Projects per year
Abstract
Land plants can reproduce sexually by developing an embryo from a fertilized egg cell. However, embryos can also be formed from other cell types in many plant species. A key question is thus how embryo identity in plants is controlled, and how this process is modified during non-zygotic embryogenesis. The Arabidopsis zygote divides to produce an embryonic lineage and an extra-embryonic suspensor. Yet, normally quiescent suspensor cells can develop a second embryo when the initial embryo is damaged, or when response to the signaling molecule auxin is locally blocked. Here we have used auxin-dependent suspensor embryogenesis as a model to determine transcriptome changes during embryonic reprogramming. We find that reprogramming is complex and accompanied by large transcriptomic changes prior to anatomic changes. This analysis revealed a strong enrichment for genes encoding components of auxin homeostasis and response among misregulated genes. Strikingly, deregulation among multiple auxin-related gene families converged upon re-establishment of cellular auxin levels or response. This suggests a remarkable degree of feedback regulation to create resilience in auxin response during embryo development. Starting from the transcriptome of auxin-deregulated embryos, we identify an auxin-dependent bHLH transcription factor network that mediates the activity of this hormone in suppressing embryo development from the suspensor.
Original language | English |
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Pages (from-to) | 52-67 |
Journal | The Plant Cell |
Volume | 31 |
Early online date | 20 Dec 2018 |
DOIs | |
Publication status | Published - Jan 2019 |
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A Robust Auxin Response Network Controls Embryo and Suspensor Development through a bHLH Transcriptional Module
Radoeva, T. M. (Creator), Boekschoten, M. (Creator), Hooiveld, G. (Creator) & Weijers, D. (Creator), Wageningen University, 3 Jan 2019
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE69700
Dataset
Projects
- 1 Finished
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SIREN: SIgnals and REgulatory Networks in early plant embryogenesis
1/11/08 → 31/10/12
Project: EU research project