The mapping resolution of the physical map for chicken chromosome 4 (GGA4) was improved by a combination of radiation hybrid (RH) mapping and BAC mapping. The ChickRH6 hybrid panel was used to construct an RH map of GGA4. Eleven microsatellites known to be located on GGA4 were included as anchors to the genetic linkage map for this chromosome. Based on the known conserved synteny between chicken GGA4 and human chromosomes 4 and X, sequences where identified for the orthologous chicken genes from these human chromosomes. These sequences were subsequently used for the development of STS markers to be typed on the RH panel. Using a lod threshold of 5.0 nine linkage groups could be constructed which were aligned with the genetic linkage map of this chromosome. The resulting RH map consisted of the 11 microsatellite markers and 50 genes. To further increase the number of genes on the map and to provide additional anchor points for the physical BAC map of this chromosome BAC clones were identified for 22 microsatellites and 99 genes. The combined RH and BAC mapping approach resulted in the mapping of 63 genes on chicken GGA4 increasing the resolution of the chicken human comparative map for this chromosome. This enhanced comparative mapping resolution enabled the identification of multiple rearrangements between chicken GGA4 and human chromosomes 4q and Xp.
|Title of host publication||Plant and Animal Genomes XII Conference, San Diego, CA, January 10-14, 2004|
|Publication status||Published - 2005|