A new and versatile method for the successful conversion of AFLP-TM markers into simple single locus markers

B.W. Brugmans, R.G.M. van der Hulst, R.G.F. Visser, W.H. Lindhout, H. van Eck

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Genetic markers can efficiently be obtained by using amplified fragment length polymorphism (AFLP) fingerprinting because no prior information on DNA sequence is required. However, the conversion of AFLP markers from complex fingerprints into simple single locus assays is perceived as problematic because DNA sequence information is required for the design of new locus-specific PCR primers. In addition, single locus polymorphism (SNP) information is required to design an allele-specific assay. This paper describes a new and versatile method for the conversion of AFLP markers into simple assays. The protocol presented in this paper offers solutions for frequently occurring pitfalls and describes a procedure for the identification of the SNP responsible for the AFLP. By following this approach, a high success rate for the conversion of AFLP markers into locus-specific markers was obtained.
Original languageEnglish
Pages (from-to)e55
Number of pages10
JournalNucleic acids research
Volume31
Issue number10
DOIs
Publication statusPublished - 2003

Keywords

  • fragment-length-polymorphism
  • pcr-based markers
  • suppressed recombination
  • f-2 populations
  • rflp markers
  • dna
  • resistance
  • barley
  • gene
  • map

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