A new syringopeptin produced by bean strains of Pseudomonas syringae pv. syringae

Ingeborg Grgurina; Feliciana Mariotti; Vincenzo Fogliano; Monica Gallo; Andrea Scaloni; Nicola S. Iacobellis; Pietro Lo Cantore; Luisa Mannina; Valeria Van Axel Castelli; Maria Luigia Greco; Antonio Graniti

doi:10.1016/S0167-4838(02)00283-2

A new syringopeptin produced by bean strains of Pseudomonas syringae pv. syringae

Ingeborg Grgurina^*, Feliciana Mariotti, Vincenzo Fogliano, Monica Gallo, Andrea Scaloni, Nicola S. Iacobellis, Pietro Lo Cantore, Luisa Mannina, Valeria Van Axel Castelli, Maria Luigia Greco, Antonio Graniti

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

26 Citations (Scopus)

Abstract

Two strains (B728a and Y37) of the phytopathogenic bacterium Pseudomonas syringae pv. syringae isolated from bean (Phaseolus vulgaris) plants were shown to produce in culture both syringomycin, a lipodepsinonapeptide secreted by the majority of the strains of the bacterium, and a new form of syringopeptin, SP₂₂Phv. The structure of the latter metabolite was elucidated by the combined use of mass spectrometry (MS), nuclear magnetic resonance (NMR) spectroscopy and chemical procedures. Comparative phytotoxic and antimicrobial assays showed that SP₂₂Phv did not differ substantially from the previously characterized syringopeptin 22 (SP₂₂) as far as toxicity to plants was concerned, but was less active in inhibiting the growth of the test fungi Rhodotorula pilimanae and Geotrichum candidum and of the Gram-positive bacterium Bacillus megaterium.

Original language	English
Pages (from-to)	81-89
Number of pages	9
Journal	Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology
Volume	1597
Issue number	1
DOIs	https://doi.org/10.1016/S0167-4838(02)00283-2
Publication status	Published - 20 May 2002
Externally published	Yes

Keywords

Lipodepsipeptide
Phytotoxin
Pseudomonas syringae pv. syringae
Structure
Syringopeptin 22 Phv

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Grgurina, I., Mariotti, F., Fogliano, V., Gallo, M., Scaloni, A., Iacobellis, N. S., Lo Cantore, P., Mannina, L., Van Axel Castelli, V., Greco, M. L., & Graniti, A. (2002). A new syringopeptin produced by bean strains of Pseudomonas syringae pv. syringae. Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology, 1597(1), 81-89. https://doi.org/10.1016/S0167-4838(02)00283-2

Grgurina, Ingeborg ; Mariotti, Feliciana ; Fogliano, Vincenzo ; Gallo, Monica ; Scaloni, Andrea ; Iacobellis, Nicola S. ; Lo Cantore, Pietro ; Mannina, Luisa ; Van Axel Castelli, Valeria ; Greco, Maria Luigia ; Graniti, Antonio. / A new syringopeptin produced by bean strains of Pseudomonas syringae pv. syringae. In: Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology. 2002 ; Vol. 1597, No. 1. pp. 81-89.

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title = "A new syringopeptin produced by bean strains of Pseudomonas syringae pv. syringae",

abstract = "Two strains (B728a and Y37) of the phytopathogenic bacterium Pseudomonas syringae pv. syringae isolated from bean (Phaseolus vulgaris) plants were shown to produce in culture both syringomycin, a lipodepsinonapeptide secreted by the majority of the strains of the bacterium, and a new form of syringopeptin, SP22Phv. The structure of the latter metabolite was elucidated by the combined use of mass spectrometry (MS), nuclear magnetic resonance (NMR) spectroscopy and chemical procedures. Comparative phytotoxic and antimicrobial assays showed that SP22Phv did not differ substantially from the previously characterized syringopeptin 22 (SP22) as far as toxicity to plants was concerned, but was less active in inhibiting the growth of the test fungi Rhodotorula pilimanae and Geotrichum candidum and of the Gram-positive bacterium Bacillus megaterium.",

keywords = "Lipodepsipeptide, Phytotoxin, Pseudomonas syringae pv. syringae, Structure, Syringopeptin 22 Phv",

author = "Ingeborg Grgurina and Feliciana Mariotti and Vincenzo Fogliano and Monica Gallo and Andrea Scaloni and Iacobellis, {Nicola S.} and {Lo Cantore}, Pietro and Luisa Mannina and {Van Axel Castelli}, Valeria and Greco, {Maria Luigia} and Antonio Graniti",

year = "2002",

month = may,

day = "20",

doi = "10.1016/S0167-4838(02)00283-2",

language = "English",

volume = "1597",

pages = "81--89",

journal = "Biochimica et biophysica acta-protein structure and molecular enzymology",

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Grgurina, I, Mariotti, F, Fogliano, V, Gallo, M, Scaloni, A, Iacobellis, NS, Lo Cantore, P, Mannina, L, Van Axel Castelli, V, Greco, ML & Graniti, A 2002, 'A new syringopeptin produced by bean strains of Pseudomonas syringae pv. syringae', Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology, vol. 1597, no. 1, pp. 81-89. https://doi.org/10.1016/S0167-4838(02)00283-2

A new syringopeptin produced by bean strains of Pseudomonas syringae pv. syringae. / Grgurina, Ingeborg; Mariotti, Feliciana; Fogliano, Vincenzo; Gallo, Monica; Scaloni, Andrea; Iacobellis, Nicola S.; Lo Cantore, Pietro; Mannina, Luisa; Van Axel Castelli, Valeria; Greco, Maria Luigia; Graniti, Antonio.

In: Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology, Vol. 1597, No. 1, 20.05.2002, p. 81-89.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - A new syringopeptin produced by bean strains of Pseudomonas syringae pv. syringae

AU - Grgurina, Ingeborg

AU - Mariotti, Feliciana

AU - Fogliano, Vincenzo

AU - Gallo, Monica

AU - Scaloni, Andrea

AU - Iacobellis, Nicola S.

AU - Lo Cantore, Pietro

AU - Mannina, Luisa

AU - Van Axel Castelli, Valeria

AU - Greco, Maria Luigia

AU - Graniti, Antonio

PY - 2002/5/20

Y1 - 2002/5/20

N2 - Two strains (B728a and Y37) of the phytopathogenic bacterium Pseudomonas syringae pv. syringae isolated from bean (Phaseolus vulgaris) plants were shown to produce in culture both syringomycin, a lipodepsinonapeptide secreted by the majority of the strains of the bacterium, and a new form of syringopeptin, SP22Phv. The structure of the latter metabolite was elucidated by the combined use of mass spectrometry (MS), nuclear magnetic resonance (NMR) spectroscopy and chemical procedures. Comparative phytotoxic and antimicrobial assays showed that SP22Phv did not differ substantially from the previously characterized syringopeptin 22 (SP22) as far as toxicity to plants was concerned, but was less active in inhibiting the growth of the test fungi Rhodotorula pilimanae and Geotrichum candidum and of the Gram-positive bacterium Bacillus megaterium.

AB - Two strains (B728a and Y37) of the phytopathogenic bacterium Pseudomonas syringae pv. syringae isolated from bean (Phaseolus vulgaris) plants were shown to produce in culture both syringomycin, a lipodepsinonapeptide secreted by the majority of the strains of the bacterium, and a new form of syringopeptin, SP22Phv. The structure of the latter metabolite was elucidated by the combined use of mass spectrometry (MS), nuclear magnetic resonance (NMR) spectroscopy and chemical procedures. Comparative phytotoxic and antimicrobial assays showed that SP22Phv did not differ substantially from the previously characterized syringopeptin 22 (SP22) as far as toxicity to plants was concerned, but was less active in inhibiting the growth of the test fungi Rhodotorula pilimanae and Geotrichum candidum and of the Gram-positive bacterium Bacillus megaterium.

KW - Lipodepsipeptide

KW - Phytotoxin

KW - Pseudomonas syringae pv. syringae

KW - Structure

KW - Syringopeptin 22 Phv

U2 - 10.1016/S0167-4838(02)00283-2

DO - 10.1016/S0167-4838(02)00283-2

M3 - Article

C2 - 12009406

AN - SCOPUS:18344381198

VL - 1597

SP - 81

EP - 89

JO - Biochimica et biophysica acta-protein structure and molecular enzymology

JF - Biochimica et biophysica acta-protein structure and molecular enzymology

SN - 0167-4838

IS - 1

ER -