A novel activating chicken IgY FcR is related to leukocyte receptor complex (LRC)

B.C. Viertlboeck, R. Schmitt, M.A. Hanczaruk, R.P.M.A. Crooijmans, M.A.M. Groenen, T.W. Gobel

Research output: Contribution to journalArticleAcademicpeer-review

31 Citations (Scopus)

Abstract

FcRs have multifaceted roles in the immune system. Chicken FcRs were demonstrated on macrophages decades ago; however, only recently the chicken Ig-like receptor AB1, encoded in the leukocyte receptor complex, was molecularly identified as a high-affinity FcR. The present study was initiated to identify additional receptors with the capability to bind chicken immunoglobulins. Based on database searches, we cloned a novel chicken FcR, designated gallus gallus FcR (ggFcR), which was shown to bind selectively chicken IgY. The receptor consists of four extracellular C2-set Ig domains, followed by a transmembrane region containing arginine as a positively charged amino acid and a short cytoplasmic tail. ggFcR associates with the common -chain, indicative for an activating receptor, and real-time RT-PCR revealed high expression on PBMC, thrombocytes, and macrophages. The genomic organization is similar to most Ig-like receptor genes, where each Ig domain is encoded by a separate exon. Additionally, the ggFcR signal peptide is encoded by two exons, the second of which is 36 bp, a hallmark for genes encoded in the leukocyte receptor complex. Phylogenetic analysis also showed a relationship to genes encoded in the leukocyte receptor complex. Surprisingly, ggFcR is not encoded in the leukocyte receptor complex, but it is located as a single isolated gene at the extremity of chicken chromosome 20
Original languageEnglish
Pages (from-to)1533-1540
JournalThe Journal of Immunology
Volume182
Issue number3
DOIs
Publication statusPublished - 2009

Keywords

  • family
  • thrombocytes
  • diversification
  • expression
  • prediction
  • clearance
  • evolution
  • platelets
  • system
  • cells

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