A PXY-Mediated Transcriptional Network Integrates Signaling Mechanisms to Control Vascular Development in Arabidopsis

Margot Smit, Shauni Mcgregor, Heng Sun, Catherine Gough, Anne-Maarit Bågman, Cara L. Soyars, Johan T.M. Kroon, Allison Gaudinier, Clara J. Williams, Xiyan Yang, Zachary L. Nimchuk, Dolf Weijers, Simon R. Turner, Siobhan M. Brady, Peter Etchells

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Vascular meristems generate the majority of biomass in higher plants. They constitute a bifacial stem cell population from which xylem and phloem are specified on opposing sides by positional signals. The PHLOEM INTERCALATED WITH XYLEM (PXY) receptor kinase promotes vascular cell division and organisation. However, how these functions are specified and integrated is unknown. Here, a putative PXY-mediated transcriptional regulatory network comprised of 690 transcription factor-promoter interactions was mapped. Among these interactions was a feed-forward loop containing transcription factors WUSCHEL HOMEOBOX RELATED 14 (WOX14) and TARGET OF MONOPTEROS 6 (TMO6), which each regulate the expression of a third transcription factor, LATERAL ORGAN BOUNDARIES DOMAIN 4 (LBD4). PXY signalling in turn regulates the WOX14, TMO6, LBD4 loop to control vascular proliferation. Genetic interaction between LBD4 and PXY suggests that LBD4 marks the phloem-procambium boundary, thus defining the shape of the vascular bundle. These data collectively support a novel mechanism that influences recruitment of cells into the phloem lineage, and defines the role of PXY signalling in this context to the arrangement of vascular tissue.
Original languageEnglish
JournalThe Plant Cell
Volume32
Issue number2
DOIs
Publication statusPublished - Feb 2020

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Gene Regulatory Networks
Phloem
Arabidopsis
blood vessels
phloem
Blood Vessels
transcription factors
Xylem
Transcription Factors
xylem
vascular tissues
vascular bundles
meristems
Meristem
Homeobox Genes
stem cells
cell division
phosphotransferases (kinases)
Cell Division
Biomass

Cite this

Smit, Margot ; Mcgregor, Shauni ; Sun, Heng ; Gough, Catherine ; Bågman, Anne-Maarit ; Soyars, Cara L. ; Kroon, Johan T.M. ; Gaudinier, Allison ; Williams, Clara J. ; Yang, Xiyan ; Nimchuk, Zachary L. ; Weijers, Dolf ; Turner, Simon R. ; Brady, Siobhan M. ; Etchells, Peter. / A PXY-Mediated Transcriptional Network Integrates Signaling Mechanisms to Control Vascular Development in Arabidopsis. In: The Plant Cell. 2020 ; Vol. 32, No. 2.
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title = "A PXY-Mediated Transcriptional Network Integrates Signaling Mechanisms to Control Vascular Development in Arabidopsis",
abstract = "Vascular meristems generate the majority of biomass in higher plants. They constitute a bifacial stem cell population from which xylem and phloem are specified on opposing sides by positional signals. The PHLOEM INTERCALATED WITH XYLEM (PXY) receptor kinase promotes vascular cell division and organisation. However, how these functions are specified and integrated is unknown. Here, a putative PXY-mediated transcriptional regulatory network comprised of 690 transcription factor-promoter interactions was mapped. Among these interactions was a feed-forward loop containing transcription factors WUSCHEL HOMEOBOX RELATED 14 (WOX14) and TARGET OF MONOPTEROS 6 (TMO6), which each regulate the expression of a third transcription factor, LATERAL ORGAN BOUNDARIES DOMAIN 4 (LBD4). PXY signalling in turn regulates the WOX14, TMO6, LBD4 loop to control vascular proliferation. Genetic interaction between LBD4 and PXY suggests that LBD4 marks the phloem-procambium boundary, thus defining the shape of the vascular bundle. These data collectively support a novel mechanism that influences recruitment of cells into the phloem lineage, and defines the role of PXY signalling in this context to the arrangement of vascular tissue.",
author = "Margot Smit and Shauni Mcgregor and Heng Sun and Catherine Gough and Anne-Maarit B{\aa}gman and Soyars, {Cara L.} and Kroon, {Johan T.M.} and Allison Gaudinier and Williams, {Clara J.} and Xiyan Yang and Nimchuk, {Zachary L.} and Dolf Weijers and Turner, {Simon R.} and Brady, {Siobhan M.} and Peter Etchells",
year = "2020",
month = "2",
doi = "10.1105/tpc.19.00562",
language = "English",
volume = "32",
journal = "The Plant Cell",
issn = "1040-4651",
publisher = "American Society of Plant Biologists",
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Smit, M, Mcgregor, S, Sun, H, Gough, C, Bågman, A-M, Soyars, CL, Kroon, JTM, Gaudinier, A, Williams, CJ, Yang, X, Nimchuk, ZL, Weijers, D, Turner, SR, Brady, SM & Etchells, P 2020, 'A PXY-Mediated Transcriptional Network Integrates Signaling Mechanisms to Control Vascular Development in Arabidopsis', The Plant Cell, vol. 32, no. 2. https://doi.org/10.1105/tpc.19.00562

A PXY-Mediated Transcriptional Network Integrates Signaling Mechanisms to Control Vascular Development in Arabidopsis. / Smit, Margot; Mcgregor, Shauni; Sun, Heng; Gough, Catherine; Bågman, Anne-Maarit; Soyars, Cara L.; Kroon, Johan T.M.; Gaudinier, Allison; Williams, Clara J.; Yang, Xiyan; Nimchuk, Zachary L.; Weijers, Dolf; Turner, Simon R.; Brady, Siobhan M.; Etchells, Peter.

In: The Plant Cell, Vol. 32, No. 2, 02.2020.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - A PXY-Mediated Transcriptional Network Integrates Signaling Mechanisms to Control Vascular Development in Arabidopsis

AU - Smit, Margot

AU - Mcgregor, Shauni

AU - Sun, Heng

AU - Gough, Catherine

AU - Bågman, Anne-Maarit

AU - Soyars, Cara L.

AU - Kroon, Johan T.M.

AU - Gaudinier, Allison

AU - Williams, Clara J.

AU - Yang, Xiyan

AU - Nimchuk, Zachary L.

AU - Weijers, Dolf

AU - Turner, Simon R.

AU - Brady, Siobhan M.

AU - Etchells, Peter

PY - 2020/2

Y1 - 2020/2

N2 - Vascular meristems generate the majority of biomass in higher plants. They constitute a bifacial stem cell population from which xylem and phloem are specified on opposing sides by positional signals. The PHLOEM INTERCALATED WITH XYLEM (PXY) receptor kinase promotes vascular cell division and organisation. However, how these functions are specified and integrated is unknown. Here, a putative PXY-mediated transcriptional regulatory network comprised of 690 transcription factor-promoter interactions was mapped. Among these interactions was a feed-forward loop containing transcription factors WUSCHEL HOMEOBOX RELATED 14 (WOX14) and TARGET OF MONOPTEROS 6 (TMO6), which each regulate the expression of a third transcription factor, LATERAL ORGAN BOUNDARIES DOMAIN 4 (LBD4). PXY signalling in turn regulates the WOX14, TMO6, LBD4 loop to control vascular proliferation. Genetic interaction between LBD4 and PXY suggests that LBD4 marks the phloem-procambium boundary, thus defining the shape of the vascular bundle. These data collectively support a novel mechanism that influences recruitment of cells into the phloem lineage, and defines the role of PXY signalling in this context to the arrangement of vascular tissue.

AB - Vascular meristems generate the majority of biomass in higher plants. They constitute a bifacial stem cell population from which xylem and phloem are specified on opposing sides by positional signals. The PHLOEM INTERCALATED WITH XYLEM (PXY) receptor kinase promotes vascular cell division and organisation. However, how these functions are specified and integrated is unknown. Here, a putative PXY-mediated transcriptional regulatory network comprised of 690 transcription factor-promoter interactions was mapped. Among these interactions was a feed-forward loop containing transcription factors WUSCHEL HOMEOBOX RELATED 14 (WOX14) and TARGET OF MONOPTEROS 6 (TMO6), which each regulate the expression of a third transcription factor, LATERAL ORGAN BOUNDARIES DOMAIN 4 (LBD4). PXY signalling in turn regulates the WOX14, TMO6, LBD4 loop to control vascular proliferation. Genetic interaction between LBD4 and PXY suggests that LBD4 marks the phloem-procambium boundary, thus defining the shape of the vascular bundle. These data collectively support a novel mechanism that influences recruitment of cells into the phloem lineage, and defines the role of PXY signalling in this context to the arrangement of vascular tissue.

U2 - 10.1105/tpc.19.00562

DO - 10.1105/tpc.19.00562

M3 - Article

VL - 32

JO - The Plant Cell

JF - The Plant Cell

SN - 1040-4651

IS - 2

ER -