A novel recombinant virus-like particle vaccine for prevention of porcine parvovirus-induced reproductive failure

A.F.G. Antonis; C.J.M. Bruschke; P. Rueda; L. Maranga; J. Casal; C. Vela; L.A.T. Hilgers; P.B.G.M. Belt; K. Weerdmeester; M.J. Carrondo; J.P.M. Langeveld

doi:10.1016/j.vaccine.2006.03.089

A novel recombinant virus-like particle vaccine for prevention of porcine parvovirus-induced reproductive failure

A.F.G. Antonis, C.J.M. Bruschke, P. Rueda, L. Maranga, J. Casal, C. Vela, L.A.T. Hilgers, P.B.G.M. Belt, K. Weerdmeester, M.J. Carrondo, J.P.M. Langeveld

Research output: Contribution to journal › Article › Academic › peer-review

68 Citations (Scopus)

Abstract

A novel vaccine against porcine parvovirus (PPV), composed of recombinant virus-like particles (PPV-VLPs) produced with the baculovirus expression vector system (BEVS) at industrial scale, was tested for its immunogenicity and protective potency. A formulation of submicrogram amounts of PPV-VLPs in a water-in-mineral oil adjuvant evoked high serum antibody titres in both guinea pigs, used as reference model, and target species, pigs. A single immunisation with 0.7 ¿g of this antigen yielded complete foetal protection against PPV infection after challenge with a virulent strain of this virus. Furthermore, also in the presence of mild adjuvants the protective action of these PPV-VLPs is excellent. This recombinant subunit vaccine overcomes some of the drawbacks of classical PPV vaccines

Original language	English
Pages (from-to)	5481-5490
Journal	Vaccine
Volume	24
Issue number	26
DOIs	https://doi.org/10.1016/j.vaccine.2006.03.089
Publication status	Published - 2006

Keywords

immune-responses
experimental-infection
baculovirus vectors
canine parvovirus
pregnant gilts
disease
pigs
coinfection
volunteers
adjuvants

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Antonis, A. F. G., Bruschke, C. J. M., Rueda, P., Maranga, L., Casal, J., Vela, C., Hilgers, L. A. T., Belt, P. B. G. M., Weerdmeester, K., Carrondo, M. J., & Langeveld, J. P. M. (2006). A novel recombinant virus-like particle vaccine for prevention of porcine parvovirus-induced reproductive failure. Vaccine, 24(26), 5481-5490. https://doi.org/10.1016/j.vaccine.2006.03.089

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abstract = "A novel vaccine against porcine parvovirus (PPV), composed of recombinant virus-like particles (PPV-VLPs) produced with the baculovirus expression vector system (BEVS) at industrial scale, was tested for its immunogenicity and protective potency. A formulation of submicrogram amounts of PPV-VLPs in a water-in-mineral oil adjuvant evoked high serum antibody titres in both guinea pigs, used as reference model, and target species, pigs. A single immunisation with 0.7 ¿g of this antigen yielded complete foetal protection against PPV infection after challenge with a virulent strain of this virus. Furthermore, also in the presence of mild adjuvants the protective action of these PPV-VLPs is excellent. This recombinant subunit vaccine overcomes some of the drawbacks of classical PPV vaccines",

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A novel recombinant virus-like particle vaccine for prevention of porcine parvovirus-induced reproductive failure. / Antonis, A.F.G.; Bruschke, C.J.M.; Rueda, P.; Maranga, L.; Casal, J.; Vela, C.; Hilgers, L.A.T.; Belt, P.B.G.M.; Weerdmeester, K.; Carrondo, M.J.; Langeveld, J.P.M.

In: Vaccine, Vol. 24, No. 26, 2006, p. 5481-5490.

Research output: Contribution to journal › Article › Academic › peer-review

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AU - Maranga, L.

AU - Casal, J.

AU - Vela, C.

AU - Hilgers, L.A.T.

AU - Belt, P.B.G.M.

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AU - Langeveld, J.P.M.

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AB - A novel vaccine against porcine parvovirus (PPV), composed of recombinant virus-like particles (PPV-VLPs) produced with the baculovirus expression vector system (BEVS) at industrial scale, was tested for its immunogenicity and protective potency. A formulation of submicrogram amounts of PPV-VLPs in a water-in-mineral oil adjuvant evoked high serum antibody titres in both guinea pigs, used as reference model, and target species, pigs. A single immunisation with 0.7 ¿g of this antigen yielded complete foetal protection against PPV infection after challenge with a virulent strain of this virus. Furthermore, also in the presence of mild adjuvants the protective action of these PPV-VLPs is excellent. This recombinant subunit vaccine overcomes some of the drawbacks of classical PPV vaccines

KW - immune-responses

KW - experimental-infection

KW - baculovirus vectors

KW - canine parvovirus

KW - pregnant gilts

KW - disease

KW - pigs

KW - coinfection

KW - volunteers

KW - adjuvants

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