A physiologically based kinetic model for bacterial sulfide oxidation

J.B.M. Klok, C.M. de Graaff, P.L.F. van den Bosch, N.C. Boelee, K.J. Keesman, A.J.H. Janssen

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29 Citations (Scopus)

Abstract

In the biotechnological process for hydrogen sulfide removal from gas streams, a variety of oxidation products can be formed. Under natron-alkaline conditions, sulfide is oxidized by haloalkaliphilic sulfide oxidizing bacteria via flavocytochrome c oxidoreductase. From previous studies, it was concluded that the oxidation-reduction state of cytochrome c is a direct measure for the bacterial end-product formation. Given this physiological feature, incorporation of the oxidation state of cytochrome c in a mathematical model for the bacterial oxidation kinetics will yield a physiologically based model structure. This paper presents a physiologically based model, describing the dynamic formation of the various end-products in the biodesulfurization process. It consists of three elements: 1) Michaelis–Menten kinetics combined with 2) a cytochrome c driven mechanism describing 3) the rate determining enzymes of the respiratory system of haloalkaliphilic sulfide oxidizing bacteria. The proposed model is successfully validated against independent data obtained from biological respiration tests and bench scale gas-lift reactor experiments. The results demonstrate that the model is a powerful tool to describe product formation for haloalkaliphilic biomass under dynamic conditions. The model predicts a maximum S0 formation of about 98 mol%. A future challenge is the optimization of this bioprocess by improving the dissolved oxygen control strategy and reactor design.
Original languageEnglish
Pages (from-to)483-492
JournalWater Research
Volume47
Issue number2
DOIs
Publication statusPublished - 2013

Keywords

  • waste water treatment
  • biotechnology
  • hydrogen sulfide
  • oxidation
  • desulfurization
  • alkalibacillus haloalkaliphilus
  • microbial physiology
  • waste water treatment plants
  • sulfur-oxidizing bacteria
  • biologically produced sulfur
  • dissolved sodium sulfide
  • parameter-estimation
  • hydrogen-sulfide
  • soda lakes
  • bioreactors
  • thiosulfate
  • mechanisms
  • pathways

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  • Cite this

    Klok, J. B. M., de Graaff, C. M., van den Bosch, P. L. F., Boelee, N. C., Keesman, K. J., & Janssen, A. J. H. (2013). A physiologically based kinetic model for bacterial sulfide oxidation. Water Research, 47(2), 483-492. https://doi.org/10.1016/j.watres.2012.09.021