Nucleotide-binding, leucine-rich repeat (NB-LRR) immune receptors from plants confer resistance to fungal pathogens and many other pathogenic organisms. Their low expression makes it challenging to purify these receptors from plants in sufficient quantities to be able to identify interacting proteins by mass spectrometry. Here we describe a protocol to affinity-purify recombinant NB-LRR immune receptors, fused to the streptavidin-binding peptide tag.
|Title of host publication||Plant Fungal Pathogens: Methods and Protocols|
|Editors||M.D. Bolton, B.P.H.J. Thomma|
|Place of Publication||New York, USA|
|Number of pages||648|
|Publication status||Published - 2012|
|Name||Methods in Molecular Biology|
|Publisher||Humana Press, c/o Springer Science+Business Media|