A New High-Throughput Tool to Screen Mosquito-Borne Viruses in Zika Virus Endemic/Epidemic Areas

Sara Moutailler; Lena Yousfi; Laurence Mousson; Elodie Devillers; Marie Vazeille; Anubis Vega-Rúa; Yvon Perrin; Frédéric Jourdain; Fabrice Chandre; Arnaud Cannet; Sandrine Chantilly; Johana Restrepo; Amandine Guidez; Isabelle Dusfour; Filipe Vieira Santos de Abreu; Taissa Pereira Dos Santos; Davy Jiolle; Tessa M. Visser; Constantianus J.M. Koenraadt; Merril Wongsokarijo; Mawlouth Diallo; Diawo Diallo; Alioune Gaye; Sébastien Boyer; Veasna Duong; Géraldine Piorkowski; Christophe Paupy; Ricardo Lourenco de Oliveira; Xavier de Lamballerie; Anna Bella Failloux

doi:10.3390/v11100904

A New High-Throughput Tool to Screen Mosquito-Borne Viruses in Zika Virus Endemic/Epidemic Areas

Sara Moutailler^*, Lena Yousfi, Laurence Mousson, Elodie Devillers, Marie Vazeille, Anubis Vega-Rúa, Yvon Perrin, Frédéric Jourdain, Fabrice Chandre, Arnaud Cannet, Sandrine Chantilly, Johana Restrepo, Amandine Guidez, Isabelle Dusfour, Filipe Vieira Santos de Abreu, Taissa Pereira Dos Santos, Davy Jiolle, Tessa M. Visser, Constantianus J.M. Koenraadt, Merril WongsokarijoMawlouth Diallo, Diawo Diallo, Alioune Gaye, Sébastien Boyer, Veasna Duong, Géraldine Piorkowski, Christophe Paupy, Ricardo Lourenco de Oliveira, Xavier de Lamballerie, Anna Bella Failloux

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

11 Citations (Scopus)

Abstract

Mosquitoes are vectors of arboviruses affecting animal and human health. Arboviruses circulate primarily within an enzootic cycle and recurrent spillovers contribute to the emergence of human-adapted viruses able to initiate an urban cycle involving anthropophilic mosquitoes. The increasing volume of travel and trade offers multiple opportunities for arbovirus introduction in new regions. This scenario has been exemplified recently with the Zika pandemic. To incriminate a mosquito as vector of a pathogen, several criteria are required such as the detection of natural infections in mosquitoes. In this study, we used a high-throughput chip based on the BioMark™ Dynamic arrays system capable of detecting 64 arboviruses in a single experiment. A total of 17,958 mosquitoes collected in Zika-endemic/epidemic countries (Brazil, French Guiana, Guadeloupe, Suriname, Senegal, and Cambodia) were analyzed. Here we show that this new tool can detect endemic and epidemic viruses in different mosquito species in an epidemic context. Thus, this fast and low-cost method can be suggested as a novel epidemiological surveillance tool to identify circulating arboviruses.

Original language	English
Article number	904
Journal	Viruses
Volume	11
Issue number	10
DOIs	https://doi.org/10.3390/v11100904
Publication status	Published - 27 Sep 2019

Keywords

microfluidic analysis
molecular epidemiology
mosquito-borne viruses
surveillance

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3390/v11100904Licence: CC BY

https://edepot.wur.nl/503203Licence: CC BY

Zika virus polyprotein gene, complete cds
Moutailler, S. (Creator), Yousfi, L. (Creator), Mousson, L. (Creator), Devillers, E. (Creator), Vazeille, M. (Creator), Vega-Rúa, A. (Creator), Perrin, Y. (Creator), Jourdain, F. (Creator), Chandre, F. (Creator), Cannet, A. (Creator), Chantilly, S. (Creator), Restrepo, J. (Creator), Guidez, A. (Creator), Dusfour, I. (Creator), Vieira Santos de Abreu, F. (Creator), Pereira Dos Santos, T. (Creator), Jiolle, D. (Creator), Visser, T. (Creator), Koenraadt, S. (Creator), Wongsokarijo, M. (Creator), Diallo, M. (Creator), Diallo, D. (Creator), Gaye, A. (Creator), Boyer, S. (Creator), Duong, V. (Creator), Piorkowski, G. (Creator), Paupy, C. (Creator), Lourenco de Oliveira, R. (Creator), de Lamballerie, X. (Creator) & Failloux, A. B. (Creator), 17 Oct 2019
https://www.ncbi.nlm.nih.gov/popset/1707337925
Dataset

ZIKAlliance: A global alliance for Zika virus control and prevention
1/10/16 → 30/09/21
Project: EU research project

Cite this

Moutailler, S., Yousfi, L., Mousson, L., Devillers, E., Vazeille, M., Vega-Rúa, A., Perrin, Y., Jourdain, F., Chandre, F., Cannet, A., Chantilly, S., Restrepo, J., Guidez, A., Dusfour, I., Vieira Santos de Abreu, F., Pereira Dos Santos, T., Jiolle, D., Visser, T. M., Koenraadt, C. J. M., ... Failloux, A. B. (2019). A New High-Throughput Tool to Screen Mosquito-Borne Viruses in Zika Virus Endemic/Epidemic Areas. Viruses, 11(10), [904]. https://doi.org/10.3390/v11100904

@article{a3ef59ec512c42ac88ce3d328008b416,

title = "A New High-Throughput Tool to Screen Mosquito-Borne Viruses in Zika Virus Endemic/Epidemic Areas",

abstract = "Mosquitoes are vectors of arboviruses affecting animal and human health. Arboviruses circulate primarily within an enzootic cycle and recurrent spillovers contribute to the emergence of human-adapted viruses able to initiate an urban cycle involving anthropophilic mosquitoes. The increasing volume of travel and trade offers multiple opportunities for arbovirus introduction in new regions. This scenario has been exemplified recently with the Zika pandemic. To incriminate a mosquito as vector of a pathogen, several criteria are required such as the detection of natural infections in mosquitoes. In this study, we used a high-throughput chip based on the BioMark{\texttrademark} Dynamic arrays system capable of detecting 64 arboviruses in a single experiment. A total of 17,958 mosquitoes collected in Zika-endemic/epidemic countries (Brazil, French Guiana, Guadeloupe, Suriname, Senegal, and Cambodia) were analyzed. Here we show that this new tool can detect endemic and epidemic viruses in different mosquito species in an epidemic context. Thus, this fast and low-cost method can be suggested as a novel epidemiological surveillance tool to identify circulating arboviruses.",

keywords = "microfluidic analysis, molecular epidemiology, mosquito-borne viruses, surveillance",

author = "Sara Moutailler and Lena Yousfi and Laurence Mousson and Elodie Devillers and Marie Vazeille and Anubis Vega-R{\'u}a and Yvon Perrin and Fr{\'e}d{\'e}ric Jourdain and Fabrice Chandre and Arnaud Cannet and Sandrine Chantilly and Johana Restrepo and Amandine Guidez and Isabelle Dusfour and {Vieira Santos de Abreu}, Filipe and {Pereira Dos Santos}, Taissa and Davy Jiolle and Visser, {Tessa M.} and Koenraadt, {Constantianus J.M.} and Merril Wongsokarijo and Mawlouth Diallo and Diawo Diallo and Alioune Gaye and S{\'e}bastien Boyer and Veasna Duong and G{\'e}raldine Piorkowski and Christophe Paupy and {Lourenco de Oliveira}, Ricardo and {de Lamballerie}, Xavier and Failloux, {Anna Bella}",

year = "2019",

month = sep,

day = "27",

doi = "10.3390/v11100904",

language = "English",

volume = "11",

journal = "Viruses",

issn = "1999-4915",

publisher = "MDPI",

number = "10",

}

Moutailler, S, Yousfi, L, Mousson, L, Devillers, E, Vazeille, M, Vega-Rúa, A, Perrin, Y, Jourdain, F, Chandre, F, Cannet, A, Chantilly, S, Restrepo, J, Guidez, A, Dusfour, I, Vieira Santos de Abreu, F, Pereira Dos Santos, T, Jiolle, D, Visser, TM , Koenraadt, CJM, Wongsokarijo, M, Diallo, M, Diallo, D, Gaye, A, Boyer, S, Duong, V, Piorkowski, G, Paupy, C, Lourenco de Oliveira, R, de Lamballerie, X & Failloux, AB 2019, 'A New High-Throughput Tool to Screen Mosquito-Borne Viruses in Zika Virus Endemic/Epidemic Areas', Viruses, vol. 11, no. 10, 904. https://doi.org/10.3390/v11100904

TY - JOUR

T1 - A New High-Throughput Tool to Screen Mosquito-Borne Viruses in Zika Virus Endemic/Epidemic Areas

AU - Moutailler, Sara

AU - Yousfi, Lena

AU - Mousson, Laurence

AU - Devillers, Elodie

AU - Vazeille, Marie

AU - Vega-Rúa, Anubis

AU - Perrin, Yvon

AU - Jourdain, Frédéric

AU - Chandre, Fabrice

AU - Cannet, Arnaud

AU - Chantilly, Sandrine

AU - Restrepo, Johana

AU - Guidez, Amandine

AU - Dusfour, Isabelle

AU - Vieira Santos de Abreu, Filipe

AU - Pereira Dos Santos, Taissa

AU - Jiolle, Davy

AU - Visser, Tessa M.

AU - Koenraadt, Constantianus J.M.

AU - Wongsokarijo, Merril

AU - Diallo, Mawlouth

AU - Diallo, Diawo

AU - Gaye, Alioune

AU - Boyer, Sébastien

AU - Duong, Veasna

AU - Piorkowski, Géraldine

AU - Paupy, Christophe

AU - Lourenco de Oliveira, Ricardo

AU - de Lamballerie, Xavier

AU - Failloux, Anna Bella

PY - 2019/9/27

Y1 - 2019/9/27

N2 - Mosquitoes are vectors of arboviruses affecting animal and human health. Arboviruses circulate primarily within an enzootic cycle and recurrent spillovers contribute to the emergence of human-adapted viruses able to initiate an urban cycle involving anthropophilic mosquitoes. The increasing volume of travel and trade offers multiple opportunities for arbovirus introduction in new regions. This scenario has been exemplified recently with the Zika pandemic. To incriminate a mosquito as vector of a pathogen, several criteria are required such as the detection of natural infections in mosquitoes. In this study, we used a high-throughput chip based on the BioMark™ Dynamic arrays system capable of detecting 64 arboviruses in a single experiment. A total of 17,958 mosquitoes collected in Zika-endemic/epidemic countries (Brazil, French Guiana, Guadeloupe, Suriname, Senegal, and Cambodia) were analyzed. Here we show that this new tool can detect endemic and epidemic viruses in different mosquito species in an epidemic context. Thus, this fast and low-cost method can be suggested as a novel epidemiological surveillance tool to identify circulating arboviruses.

AB - Mosquitoes are vectors of arboviruses affecting animal and human health. Arboviruses circulate primarily within an enzootic cycle and recurrent spillovers contribute to the emergence of human-adapted viruses able to initiate an urban cycle involving anthropophilic mosquitoes. The increasing volume of travel and trade offers multiple opportunities for arbovirus introduction in new regions. This scenario has been exemplified recently with the Zika pandemic. To incriminate a mosquito as vector of a pathogen, several criteria are required such as the detection of natural infections in mosquitoes. In this study, we used a high-throughput chip based on the BioMark™ Dynamic arrays system capable of detecting 64 arboviruses in a single experiment. A total of 17,958 mosquitoes collected in Zika-endemic/epidemic countries (Brazil, French Guiana, Guadeloupe, Suriname, Senegal, and Cambodia) were analyzed. Here we show that this new tool can detect endemic and epidemic viruses in different mosquito species in an epidemic context. Thus, this fast and low-cost method can be suggested as a novel epidemiological surveillance tool to identify circulating arboviruses.

KW - microfluidic analysis

KW - molecular epidemiology

KW - mosquito-borne viruses

KW - surveillance

U2 - 10.3390/v11100904

DO - 10.3390/v11100904

M3 - Article

C2 - 31569736

AN - SCOPUS:85072769653

VL - 11

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 10

M1 - 904

ER -

A New High-Throughput Tool to Screen Mosquito-Borne Viruses in Zika Virus Endemic/Epidemic Areas

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Datasets

Zika virus polyprotein gene, complete cds

Projects

ZIKAlliance: A global alliance for Zika virus control and prevention

Cite this