A novel highly sensitive, rapid and safe Rift Valley fever virus neutralization test

Paul Wichgers Schreur, Janusz T. Paweska, Jet Kant-Eenbergen, Jeroen Kortekaas

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Antibodies specific for Rift Valley fever virus (RVFV) can be detected by diverse methods, including ezyme-linked immunosortbent assay (ELISA) and virus neutralization test (VNT). The VNT is superior in sensitivity and specificity and is therefore considered the gold standard serological assay. Classical VNTs make use of virulent RVFV and therefore have to be performed in biosafety level 3 laboratories. Here, we report the development of a novel VNT that is based on an avirulent RVFV expressing the enhanced green fluorescent protein (eGFP), which can be performed safely outside level 3 biocontainment facilities. Evaluation with a broad panel of experimental sera and field sera demonstrated that this novel VNT is faster and more sensitive than the classical VNT.

LanguageEnglish
Pages26-30
JournalJournal of Virological Methods
Volume248
DOIs
Publication statusPublished - 2017

Fingerprint

Rift Valley fever virus
Neutralization Tests
Viruses
Serum
Sensitivity and Specificity
Antibodies

Keywords

  • 4-Segmented
  • GFP
  • Rift Valley fever virus
  • Ruminants
  • Virus neutralization test

Cite this

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title = "A novel highly sensitive, rapid and safe Rift Valley fever virus neutralization test",
abstract = "Antibodies specific for Rift Valley fever virus (RVFV) can be detected by diverse methods, including ezyme-linked immunosortbent assay (ELISA) and virus neutralization test (VNT). The VNT is superior in sensitivity and specificity and is therefore considered the gold standard serological assay. Classical VNTs make use of virulent RVFV and therefore have to be performed in biosafety level 3 laboratories. Here, we report the development of a novel VNT that is based on an avirulent RVFV expressing the enhanced green fluorescent protein (eGFP), which can be performed safely outside level 3 biocontainment facilities. Evaluation with a broad panel of experimental sera and field sera demonstrated that this novel VNT is faster and more sensitive than the classical VNT.",
keywords = "4-Segmented, GFP, Rift Valley fever virus, Ruminants, Virus neutralization test",
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A novel highly sensitive, rapid and safe Rift Valley fever virus neutralization test. / Wichgers Schreur, Paul; Paweska, Janusz T.; Kant-Eenbergen, Jet; Kortekaas, Jeroen.

In: Journal of Virological Methods, Vol. 248, 2017, p. 26-30.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Paweska, Janusz T.

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AB - Antibodies specific for Rift Valley fever virus (RVFV) can be detected by diverse methods, including ezyme-linked immunosortbent assay (ELISA) and virus neutralization test (VNT). The VNT is superior in sensitivity and specificity and is therefore considered the gold standard serological assay. Classical VNTs make use of virulent RVFV and therefore have to be performed in biosafety level 3 laboratories. Here, we report the development of a novel VNT that is based on an avirulent RVFV expressing the enhanced green fluorescent protein (eGFP), which can be performed safely outside level 3 biocontainment facilities. Evaluation with a broad panel of experimental sera and field sera demonstrated that this novel VNT is faster and more sensitive than the classical VNT.

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