A RP-HPLC method for the determination of tea catechins

S. Khokhar, D. Venema, P.C.H. Hollman, M. Dekker, W.M.F. Jongen

Research output: Contribution to journalArticleAcademicpeer-review

89 Citations (Scopus)

Abstract

An HPLC method with gradient elution for the quantification of catechins ((−)-epigallocatechin (EGC), ( )-catechin (C), (−)-epicatechin (EC), (−)-epigallocatechingallate (EGCg) and (−)-epicatechingallate (ECg)) in tea was developed. The method was used to determine catechins in black tea, green tea and oolong tea. Sample preparation was simple because only filtration and adjustment of pH was required. The levels of catechins in different teas varied between <5 and 287 (EGC), 17 and 94 (EC), 18 and 408 (EGCg), and 8 and 85 mg/l (ECg). ( )-Catechin was not present in any of these teas.
Original languageEnglish
Pages (from-to)171-172
JournalCancer Letters
Volume114
Issue number1-2
DOIs
Publication statusPublished - 1997

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