A novel Cry9Aa with increased toxicity for Spodoptera exigua (Hübner)

S. Naimov, R. Nedyalkova, N. Staykov, M. Weemen-Hendriks, I. Minkov, R.A. de Maagd

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)

Abstract

Cry9Aa, produced by Bacillus thuringiensis is reported to be not active against Spodoptera exigua (beet armyworm). In this study we have cloned a new cry9Aa5 gene encoding a protoxin with increased activity against S. exigua as compared to Cry9Aa1. When aligned to Cry9Aa1, four amino acid substitutions in domain I and one substitution in the C-terminal protein extension of Cry9Aa5 were identified. Toxicity of Cry9Aa5, produced in recombinant Escherichia coli was assessed and compared to the activity of Cry9Aa1, produced under the same conditions.
Original languageEnglish
Pages (from-to)99-101
JournalJournal of Invertebrate Pathology
Volume115
DOIs
Publication statusPublished - 2014

Keywords

  • bacillus-thuringiensis
  • crystal proteins
  • domain-iii
  • specificity
  • resistance
  • noctuidae
  • larvae

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