A one-step affinity-purification protocol to purify NB-LRR immune receptors from plants that mediate resistance to fungal pathogens

W.I.L. Tameling

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Nucleotide-binding, leucine-rich repeat (NB-LRR) immune receptors from plants confer resistance to fungal pathogens and many other pathogenic organisms. Their low expression makes it challenging to purify these receptors from plants in sufficient quantities to be able to identify interacting proteins by mass spectrometry. Here we describe a protocol to affinity-purify recombinant NB-LRR immune receptors, fused to the streptavidin-binding peptide tag.
Original languageEnglish
Title of host publicationPlant Fungal Pathogens: Methods and Protocols
EditorsM.D. Bolton, B.P.H.J. Thomma
Place of PublicationNew York, USA
Pages585-590
Number of pages648
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
PublisherHumana Press, c/o Springer Science+Business Media
Number835

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    Tameling, W. I. L. (2012). A one-step affinity-purification protocol to purify NB-LRR immune receptors from plants that mediate resistance to fungal pathogens. In M. D. Bolton, & B. P. H. J. Thomma (Eds.), Plant Fungal Pathogens: Methods and Protocols (pp. 585-590). (Methods in Molecular Biology; No. 835).. https://doi.org/10.1007/978-1-61779-501-5_36