A naturally occurring nucleotide polymorphism in the orf2/folc promoter is associated with Streptococcus suis virulence

A. de Greeff; H. Buys; J.M. Wells; H.E. Smith

doi:10.1186/s12866-014-0264-9

A naturally occurring nucleotide polymorphism in the orf2/folc promoter is associated with Streptococcus suis virulence

A. de Greeff, H. Buys, J.M. Wells, H.E. Smith

Research output: Contribution to journal › Article › Academic › peer-review

3 Citations (Scopus)

Abstract

Streptococcus suis is a major problem in the swine industry causing meningitis, arthritis and pericarditis in piglets. Pathogenesis of S. suis is poorly understood. We previously showed that introduction of a 3 kb genomic fragment from virulent serotype 2 strain 10 into a weakly virulent serotype 2 strain S735, generated a hypervirulent isolate. The 3 kb genomic fragment contained two complete open reading frames (ORF) in an operon-structure of which one ORF showed similarity to folylpolyglutamate synthetase, whereas the function of the second ORF could not be predicted based on database searches for protein similarity.

Original language	English
Article number	264
Journal	BMC Microbiology
Volume	14
Issue number	1
DOIs	https://doi.org/10.1186/s12866-014-0264-9
Publication status	Published - 2014

Keywords

germ-free pigs
serotype-2
strains
identification
cytokine
pathogen
suilysin
release
type-2
cells

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title = "A naturally occurring nucleotide polymorphism in the orf2/folc promoter is associated with Streptococcus suis virulence",

abstract = "Streptococcus suis is a major problem in the swine industry causing meningitis, arthritis and pericarditis in piglets. Pathogenesis of S. suis is poorly understood. We previously showed that introduction of a 3 kb genomic fragment from virulent serotype 2 strain 10 into a weakly virulent serotype 2 strain S735, generated a hypervirulent isolate. The 3 kb genomic fragment contained two complete open reading frames (ORF) in an operon-structure of which one ORF showed similarity to folylpolyglutamate synthetase, whereas the function of the second ORF could not be predicted based on database searches for protein similarity.",

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AU - de Greeff, A.

AU - Buys, H.

AU - Wells, J.M.

AU - Smith, H.E.

PY - 2014

Y1 - 2014

N2 - Streptococcus suis is a major problem in the swine industry causing meningitis, arthritis and pericarditis in piglets. Pathogenesis of S. suis is poorly understood. We previously showed that introduction of a 3 kb genomic fragment from virulent serotype 2 strain 10 into a weakly virulent serotype 2 strain S735, generated a hypervirulent isolate. The 3 kb genomic fragment contained two complete open reading frames (ORF) in an operon-structure of which one ORF showed similarity to folylpolyglutamate synthetase, whereas the function of the second ORF could not be predicted based on database searches for protein similarity.

AB - Streptococcus suis is a major problem in the swine industry causing meningitis, arthritis and pericarditis in piglets. Pathogenesis of S. suis is poorly understood. We previously showed that introduction of a 3 kb genomic fragment from virulent serotype 2 strain 10 into a weakly virulent serotype 2 strain S735, generated a hypervirulent isolate. The 3 kb genomic fragment contained two complete open reading frames (ORF) in an operon-structure of which one ORF showed similarity to folylpolyglutamate synthetase, whereas the function of the second ORF could not be predicted based on database searches for protein similarity.

KW - germ-free pigs

KW - serotype-2

KW - strains

KW - identification

KW - cytokine

KW - pathogen

KW - suilysin

KW - release

KW - type-2

KW - cells

U2 - 10.1186/s12866-014-0264-9

DO - 10.1186/s12866-014-0264-9

M3 - Article

VL - 14

JO - BMC Microbiology

JF - BMC Microbiology

SN - 1471-2180

IS - 1

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ER -