A new formula to calculate activity of superoxide dismutase in indirect assays

Chen Zhang; Marieke E. Bruins; Zhi Qiang Yang; Shu Tao Liu; Ping Fan Rao

doi:10.1016/j.ab.2016.03.014

A new formula to calculate activity of superoxide dismutase in indirect assays

Chen Zhang, Marieke E. Bruins, Zhi Qiang Yang, Shu Tao Liu^*, Ping Fan Rao

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

23 Citations (Scopus)

Abstract

To calculate superoxide dismutase (SOD) activity rapidly and accurately by indirect SOD assays, a formula based on the ratio of the catalytic speed of SOD to the reaction speed of the indicator with superoxide anion was deduced. The accuracy of this formula was compared with the conventional formula based on inhibition in five indirect SOD assays. The new formula was validated in nearly the entire SOD activity range, whereas the conventional formula was validated only during inhibition of 40-60%. This formula might also be used for the assays of other enzymes.

Original language	English
Pages (from-to)	65-67
Journal	Analytical Biochemistry
Volume	503
DOIs	https://doi.org/10.1016/j.ab.2016.03.014
Publication status	Published - 2016

Keywords

Cytochrome c
Epinephrine
Hydroxylamine hydrochloride
Nitroblue tetrazolium
SOD
WST

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10.1016/j.ab.2016.03.014

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title = "A new formula to calculate activity of superoxide dismutase in indirect assays",

abstract = "To calculate superoxide dismutase (SOD) activity rapidly and accurately by indirect SOD assays, a formula based on the ratio of the catalytic speed of SOD to the reaction speed of the indicator with superoxide anion was deduced. The accuracy of this formula was compared with the conventional formula based on inhibition in five indirect SOD assays. The new formula was validated in nearly the entire SOD activity range, whereas the conventional formula was validated only during inhibition of 40-60%. This formula might also be used for the assays of other enzymes.",

keywords = "Cytochrome c, Epinephrine, Hydroxylamine hydrochloride, Nitroblue tetrazolium, SOD, WST",

author = "Chen Zhang and Bruins, {Marieke E.} and Yang, {Zhi Qiang} and Liu, {Shu Tao} and Rao, {Ping Fan}",

year = "2016",

doi = "10.1016/j.ab.2016.03.014",

language = "English",

volume = "503",

pages = "65--67",

journal = "Analytical Biochemistry",

issn = "0003-2697",

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TY - JOUR

T1 - A new formula to calculate activity of superoxide dismutase in indirect assays

AU - Zhang, Chen

AU - Bruins, Marieke E.

AU - Yang, Zhi Qiang

AU - Liu, Shu Tao

AU - Rao, Ping Fan

PY - 2016

Y1 - 2016

N2 - To calculate superoxide dismutase (SOD) activity rapidly and accurately by indirect SOD assays, a formula based on the ratio of the catalytic speed of SOD to the reaction speed of the indicator with superoxide anion was deduced. The accuracy of this formula was compared with the conventional formula based on inhibition in five indirect SOD assays. The new formula was validated in nearly the entire SOD activity range, whereas the conventional formula was validated only during inhibition of 40-60%. This formula might also be used for the assays of other enzymes.

AB - To calculate superoxide dismutase (SOD) activity rapidly and accurately by indirect SOD assays, a formula based on the ratio of the catalytic speed of SOD to the reaction speed of the indicator with superoxide anion was deduced. The accuracy of this formula was compared with the conventional formula based on inhibition in five indirect SOD assays. The new formula was validated in nearly the entire SOD activity range, whereas the conventional formula was validated only during inhibition of 40-60%. This formula might also be used for the assays of other enzymes.

KW - Cytochrome c

KW - Epinephrine

KW - Hydroxylamine hydrochloride

KW - Nitroblue tetrazolium

KW - SOD

KW - WST

U2 - 10.1016/j.ab.2016.03.014

DO - 10.1016/j.ab.2016.03.014

M3 - Article

AN - SCOPUS:84964584135

VL - 503

SP - 65

EP - 67

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

ER -

A new formula to calculate activity of superoxide dismutase in indirect assays

Abstract

Keywords

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