A novel Aux/IAA28 signalling cascade activates GATA23-dependent specification of lateral root founder cell identity

B. De Rybel; V. Vassileva; B. Parizot; M. Demeulenaere; W. Grunewald; D. Audenaert; J. Van Campenhout; P. Overvoorde; L. Janssen; S. Vanneste; B.K. Moller; M. Wilson; T. Holman; G. Van Isterdaele; G. Brunoud; M. Vuylsteke; T. Vernoux; L. De Veylder; D. Inze; D. Weijers; M. Bennett; T. Beeckman

doi:10.1016/j.cub.2010.09.007

A novel Aux/IAA28 signalling cascade activates GATA23-dependent specification of lateral root founder cell identity

B. De Rybel, V. Vassileva, B. Parizot, M. Demeulenaere, W. Grunewald, D. Audenaert, J. Van Campenhout, P. Overvoorde, L. Janssen, S. Vanneste, B.K. Moller, M. Wilson, T. Holman, G. Van Isterdaele, G. Brunoud, M. Vuylsteke, T. Vernoux, L. De Veylder, D. Inze, D. WeijersM. Bennett, T. Beeckman

Biochemistry

Research output: Contribution to journal › Article › Academic › peer-review

390 Citations (Scopus)

Abstract

Background - Lateral roots are formed at regular intervals along the main root by recurrent specification of founder cells. To date, the mechanism by which branching of the root system is controlled and founder cells become specified remains unknown. Results - Our study reports the identification of the auxin regulatory components and their target gene, GATA23, which control lateral root founder cell specification. Initially, a meta-analysis of lateral root-related transcriptomic data identified the GATA23 transcription factor. GATA23 is expressed specifically in xylem pole pericycle cells before the first asymmetric division and is correlated with oscillating auxin signaling maxima in the basal meristem. Also, functional studies revealed that GATA23 controls lateral root founder cell identity. Finally, we show that an Aux/IAA28-dependent auxin signaling mechanism in the basal meristem controls GATA23 expression. Conclusions - We have identified the first molecular components that control lateral root founder cell identity in the Arabidopsis root. These include an IAA28-dependent auxin signaling module in the basal meristem region that regulates GATA23 expression and thereby lateral root founder cell specification and root branching patterns

Original language	English
Pages (from-to)	1697-1706
Journal	Current Biology
Volume	20
Issue number	19
DOIs	https://doi.org/10.1016/j.cub.2010.09.007
Publication status	Published - 2010

Keywords

of-function mutation
arabidopsis-thaliana
auxin transport
initiation
gene
fate
differentiation
expression
pericycle
divisions

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De Rybel, B., Vassileva, V., Parizot, B., Demeulenaere, M., Grunewald, W., Audenaert, D., Van Campenhout, J., Overvoorde, P., Janssen, L., Vanneste, S., Moller, B. K., Wilson, M., Holman, T., Van Isterdaele, G., Brunoud, G., Vuylsteke, M., Vernoux, T., De Veylder, L., Inze, D., ... Beeckman, T. (2010). A novel Aux/IAA28 signalling cascade activates GATA23-dependent specification of lateral root founder cell identity. Current Biology, 20(19), 1697-1706. https://doi.org/10.1016/j.cub.2010.09.007

@article{45a5a49bcf744741b0deae34123fa973,

title = "A novel Aux/IAA28 signalling cascade activates GATA23-dependent specification of lateral root founder cell identity",

abstract = "Background - Lateral roots are formed at regular intervals along the main root by recurrent specification of founder cells. To date, the mechanism by which branching of the root system is controlled and founder cells become specified remains unknown. Results - Our study reports the identification of the auxin regulatory components and their target gene, GATA23, which control lateral root founder cell specification. Initially, a meta-analysis of lateral root-related transcriptomic data identified the GATA23 transcription factor. GATA23 is expressed specifically in xylem pole pericycle cells before the first asymmetric division and is correlated with oscillating auxin signaling maxima in the basal meristem. Also, functional studies revealed that GATA23 controls lateral root founder cell identity. Finally, we show that an Aux/IAA28-dependent auxin signaling mechanism in the basal meristem controls GATA23 expression. Conclusions - We have identified the first molecular components that control lateral root founder cell identity in the Arabidopsis root. These include an IAA28-dependent auxin signaling module in the basal meristem region that regulates GATA23 expression and thereby lateral root founder cell specification and root branching patterns",

keywords = "of-function mutation, arabidopsis-thaliana, auxin transport, initiation, gene, fate, differentiation, expression, pericycle, divisions",

author = "{De Rybel}, B. and V. Vassileva and B. Parizot and M. Demeulenaere and W. Grunewald and D. Audenaert and {Van Campenhout}, J. and P. Overvoorde and L. Janssen and S. Vanneste and B.K. Moller and M. Wilson and T. Holman and {Van Isterdaele}, G. and G. Brunoud and M. Vuylsteke and T. Vernoux and {De Veylder}, L. and D. Inze and D. Weijers and M. Bennett and T. Beeckman",

year = "2010",

doi = "10.1016/j.cub.2010.09.007",

language = "English",

volume = "20",

pages = "1697--1706",

journal = "Current Biology",

issn = "0960-9822",

publisher = "Elsevier",

number = "19",

}

De Rybel, B, Vassileva, V, Parizot, B, Demeulenaere, M, Grunewald, W, Audenaert, D, Van Campenhout, J, Overvoorde, P, Janssen, L, Vanneste, S, Moller, BK, Wilson, M, Holman, T, Van Isterdaele, G, Brunoud, G, Vuylsteke, M, Vernoux, T, De Veylder, L, Inze, D, Weijers, D, Bennett, M & Beeckman, T 2010, 'A novel Aux/IAA28 signalling cascade activates GATA23-dependent specification of lateral root founder cell identity', Current Biology, vol. 20, no. 19, pp. 1697-1706. https://doi.org/10.1016/j.cub.2010.09.007

TY - JOUR

T1 - A novel Aux/IAA28 signalling cascade activates GATA23-dependent specification of lateral root founder cell identity

AU - De Rybel, B.

AU - Vassileva, V.

AU - Parizot, B.

AU - Demeulenaere, M.

AU - Grunewald, W.

AU - Audenaert, D.

AU - Van Campenhout, J.

AU - Overvoorde, P.

AU - Janssen, L.

AU - Vanneste, S.

AU - Moller, B.K.

AU - Wilson, M.

AU - Holman, T.

AU - Van Isterdaele, G.

AU - Brunoud, G.

AU - Vuylsteke, M.

AU - Vernoux, T.

AU - De Veylder, L.

AU - Inze, D.

AU - Weijers, D.

AU - Bennett, M.

AU - Beeckman, T.

PY - 2010

Y1 - 2010

N2 - Background - Lateral roots are formed at regular intervals along the main root by recurrent specification of founder cells. To date, the mechanism by which branching of the root system is controlled and founder cells become specified remains unknown. Results - Our study reports the identification of the auxin regulatory components and their target gene, GATA23, which control lateral root founder cell specification. Initially, a meta-analysis of lateral root-related transcriptomic data identified the GATA23 transcription factor. GATA23 is expressed specifically in xylem pole pericycle cells before the first asymmetric division and is correlated with oscillating auxin signaling maxima in the basal meristem. Also, functional studies revealed that GATA23 controls lateral root founder cell identity. Finally, we show that an Aux/IAA28-dependent auxin signaling mechanism in the basal meristem controls GATA23 expression. Conclusions - We have identified the first molecular components that control lateral root founder cell identity in the Arabidopsis root. These include an IAA28-dependent auxin signaling module in the basal meristem region that regulates GATA23 expression and thereby lateral root founder cell specification and root branching patterns

AB - Background - Lateral roots are formed at regular intervals along the main root by recurrent specification of founder cells. To date, the mechanism by which branching of the root system is controlled and founder cells become specified remains unknown. Results - Our study reports the identification of the auxin regulatory components and their target gene, GATA23, which control lateral root founder cell specification. Initially, a meta-analysis of lateral root-related transcriptomic data identified the GATA23 transcription factor. GATA23 is expressed specifically in xylem pole pericycle cells before the first asymmetric division and is correlated with oscillating auxin signaling maxima in the basal meristem. Also, functional studies revealed that GATA23 controls lateral root founder cell identity. Finally, we show that an Aux/IAA28-dependent auxin signaling mechanism in the basal meristem controls GATA23 expression. Conclusions - We have identified the first molecular components that control lateral root founder cell identity in the Arabidopsis root. These include an IAA28-dependent auxin signaling module in the basal meristem region that regulates GATA23 expression and thereby lateral root founder cell specification and root branching patterns

KW - of-function mutation

KW - arabidopsis-thaliana

KW - auxin transport

KW - initiation

KW - gene

KW - fate

KW - differentiation

KW - expression

KW - pericycle

KW - divisions

U2 - 10.1016/j.cub.2010.09.007

DO - 10.1016/j.cub.2010.09.007

M3 - Article

SN - 0960-9822

VL - 20

SP - 1697

EP - 1706

JO - Current Biology

JF - Current Biology

IS - 19

ER -

A novel Aux/IAA28 signalling cascade activates GATA23-dependent specification of lateral root founder cell identity

Abstract

Keywords

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