A RP-HPLC method for the determination of tea catechins

S. Khokhar; D. Venema; P.C.H. Hollman; M. Dekker; W.M.F. Jongen

doi:10.1016/S0304-3835(97)04653-3

A RP-HPLC method for the determination of tea catechins

S. Khokhar, D. Venema, P.C.H. Hollman, M. Dekker, W.M.F. Jongen

Wageningen Food Safety Research

Research output: Contribution to journal › Article › Academic › peer-review

94 Citations (Scopus)

Abstract

An HPLC method with gradient elution for the quantification of catechins ((−)-epigallocatechin (EGC), ( )-catechin (C), (−)-epicatechin (EC), (−)-epigallocatechingallate (EGCg) and (−)-epicatechingallate (ECg)) in tea was developed. The method was used to determine catechins in black tea, green tea and oolong tea. Sample preparation was simple because only filtration and adjustment of pH was required. The levels of catechins in different teas varied between <5 and 287 (EGC), 17 and 94 (EC), 18 and 408 (EGCg), and 8 and 85 mg/l (ECg). ( )-Catechin was not present in any of these teas.

Original language	English
Pages (from-to)	171-172
Journal	Cancer Letters
Volume	114
Issue number	1-2
DOIs	https://doi.org/10.1016/S0304-3835(97)04653-3
Publication status	Published - 1997

Keywords

Analysis
Antioxidant
Catechins
HPLC
Tea

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0304-3835(97)04653-3

Cite this

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title = "A RP-HPLC method for the determination of tea catechins",

abstract = "An HPLC method with gradient elution for the quantification of catechins ((−)-epigallocatechin (EGC), ( )-catechin (C), (−)-epicatechin (EC), (−)-epigallocatechingallate (EGCg) and (−)-epicatechingallate (ECg)) in tea was developed. The method was used to determine catechins in black tea, green tea and oolong tea. Sample preparation was simple because only filtration and adjustment of pH was required. The levels of catechins in different teas varied between <5 and 287 (EGC), 17 and 94 (EC), 18 and 408 (EGCg), and 8 and 85 mg/l (ECg). ( )-Catechin was not present in any of these teas.",

keywords = "Analysis, Antioxidant, Catechins, HPLC, Tea",

author = "S. Khokhar and D. Venema and P.C.H. Hollman and M. Dekker and W.M.F. Jongen",

year = "1997",

doi = "10.1016/S0304-3835(97)04653-3",

language = "English",

volume = "114",

pages = "171--172",

journal = "Cancer Letters",

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TY - JOUR

T1 - A RP-HPLC method for the determination of tea catechins

AU - Khokhar, S.

AU - Venema, D.

AU - Hollman, P.C.H.

AU - Dekker, M.

AU - Jongen, W.M.F.

PY - 1997

Y1 - 1997

N2 - An HPLC method with gradient elution for the quantification of catechins ((−)-epigallocatechin (EGC), ( )-catechin (C), (−)-epicatechin (EC), (−)-epigallocatechingallate (EGCg) and (−)-epicatechingallate (ECg)) in tea was developed. The method was used to determine catechins in black tea, green tea and oolong tea. Sample preparation was simple because only filtration and adjustment of pH was required. The levels of catechins in different teas varied between <5 and 287 (EGC), 17 and 94 (EC), 18 and 408 (EGCg), and 8 and 85 mg/l (ECg). ( )-Catechin was not present in any of these teas.

AB - An HPLC method with gradient elution for the quantification of catechins ((−)-epigallocatechin (EGC), ( )-catechin (C), (−)-epicatechin (EC), (−)-epigallocatechingallate (EGCg) and (−)-epicatechingallate (ECg)) in tea was developed. The method was used to determine catechins in black tea, green tea and oolong tea. Sample preparation was simple because only filtration and adjustment of pH was required. The levels of catechins in different teas varied between <5 and 287 (EGC), 17 and 94 (EC), 18 and 408 (EGCg), and 8 and 85 mg/l (ECg). ( )-Catechin was not present in any of these teas.

KW - Analysis

KW - Antioxidant

KW - Catechins

KW - HPLC

KW - Tea

U2 - 10.1016/S0304-3835(97)04653-3

DO - 10.1016/S0304-3835(97)04653-3

M3 - Article

SN - 0304-3835

VL - 114

SP - 171

EP - 172

JO - Cancer Letters

JF - Cancer Letters

IS - 1-2

ER -

A RP-HPLC method for the determination of tea catechins

Abstract

Keywords

UN SDGs

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