Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 52-67 |
| Journal | The Plant Cell |
| Volume | 31 |
| Early online date | 20 Dec 2018 |
| DOIs | |
| Publication status | Published - Jan 2019 |
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A Robust Auxin Response Network Controls Embryo and Suspensor Development through a bHLH Transcriptional Module. / Radoeva, Tatyana; Lokerse, Annemarie S.; Llavata-Peris, Cristina I.; Wendrich, Jos; Xiang, Daoquan; Liao, Che-Yang; Vlaar, Lieke; Boekschoten, Mark; Hooiveld, Guido; Datla, Raju; Weijers, Dolf.
In: The Plant Cell, Vol. 31, 01.2019, p. 52-67.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - A Robust Auxin Response Network Controls Embryo and Suspensor Development through a bHLH Transcriptional Module
AU - Radoeva, Tatyana
AU - Lokerse, Annemarie S.
AU - Llavata-Peris, Cristina I.
AU - Wendrich, Jos
AU - Xiang, Daoquan
AU - Liao, Che-Yang
AU - Vlaar, Lieke
AU - Boekschoten, Mark
AU - Hooiveld, Guido
AU - Datla, Raju
AU - Weijers, Dolf
PY - 2019/1
Y1 - 2019/1
N2 - Land plants can reproduce sexually by developing an embryo from a fertilized egg cell. However, embryos can also be formed from other cell types in many plant species. A key question is thus how embryo identity in plants is controlled, and how this process is modified during non-zygotic embryogenesis. The Arabidopsis zygote divides to produce an embryonic lineage and an extra-embryonic suspensor. Yet, normally quiescent suspensor cells can develop a second embryo when the initial embryo is damaged, or when response to the signaling molecule auxin is locally blocked. Here we have used auxin-dependent suspensor embryogenesis as a model to determine transcriptome changes during embryonic reprogramming. We find that reprogramming is complex and accompanied by large transcriptomic changes prior to anatomic changes. This analysis revealed a strong enrichment for genes encoding components of auxin homeostasis and response among misregulated genes. Strikingly, deregulation among multiple auxin-related gene families converged upon re-establishment of cellular auxin levels or response. This suggests a remarkable degree of feedback regulation to create resilience in auxin response during embryo development. Starting from the transcriptome of auxin-deregulated embryos, we identify an auxin-dependent bHLH transcription factor network that mediates the activity of this hormone in suppressing embryo development from the suspensor.
AB - Land plants can reproduce sexually by developing an embryo from a fertilized egg cell. However, embryos can also be formed from other cell types in many plant species. A key question is thus how embryo identity in plants is controlled, and how this process is modified during non-zygotic embryogenesis. The Arabidopsis zygote divides to produce an embryonic lineage and an extra-embryonic suspensor. Yet, normally quiescent suspensor cells can develop a second embryo when the initial embryo is damaged, or when response to the signaling molecule auxin is locally blocked. Here we have used auxin-dependent suspensor embryogenesis as a model to determine transcriptome changes during embryonic reprogramming. We find that reprogramming is complex and accompanied by large transcriptomic changes prior to anatomic changes. This analysis revealed a strong enrichment for genes encoding components of auxin homeostasis and response among misregulated genes. Strikingly, deregulation among multiple auxin-related gene families converged upon re-establishment of cellular auxin levels or response. This suggests a remarkable degree of feedback regulation to create resilience in auxin response during embryo development. Starting from the transcriptome of auxin-deregulated embryos, we identify an auxin-dependent bHLH transcription factor network that mediates the activity of this hormone in suppressing embryo development from the suspensor.
U2 - 10.1105/tpc.18.00518
DO - 10.1105/tpc.18.00518
M3 - Article
VL - 31
SP - 52
EP - 67
JO - The Plant Cell
JF - The Plant Cell
SN - 1040-4651
ER -