain (Hd) genes on two separate HD subloci: HD-a and HD-b. While the functionality of the HD-b sublocus has been largely confirmed, the status and content of the HD-a sublocus has remained unclear. Methods: To examine the function of the HD-a sublocus, genome sequences of a series of F. velutipes strains were analyzed and tested through series of amplification by specific primer sets. Furthermore, activity of di-allelic HD-a locus was confirmed by crossing strains with different combinations of HD-a and HD-b subloci. Results: Sublocus HD-b contained a large variety of fixed Hd1/Hd2 gene pairs, while the HD-a sublocus either contained a conserved Hd2 gene or, a newly discovered Hd1 gene that was also conserved. Identification of whole HD loci, that is, the contents of HD-a and HD-b subloci in a strain, revealed that strains with similar HD-b subloci could still form normal dikaryons if the two genes at the HD-a sublocus differed. At least di-allelic HD-a sublocus, is thus indicated to be actively involved in mating type compatibility. Conclusions: HD-a sublocus is active and di-allelic. Using the new information on the HD subloci, primers sets were developed that specifically amplify HD-a or HD-b subloci in the majority of F. velutipes strains. In this way, unknown HD mating types of F. velutipes can now be quickly identified, and HD mating type compatibility conferred by HD-a or HD-b can be confirmed by PCR.

AB - Background: Sexual development in Flammulina velutipes is controlled by two different mating type loci (HD and PR). The HD locus contains homeodomain (Hd) genes on two separate HD subloci: HD-a and HD-b. While the functionality of the HD-b sublocus has been largely confirmed, the status and content of the HD-a sublocus has remained unclear. Methods: To examine the function of the HD-a sublocus, genome sequences of a series of F. velutipes strains were analyzed and tested through series of amplification by specific primer sets. Furthermore, activity of di-allelic HD-a locus was confirmed by crossing strains with different combinations of HD-a and HD-b subloci. Results: Sublocus HD-b contained a large variety of fixed Hd1/Hd2 gene pairs, while the HD-a sublocus either contained a conserved Hd2 gene or, a newly discovered Hd1 gene that was also conserved. Identification of whole HD loci, that is, the contents of HD-a and HD-b subloci in a strain, revealed that strains with similar HD-b subloci could still form normal dikaryons if the two genes at the HD-a sublocus differed. At least di-allelic HD-a sublocus, is thus indicated to be actively involved in mating type compatibility. Conclusions: HD-a sublocus is active and di-allelic. Using the new information on the HD subloci, primers sets were developed that specifically amplify HD-a or HD-b subloci in the majority of F. velutipes strains. In this way, unknown HD mating types of F. velutipes can now be quickly identified, and HD mating type compatibility conferred by HD-a or HD-b can be confirmed by PCR.

KW - Di-allelic

KW - Flammulina velutipes

KW - HD subloci

KW - Homeodomain

KW - Mating pathway

KW - Primers

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