A role for ColV plasmids in the evolution of pathogenic Escherichia coli ST58

Cameron J. Reid; Max L. Cummins; Stefan Börjesson; Michael S.M. Brouwer; Henrik Hasman; Anette M. Hammerum; Louise Roer; Stefanie Hess; Thomas Berendonk; Kristina Nešporová; Marisa Haenni; Jean Yves Madec; Astrid Bethe; Geovana B. Michael; Anne Kathrin Schink; Stefan Schwarz; Monika Dolejska; Steven P. Djordjevic

doi:10.1038/s41467-022-28342-4

A role for ColV plasmids in the evolution of pathogenic Escherichia coli ST58

Cameron J. Reid^*, Max L. Cummins, Stefan Börjesson, Michael S.M. Brouwer, Henrik Hasman, Anette M. Hammerum, Louise Roer, Stefanie Hess, Thomas Berendonk, Kristina Nešporová, Marisa Haenni, Jean Yves Madec, Astrid Bethe, Geovana B. Michael, Anne Kathrin Schink, Stefan Schwarz, Monika Dolejska, Steven P. Djordjevic

^*Corresponding author for this work

Bacteriology, Host Pathogen Interaction & Diagnostics

Research output: Contribution to journal › Article › Academic › peer-review

49 Citations (Scopus)

Abstract

Escherichia coli ST58 has recently emerged as a globally disseminated uropathogen that often progresses to sepsis. Unlike most pandemic extra-intestinal pathogenic E. coli (ExPEC), which belong to pathogenic phylogroup B2, ST58 belongs to the environmental/commensal phylogroup B1. Here, we present a pan-genomic analysis of a global collection of 752 ST58 isolates from diverse sources. We identify a large ST58 sub-lineage characterized by near ubiquitous carriage of ColV plasmids, which carry genes encoding virulence factors, and by a distinct accessory genome including genes typical of the Yersiniabactin High Pathogenicity Island. This sub-lineage includes three-quarters of all ExPEC sequences in our study and has a broad host range, although poultry and porcine sources predominate. By contrast, strains isolated from cattle often lack ColV plasmids. Our data indicate that ColV plasmid acquisition contributed to the divergence of the major ST58 sub-lineage, and different sub-lineages inhabit poultry, swine and cattle.

Original language	English
Article number	683
Journal	Nature Communications
Volume	13
Issue number	1
DOIs	https://doi.org/10.1038/s41467-022-28342-4
Publication status	Published - Dec 2022

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https://edepot.wur.nl/565285Licence: CC BY

Escherichia coli ST58
Reid, C. J. (Creator), Cummins, M. L. (Creator), Börjesson, S. (Creator), Brouwer, M. S. M. (Creator), Hasman, H. (Creator), Hammerum, A. M. (Creator), Roer, L. (Creator), Hess, S. (Creator), Berendonk, T. (Creator), Nešporová, K. (Creator), Haenni, M. (Creator), Madec, J. Y. (Creator), Bethe, A. (Creator), Michael, G. B. (Creator), Schink, A. K. (Creator), Schwarz, S. (Creator), Dolejska, M. (Creator) & Djordjevic, S. P. (Creator), University of Technology Sydney, 4 May 2021
https://www.ncbi.nlm.nih.gov/bioproject/727368
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Reid, C. J., Cummins, M. L., Börjesson, S., Brouwer, M. S. M., Hasman, H., Hammerum, A. M., Roer, L., Hess, S., Berendonk, T., Nešporová, K., Haenni, M., Madec, J. Y., Bethe, A., Michael, G. B., Schink, A. K., Schwarz, S., Dolejska, M., & Djordjevic, S. P. (2022). A role for ColV plasmids in the evolution of pathogenic Escherichia coli ST58. Nature Communications, 13(1), Article 683. https://doi.org/10.1038/s41467-022-28342-4

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abstract = "Escherichia coli ST58 has recently emerged as a globally disseminated uropathogen that often progresses to sepsis. Unlike most pandemic extra-intestinal pathogenic E. coli (ExPEC), which belong to pathogenic phylogroup B2, ST58 belongs to the environmental/commensal phylogroup B1. Here, we present a pan-genomic analysis of a global collection of 752 ST58 isolates from diverse sources. We identify a large ST58 sub-lineage characterized by near ubiquitous carriage of ColV plasmids, which carry genes encoding virulence factors, and by a distinct accessory genome including genes typical of the Yersiniabactin High Pathogenicity Island. This sub-lineage includes three-quarters of all ExPEC sequences in our study and has a broad host range, although poultry and porcine sources predominate. By contrast, strains isolated from cattle often lack ColV plasmids. Our data indicate that ColV plasmid acquisition contributed to the divergence of the major ST58 sub-lineage, and different sub-lineages inhabit poultry, swine and cattle.",

author = "Reid, {Cameron J.} and Cummins, {Max L.} and Stefan B{\"o}rjesson and Brouwer, {Michael S.M.} and Henrik Hasman and Hammerum, {Anette M.} and Louise Roer and Stefanie Hess and Thomas Berendonk and Kristina Ne{\v s}porov{\'a} and Marisa Haenni and Madec, {Jean Yves} and Astrid Bethe and Michael, {Geovana B.} and Schink, {Anne Kathrin} and Stefan Schwarz and Monika Dolejska and Djordjevic, {Steven P.}",

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Reid, CJ, Cummins, ML, Börjesson, S, Brouwer, MSM, Hasman, H, Hammerum, AM, Roer, L, Hess, S, Berendonk, T, Nešporová, K, Haenni, M, Madec, JY, Bethe, A, Michael, GB, Schink, AK, Schwarz, S, Dolejska, M & Djordjevic, SP 2022, 'A role for ColV plasmids in the evolution of pathogenic Escherichia coli ST58', Nature Communications, vol. 13, no. 1, 683. https://doi.org/10.1038/s41467-022-28342-4

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AU - Reid, Cameron J.

AU - Cummins, Max L.

AU - Börjesson, Stefan

AU - Brouwer, Michael S.M.

AU - Hasman, Henrik

AU - Hammerum, Anette M.

AU - Roer, Louise

AU - Hess, Stefanie

AU - Berendonk, Thomas

AU - Nešporová, Kristina

AU - Haenni, Marisa

AU - Madec, Jean Yves

AU - Bethe, Astrid

AU - Michael, Geovana B.

AU - Schink, Anne Kathrin

AU - Schwarz, Stefan

AU - Dolejska, Monika

AU - Djordjevic, Steven P.

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AB - Escherichia coli ST58 has recently emerged as a globally disseminated uropathogen that often progresses to sepsis. Unlike most pandemic extra-intestinal pathogenic E. coli (ExPEC), which belong to pathogenic phylogroup B2, ST58 belongs to the environmental/commensal phylogroup B1. Here, we present a pan-genomic analysis of a global collection of 752 ST58 isolates from diverse sources. We identify a large ST58 sub-lineage characterized by near ubiquitous carriage of ColV plasmids, which carry genes encoding virulence factors, and by a distinct accessory genome including genes typical of the Yersiniabactin High Pathogenicity Island. This sub-lineage includes three-quarters of all ExPEC sequences in our study and has a broad host range, although poultry and porcine sources predominate. By contrast, strains isolated from cattle often lack ColV plasmids. Our data indicate that ColV plasmid acquisition contributed to the divergence of the major ST58 sub-lineage, and different sub-lineages inhabit poultry, swine and cattle.

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A role for ColV plasmids in the evolution of pathogenic Escherichia coli ST58

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Escherichia coli ST58

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