A new virus infecting Myzus persicae has a genome organization similar to the species of the genus Densovirus

M. van Munster, A.M. Dullemans, M. Verbeek, J.F.J.M. van den Heuvel, C. Reinbold, V. Brault, A. Clérivet, F. van der Wilk

    Research output: Contribution to journalArticleAcademicpeer-review

    24 Citations (Scopus)

    Abstract

    The genomic sequence of a new icosahedral DNA virus infecting Myzus persicae has been determined. Analysis of 5499 nt of the viral genome revealed five open reading frames (ORFs) evenly distributed in the 5' half of both DNA strands. Three ORFs (ORF1-3) share the same strand, while two other ORFs (ORF4 and ORF5) are detected in the complementary sequence. The overall genomic organization is similar to that of species from the genus Densovirus. ORFs 1-3 most likely encode the non-structural proteins, since their putative products contain conserved replication motifs, NTP-binding domains and helicase domains similar to those found in the NS-1 protein of parvoviruses. The deduced amino acid sequences from ORFs 4 and 5 show sequence similarities with the structural proteins of the members of the genus Densovirus. These data indicate that this virus is a new species of the genus Densovirus in the family Parvoviridae. The virus was tentatively named Myzus persicae densovirus. The nucleotide sequence reported in this study appears in the EMBL, GenBank and DDBJ nucleotide sequence databases under accession number AY148187.
    Original languageEnglish
    Pages (from-to)165-172
    JournalJournal of General Virology
    Volume84
    DOIs
    Publication statusPublished - 2003

    Fingerprint

    Densovirus
    Aphids
    Open Reading Frames
    Genome
    Viruses
    Nucleic Acid Databases
    Parvoviridae
    Parvovirus
    Proteins
    DNA Viruses
    Viral Genome
    Amino Acid Sequence
    Databases
    DNA

    Keywords

    • complete nucleotide-sequence
    • periplaneta-fuliginosa
    • densonucleosis virus
    • parvoviruses
    • replication
    • expression
    • initiator
    • dna
    • transcription
    • alignment

    Cite this

    van Munster, M. ; Dullemans, A.M. ; Verbeek, M. ; van den Heuvel, J.F.J.M. ; Reinbold, C. ; Brault, V. ; Clérivet, A. ; van der Wilk, F. / A new virus infecting Myzus persicae has a genome organization similar to the species of the genus Densovirus. In: Journal of General Virology. 2003 ; Vol. 84. pp. 165-172.
    @article{016c97c9193d4d079ec674f679ff3dda,
    title = "A new virus infecting Myzus persicae has a genome organization similar to the species of the genus Densovirus",
    abstract = "The genomic sequence of a new icosahedral DNA virus infecting Myzus persicae has been determined. Analysis of 5499 nt of the viral genome revealed five open reading frames (ORFs) evenly distributed in the 5' half of both DNA strands. Three ORFs (ORF1-3) share the same strand, while two other ORFs (ORF4 and ORF5) are detected in the complementary sequence. The overall genomic organization is similar to that of species from the genus Densovirus. ORFs 1-3 most likely encode the non-structural proteins, since their putative products contain conserved replication motifs, NTP-binding domains and helicase domains similar to those found in the NS-1 protein of parvoviruses. The deduced amino acid sequences from ORFs 4 and 5 show sequence similarities with the structural proteins of the members of the genus Densovirus. These data indicate that this virus is a new species of the genus Densovirus in the family Parvoviridae. The virus was tentatively named Myzus persicae densovirus. The nucleotide sequence reported in this study appears in the EMBL, GenBank and DDBJ nucleotide sequence databases under accession number AY148187.",
    keywords = "complete nucleotide-sequence, periplaneta-fuliginosa, densonucleosis virus, parvoviruses, replication, expression, initiator, dna, transcription, alignment",
    author = "{van Munster}, M. and A.M. Dullemans and M. Verbeek and {van den Heuvel}, J.F.J.M. and C. Reinbold and V. Brault and A. Cl{\'e}rivet and {van der Wilk}, F.",
    year = "2003",
    doi = "10.1099/vir.0.18650-0",
    language = "English",
    volume = "84",
    pages = "165--172",
    journal = "Journal of General Virology",
    issn = "0022-1317",
    publisher = "Microbiology Society",

    }

    A new virus infecting Myzus persicae has a genome organization similar to the species of the genus Densovirus. / van Munster, M.; Dullemans, A.M.; Verbeek, M.; van den Heuvel, J.F.J.M.; Reinbold, C.; Brault, V.; Clérivet, A.; van der Wilk, F.

    In: Journal of General Virology, Vol. 84, 2003, p. 165-172.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - A new virus infecting Myzus persicae has a genome organization similar to the species of the genus Densovirus

    AU - van Munster, M.

    AU - Dullemans, A.M.

    AU - Verbeek, M.

    AU - van den Heuvel, J.F.J.M.

    AU - Reinbold, C.

    AU - Brault, V.

    AU - Clérivet, A.

    AU - van der Wilk, F.

    PY - 2003

    Y1 - 2003

    N2 - The genomic sequence of a new icosahedral DNA virus infecting Myzus persicae has been determined. Analysis of 5499 nt of the viral genome revealed five open reading frames (ORFs) evenly distributed in the 5' half of both DNA strands. Three ORFs (ORF1-3) share the same strand, while two other ORFs (ORF4 and ORF5) are detected in the complementary sequence. The overall genomic organization is similar to that of species from the genus Densovirus. ORFs 1-3 most likely encode the non-structural proteins, since their putative products contain conserved replication motifs, NTP-binding domains and helicase domains similar to those found in the NS-1 protein of parvoviruses. The deduced amino acid sequences from ORFs 4 and 5 show sequence similarities with the structural proteins of the members of the genus Densovirus. These data indicate that this virus is a new species of the genus Densovirus in the family Parvoviridae. The virus was tentatively named Myzus persicae densovirus. The nucleotide sequence reported in this study appears in the EMBL, GenBank and DDBJ nucleotide sequence databases under accession number AY148187.

    AB - The genomic sequence of a new icosahedral DNA virus infecting Myzus persicae has been determined. Analysis of 5499 nt of the viral genome revealed five open reading frames (ORFs) evenly distributed in the 5' half of both DNA strands. Three ORFs (ORF1-3) share the same strand, while two other ORFs (ORF4 and ORF5) are detected in the complementary sequence. The overall genomic organization is similar to that of species from the genus Densovirus. ORFs 1-3 most likely encode the non-structural proteins, since their putative products contain conserved replication motifs, NTP-binding domains and helicase domains similar to those found in the NS-1 protein of parvoviruses. The deduced amino acid sequences from ORFs 4 and 5 show sequence similarities with the structural proteins of the members of the genus Densovirus. These data indicate that this virus is a new species of the genus Densovirus in the family Parvoviridae. The virus was tentatively named Myzus persicae densovirus. The nucleotide sequence reported in this study appears in the EMBL, GenBank and DDBJ nucleotide sequence databases under accession number AY148187.

    KW - complete nucleotide-sequence

    KW - periplaneta-fuliginosa

    KW - densonucleosis virus

    KW - parvoviruses

    KW - replication

    KW - expression

    KW - initiator

    KW - dna

    KW - transcription

    KW - alignment

    U2 - 10.1099/vir.0.18650-0

    DO - 10.1099/vir.0.18650-0

    M3 - Article

    VL - 84

    SP - 165

    EP - 172

    JO - Journal of General Virology

    JF - Journal of General Virology

    SN - 0022-1317

    ER -