A novel technique capable of taking ‘protected’ biopsies for reliable assessment of the distribution of microbiota along the colonic mucosa

Floortje Strobbe; Mèlanie V. Bénard; Noortje G. Rossen; Willem M. de Vos; Nitin Kumar; Trevor D. Lawley; Erwin G. Zoetendal; Floor Hugenholtz; Cyriel Y. Ponsioen

doi:10.1016/j.mimet.2021.106204

A novel technique capable of taking ‘protected’ biopsies for reliable assessment of the distribution of microbiota along the colonic mucosa

Floortje Strobbe, Mèlanie V. Bénard, Noortje G. Rossen, Willem M. de Vos, Nitin Kumar, Trevor D. Lawley, Erwin G. Zoetendal, Floor Hugenholtz, Cyriel Y. Ponsioen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

1 Citation (Scopus)

Abstract

We evaluated a novel ‘protected’ biopsy method to reliably ascertain the spatial distribution of the mucosa-adherent colonic microbiota. Apart from minor differences at genus level, overall similarities along the colon were high between the various areas, irrespective of protected or unprotected sampling.

Original language	English
Article number	106204
Journal	Journal of Microbiological Methods
Volume	185
Early online date	17 Apr 2021
DOIs	https://doi.org/10.1016/j.mimet.2021.106204
Publication status	Published - 2021

Keywords

Mucosa-adherent colonic microbiota
Protected biopsy technique

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Strobbe, F., Bénard, M. V., Rossen, N. G., de Vos, W. M., Kumar, N., Lawley, T. D., Zoetendal, E. G., Hugenholtz, F., & Ponsioen, C. Y. (2021). A novel technique capable of taking ‘protected’ biopsies for reliable assessment of the distribution of microbiota along the colonic mucosa. Journal of Microbiological Methods, 185, [106204]. https://doi.org/10.1016/j.mimet.2021.106204

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title = "A novel technique capable of taking {\textquoteleft}protected{\textquoteright} biopsies for reliable assessment of the distribution of microbiota along the colonic mucosa",

abstract = "We evaluated a novel {\textquoteleft}protected{\textquoteright} biopsy method to reliably ascertain the spatial distribution of the mucosa-adherent colonic microbiota. Apart from minor differences at genus level, overall similarities along the colon were high between the various areas, irrespective of protected or unprotected sampling.",

keywords = "Mucosa-adherent colonic microbiota, Protected biopsy technique",

author = "Floortje Strobbe and B{\'e}nard, {M{\`e}lanie V.} and Rossen, {Noortje G.} and {de Vos}, {Willem M.} and Nitin Kumar and Lawley, {Trevor D.} and Zoetendal, {Erwin G.} and Floor Hugenholtz and Ponsioen, {Cyriel Y.}",

year = "2021",

doi = "10.1016/j.mimet.2021.106204",

language = "English",

volume = "185",

journal = "Journal of Microbiological Methods",

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T1 - A novel technique capable of taking ‘protected’ biopsies for reliable assessment of the distribution of microbiota along the colonic mucosa

AU - Strobbe, Floortje

AU - Bénard, Mèlanie V.

AU - Rossen, Noortje G.

AU - de Vos, Willem M.

AU - Kumar, Nitin

AU - Lawley, Trevor D.

AU - Zoetendal, Erwin G.

AU - Hugenholtz, Floor

AU - Ponsioen, Cyriel Y.

PY - 2021

Y1 - 2021

N2 - We evaluated a novel ‘protected’ biopsy method to reliably ascertain the spatial distribution of the mucosa-adherent colonic microbiota. Apart from minor differences at genus level, overall similarities along the colon were high between the various areas, irrespective of protected or unprotected sampling.

AB - We evaluated a novel ‘protected’ biopsy method to reliably ascertain the spatial distribution of the mucosa-adherent colonic microbiota. Apart from minor differences at genus level, overall similarities along the colon were high between the various areas, irrespective of protected or unprotected sampling.

KW - Mucosa-adherent colonic microbiota

KW - Protected biopsy technique

U2 - 10.1016/j.mimet.2021.106204

DO - 10.1016/j.mimet.2021.106204

M3 - Article

C2 - 33872639

AN - SCOPUS:85105322670

VL - 185

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

M1 - 106204

ER -

A novel technique capable of taking ‘protected’ biopsies for reliable assessment of the distribution of microbiota along the colonic mucosa

Abstract

Keywords

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