A new extraction procedure to abate the burden of non-extractable antibiotic residues in manure

Larissa J.M. Jansen*, Milou G.M. van de Schans, Diana de Boer, Irma E.A. Bongers, Heike Schmitt, Paul Hoeksma, Bjorn J.A. Berendsen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Through agricultural soil fertilization using organic manure, antibiotic residues can accumulate in the environment. In order to assess the risks of environmental pollution by veterinary drugs, monitoring of manure for antibiotic residues is necessary. As manure is a complex matrix, extraction of antibiotics proved to be challenging. In this study, 24 extraction solvents were assessed for the extraction of residues from manure representing ten antibiotics from the antibiotic classes tetracyclines, quinolones, macrolides, lincosamides and sulfonamides. Especially for the tetracyclines and quinolones the extraction solvent selection is critical, due to high fractions of non-extractable residues especially when using aqueous solvents (62–77% and 90–95% respectively when using milli-Q water). In contrast, sulfonamides can effectively be extracted with aqueous solvents. Overall, 0.125% trifluoroacetic acid in acetonitrile in combination with McIlvain-EDTA buffer proved to be the most effective extraction solvent. A longitudinal study pointed out that most antibiotics bind to solid manure particles instantaneously after addition. Trimethoprim is an exception, but because by using the optimal extraction solvent, the optimum fraction of bound residues is desorbed, this does not hamper quantitative analysis when using spiked manure quality control samples. Based on these new insights, the current in-house multi-residue LC-MS/MS method for manure analysis, containing 48 antibiotics, was revised, additionally validated and applied to 34 incurred manure samples.

Original languageEnglish
Pages (from-to)544-553
Number of pages10
JournalChemosphere
Volume224
DOIs
Publication statusPublished - 1 Jun 2019

Fingerprint

Manure
Manures
Antibiotics
antibiotics
manure
Anti-Bacterial Agents
Solvent extraction
Tetracyclines
Quinolones
Sulfonamides
Lincosamides
Veterinary Drugs
Trifluoroacetic acid
Trifluoroacetic Acid
Environmental Pollution
Trimethoprim
Drug Monitoring
Ethylenediaminetetraacetic acid
Macrolides
Acetonitrile

Keywords

  • Antibiotics
  • Extraction
  • LC-MS
  • Manure
  • Non-extractable residues

Cite this

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title = "A new extraction procedure to abate the burden of non-extractable antibiotic residues in manure",
abstract = "Through agricultural soil fertilization using organic manure, antibiotic residues can accumulate in the environment. In order to assess the risks of environmental pollution by veterinary drugs, monitoring of manure for antibiotic residues is necessary. As manure is a complex matrix, extraction of antibiotics proved to be challenging. In this study, 24 extraction solvents were assessed for the extraction of residues from manure representing ten antibiotics from the antibiotic classes tetracyclines, quinolones, macrolides, lincosamides and sulfonamides. Especially for the tetracyclines and quinolones the extraction solvent selection is critical, due to high fractions of non-extractable residues especially when using aqueous solvents (62–77{\%} and 90–95{\%} respectively when using milli-Q water). In contrast, sulfonamides can effectively be extracted with aqueous solvents. Overall, 0.125{\%} trifluoroacetic acid in acetonitrile in combination with McIlvain-EDTA buffer proved to be the most effective extraction solvent. A longitudinal study pointed out that most antibiotics bind to solid manure particles instantaneously after addition. Trimethoprim is an exception, but because by using the optimal extraction solvent, the optimum fraction of bound residues is desorbed, this does not hamper quantitative analysis when using spiked manure quality control samples. Based on these new insights, the current in-house multi-residue LC-MS/MS method for manure analysis, containing 48 antibiotics, was revised, additionally validated and applied to 34 incurred manure samples.",
keywords = "Antibiotics, Extraction, LC-MS, Manure, Non-extractable residues",
author = "Jansen, {Larissa J.M.} and {van de Schans}, {Milou G.M.} and {de Boer}, Diana and Bongers, {Irma E.A.} and Heike Schmitt and Paul Hoeksma and Berendsen, {Bjorn J.A.}",
year = "2019",
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language = "English",
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A new extraction procedure to abate the burden of non-extractable antibiotic residues in manure. / Jansen, Larissa J.M.; van de Schans, Milou G.M.; de Boer, Diana; Bongers, Irma E.A.; Schmitt, Heike; Hoeksma, Paul; Berendsen, Bjorn J.A.

In: Chemosphere, Vol. 224, 01.06.2019, p. 544-553.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - A new extraction procedure to abate the burden of non-extractable antibiotic residues in manure

AU - Jansen, Larissa J.M.

AU - van de Schans, Milou G.M.

AU - de Boer, Diana

AU - Bongers, Irma E.A.

AU - Schmitt, Heike

AU - Hoeksma, Paul

AU - Berendsen, Bjorn J.A.

PY - 2019/6/1

Y1 - 2019/6/1

N2 - Through agricultural soil fertilization using organic manure, antibiotic residues can accumulate in the environment. In order to assess the risks of environmental pollution by veterinary drugs, monitoring of manure for antibiotic residues is necessary. As manure is a complex matrix, extraction of antibiotics proved to be challenging. In this study, 24 extraction solvents were assessed for the extraction of residues from manure representing ten antibiotics from the antibiotic classes tetracyclines, quinolones, macrolides, lincosamides and sulfonamides. Especially for the tetracyclines and quinolones the extraction solvent selection is critical, due to high fractions of non-extractable residues especially when using aqueous solvents (62–77% and 90–95% respectively when using milli-Q water). In contrast, sulfonamides can effectively be extracted with aqueous solvents. Overall, 0.125% trifluoroacetic acid in acetonitrile in combination with McIlvain-EDTA buffer proved to be the most effective extraction solvent. A longitudinal study pointed out that most antibiotics bind to solid manure particles instantaneously after addition. Trimethoprim is an exception, but because by using the optimal extraction solvent, the optimum fraction of bound residues is desorbed, this does not hamper quantitative analysis when using spiked manure quality control samples. Based on these new insights, the current in-house multi-residue LC-MS/MS method for manure analysis, containing 48 antibiotics, was revised, additionally validated and applied to 34 incurred manure samples.

AB - Through agricultural soil fertilization using organic manure, antibiotic residues can accumulate in the environment. In order to assess the risks of environmental pollution by veterinary drugs, monitoring of manure for antibiotic residues is necessary. As manure is a complex matrix, extraction of antibiotics proved to be challenging. In this study, 24 extraction solvents were assessed for the extraction of residues from manure representing ten antibiotics from the antibiotic classes tetracyclines, quinolones, macrolides, lincosamides and sulfonamides. Especially for the tetracyclines and quinolones the extraction solvent selection is critical, due to high fractions of non-extractable residues especially when using aqueous solvents (62–77% and 90–95% respectively when using milli-Q water). In contrast, sulfonamides can effectively be extracted with aqueous solvents. Overall, 0.125% trifluoroacetic acid in acetonitrile in combination with McIlvain-EDTA buffer proved to be the most effective extraction solvent. A longitudinal study pointed out that most antibiotics bind to solid manure particles instantaneously after addition. Trimethoprim is an exception, but because by using the optimal extraction solvent, the optimum fraction of bound residues is desorbed, this does not hamper quantitative analysis when using spiked manure quality control samples. Based on these new insights, the current in-house multi-residue LC-MS/MS method for manure analysis, containing 48 antibiotics, was revised, additionally validated and applied to 34 incurred manure samples.

KW - Antibiotics

KW - Extraction

KW - LC-MS

KW - Manure

KW - Non-extractable residues

U2 - 10.1016/j.chemosphere.2019.02.166

DO - 10.1016/j.chemosphere.2019.02.166

M3 - Article

VL - 224

SP - 544

EP - 553

JO - Chemosphere

JF - Chemosphere

SN - 0045-6535

ER -