A novel recombinant virus-like particle vaccine for prevention of porcine parvovirus-induced reproductive failure

A.F.G. Antonis, C.J.M. Bruschke, P. Rueda, L. Maranga, J. Casal, C. Vela, L.A.T. Hilgers, P.B.G.M. Belt, K. Weerdmeester, M.J. Carrondo, J.P.M. Langeveld

    Research output: Contribution to journalArticleAcademicpeer-review

    68 Citations (Scopus)

    Abstract

    A novel vaccine against porcine parvovirus (PPV), composed of recombinant virus-like particles (PPV-VLPs) produced with the baculovirus expression vector system (BEVS) at industrial scale, was tested for its immunogenicity and protective potency. A formulation of submicrogram amounts of PPV-VLPs in a water-in-mineral oil adjuvant evoked high serum antibody titres in both guinea pigs, used as reference model, and target species, pigs. A single immunisation with 0.7 ¿g of this antigen yielded complete foetal protection against PPV infection after challenge with a virulent strain of this virus. Furthermore, also in the presence of mild adjuvants the protective action of these PPV-VLPs is excellent. This recombinant subunit vaccine overcomes some of the drawbacks of classical PPV vaccines
    Original languageEnglish
    Pages (from-to)5481-5490
    JournalVaccine
    Volume24
    Issue number26
    DOIs
    Publication statusPublished - 2006

    Keywords

    • immune-responses
    • experimental-infection
    • baculovirus vectors
    • canine parvovirus
    • pregnant gilts
    • disease
    • pigs
    • coinfection
    • volunteers
    • adjuvants

    Fingerprint Dive into the research topics of 'A novel recombinant virus-like particle vaccine for prevention of porcine parvovirus-induced reproductive failure'. Together they form a unique fingerprint.

    Cite this