A new formula to calculate activity of superoxide dismutase in indirect assays

Chen Zhang, Marieke E. Bruins, Zhi Qiang Yang, Shu Tao Liu*, Ping Fan Rao

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

To calculate superoxide dismutase (SOD) activity rapidly and accurately by indirect SOD assays, a formula based on the ratio of the catalytic speed of SOD to the reaction speed of the indicator with superoxide anion was deduced. The accuracy of this formula was compared with the conventional formula based on inhibition in five indirect SOD assays. The new formula was validated in nearly the entire SOD activity range, whereas the conventional formula was validated only during inhibition of 40-60%. This formula might also be used for the assays of other enzymes.

Original languageEnglish
Pages (from-to)65-67
JournalAnalytical Biochemistry
Volume503
DOIs
Publication statusPublished - 2016

Keywords

  • Cytochrome c
  • Epinephrine
  • Hydroxylamine hydrochloride
  • Nitroblue tetrazolium
  • SOD
  • WST

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