A possible molecular basis for photoprotection in the minor antenna proteins of plants

Kieran F. Fox, Caner Ünlü, Vytautas Balevičius, Baboo Narottamsing Ramdour, Carina Kern, Xiaowei Pan, Mei Li, Herbert van Amerongen, Christopher D.P. Duffy*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

The bioenergetics of light-harvesting by photosynthetic antenna proteins in higher plants is well understood. However, investigation into the regulatory non-photochemical quenching (NPQ) mechanism, which dissipates excess energy in high light, has led to several conflicting models. It is generally accepted that the major photosystem II antenna protein, LHCII, is the site of NPQ, although the minor antenna complexes (CP24/26/29) are also proposed as alternative/additional NPQ sites. LHCII crystals were shown to exhibit the short excitation lifetime and several spectral signatures of the quenched state. Subsequent structure-based models showed that this quenching could be explained by slow energy trapping by the carotenoids, in line with one of the proposed models. Using Fluorescence Lifetime Imaging Microscopy (FLIM) we show that the crystal structure of CP29 corresponds to a strongly quenched conformation. Using a structure-based theoretical model we show that this quenching may be explained by the same slow, carotenoid-mediated quenching mechanism present in LHCII crystals.

Original languageEnglish
Pages (from-to)471-481
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1859
Issue number7
DOIs
Publication statusPublished - 1 Jul 2018

Keywords

  • Carotenoids
  • Light-harvesting
  • Minor antenna
  • Non-photochemical quenching
  • Photoprotection
  • Photosystem II

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    Fox, K. F., Ünlü, C., Balevičius, V., Ramdour, B. N., Kern, C., Pan, X., ... Duffy, C. D. P. (2018). A possible molecular basis for photoprotection in the minor antenna proteins of plants. Biochimica et Biophysica Acta - Bioenergetics, 1859(7), 471-481. https://doi.org/10.1016/j.bbabio.2018.03.015