A preliminary amplified fragment length polymorphism (AFLP) primers selection for spathoglottis species

F.C. Ginibun, S. Bhassu, N. Khalid, P. Arens, B. Vosman, R.Y. Othman

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Spathoglottis is a native terrestrial orchid that attracting horticultural interest as a landscaping plant. Distinguishing orchid at species level based on morphological characteristics is unconvincing as some characteristic may sensitive to environmental. Therefore, molecular characterization could be an alternative method in characterizing Spathoglottis species. Amplified Fragment Length Polymorphism (AFLP) marker was selected to characterize the Spathoglottis species. Among 56 AFLP primer combinations tested on Spathoglottis plicata, only eight primer combinations gave clear scorable polymorphic bands. A total of 60 accessions of Spathoglottis species, hybrid and outgroup (Dendrobium) were analysed using the eight primer combinations and generated 891 polymorphic bands with 98.41% in average. The AFLP patterns showed that the Spathoglottis species sampled were clearly different.

Original languageEnglish
Pages (from-to)167-173
JournalActa Horticulturae
Volume1078
DOIs
Publication statusPublished - 2015
EventII International Orchid Symposium, Bangkok, Thailand -
Duration: 27 Mar 2015 → …

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Spathoglottis
amplified fragment length polymorphism
Dendrobium
landscaping

Keywords

  • AFLP primer
  • Molecular marker
  • Terrestrial orchid

Cite this

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abstract = "Spathoglottis is a native terrestrial orchid that attracting horticultural interest as a landscaping plant. Distinguishing orchid at species level based on morphological characteristics is unconvincing as some characteristic may sensitive to environmental. Therefore, molecular characterization could be an alternative method in characterizing Spathoglottis species. Amplified Fragment Length Polymorphism (AFLP) marker was selected to characterize the Spathoglottis species. Among 56 AFLP primer combinations tested on Spathoglottis plicata, only eight primer combinations gave clear scorable polymorphic bands. A total of 60 accessions of Spathoglottis species, hybrid and outgroup (Dendrobium) were analysed using the eight primer combinations and generated 891 polymorphic bands with 98.41{\%} in average. The AFLP patterns showed that the Spathoglottis species sampled were clearly different.",
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A preliminary amplified fragment length polymorphism (AFLP) primers selection for spathoglottis species. / Ginibun, F.C.; Bhassu, S.; Khalid, N.; Arens, P.; Vosman, B.; Othman, R.Y.

In: Acta Horticulturae, Vol. 1078, 2015, p. 167-173.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - A preliminary amplified fragment length polymorphism (AFLP) primers selection for spathoglottis species

AU - Ginibun, F.C.

AU - Bhassu, S.

AU - Khalid, N.

AU - Arens, P.

AU - Vosman, B.

AU - Othman, R.Y.

PY - 2015

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AB - Spathoglottis is a native terrestrial orchid that attracting horticultural interest as a landscaping plant. Distinguishing orchid at species level based on morphological characteristics is unconvincing as some characteristic may sensitive to environmental. Therefore, molecular characterization could be an alternative method in characterizing Spathoglottis species. Amplified Fragment Length Polymorphism (AFLP) marker was selected to characterize the Spathoglottis species. Among 56 AFLP primer combinations tested on Spathoglottis plicata, only eight primer combinations gave clear scorable polymorphic bands. A total of 60 accessions of Spathoglottis species, hybrid and outgroup (Dendrobium) were analysed using the eight primer combinations and generated 891 polymorphic bands with 98.41% in average. The AFLP patterns showed that the Spathoglottis species sampled were clearly different.

KW - AFLP primer

KW - Molecular marker

KW - Terrestrial orchid

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