A naturally occurring nucleotide polymorphism in the orf2/folc promoter is associated with Streptococcus suis virulence

A. de Greeff, H. Buys, J.M. Wells, H.E. Smith

Research output: Contribution to journal › Article › Academic › peer-review

3 Citations (Scopus)

Abstract

Streptococcus suis is a major problem in the swine industry causing meningitis, arthritis and pericarditis in piglets. Pathogenesis of S. suis is poorly understood. We previously showed that introduction of a 3 kb genomic fragment from virulent serotype 2 strain 10 into a weakly virulent serotype 2 strain S735, generated a hypervirulent isolate. The 3 kb genomic fragment contained two complete open reading frames (ORF) in an operon-structure of which one ORF showed similarity to folylpolyglutamate synthetase, whereas the function of the second ORF could not be predicted based on database searches for protein similarity.
Original languageEnglish
Article number264
JournalBMC Microbiology
Volume14
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

Streptococcus suis
Open Reading Frames
Virulence
Nucleotides
Protein Databases
Pericarditis
Operon
Meningitis
Arthritis
Industry
Swine
Serogroup

Keywords

  • germ-free pigs
  • serotype-2
  • strains
  • identification
  • cytokine
  • pathogen
  • suilysin
  • release
  • type-2
  • cells

Cite this

de Greeff, A. ; Buys, H. ; Wells, J.M. ; Smith, H.E. / A naturally occurring nucleotide polymorphism in the orf2/folc promoter is associated with Streptococcus suis virulence. In: BMC Microbiology. 2014 ; Vol. 14, No. 1.
@article{a8c5c921b89948baa7cc4f4282e5d2c0,
title = "A naturally occurring nucleotide polymorphism in the orf2/folc promoter is associated with Streptococcus suis virulence",
abstract = "Streptococcus suis is a major problem in the swine industry causing meningitis, arthritis and pericarditis in piglets. Pathogenesis of S. suis is poorly understood. We previously showed that introduction of a 3 kb genomic fragment from virulent serotype 2 strain 10 into a weakly virulent serotype 2 strain S735, generated a hypervirulent isolate. The 3 kb genomic fragment contained two complete open reading frames (ORF) in an operon-structure of which one ORF showed similarity to folylpolyglutamate synthetase, whereas the function of the second ORF could not be predicted based on database searches for protein similarity.",
keywords = "germ-free pigs, serotype-2, strains, identification, cytokine, pathogen, suilysin, release, type-2, cells",
author = "{de Greeff}, A. and H. Buys and J.M. Wells and H.E. Smith",
year = "2014",
doi = "10.1186/s12866-014-0264-9",
language = "English",
volume = "14",
journal = "BMC Microbiology",
issn = "1471-2180",
publisher = "Springer Verlag",
number = "1",

}

de Greeff, A, Buys, H, Wells, JM & Smith, HE 2014, 'A naturally occurring nucleotide polymorphism in the orf2/folc promoter is associated with Streptococcus suis virulence', BMC Microbiology, vol. 14, no. 1, 264. https://doi.org/10.1186/s12866-014-0264-9

A naturally occurring nucleotide polymorphism in the orf2/folc promoter is associated with Streptococcus suis virulence. / de Greeff, A.; Buys, H.; Wells, J.M.; Smith, H.E.

In: BMC Microbiology, Vol. 14, No. 1, 264, 2014.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - A naturally occurring nucleotide polymorphism in the orf2/folc promoter is associated with Streptococcus suis virulence

AU - de Greeff, A.

AU - Buys, H.

AU - Wells, J.M.

AU - Smith, H.E.

PY - 2014

Y1 - 2014

N2 - Streptococcus suis is a major problem in the swine industry causing meningitis, arthritis and pericarditis in piglets. Pathogenesis of S. suis is poorly understood. We previously showed that introduction of a 3 kb genomic fragment from virulent serotype 2 strain 10 into a weakly virulent serotype 2 strain S735, generated a hypervirulent isolate. The 3 kb genomic fragment contained two complete open reading frames (ORF) in an operon-structure of which one ORF showed similarity to folylpolyglutamate synthetase, whereas the function of the second ORF could not be predicted based on database searches for protein similarity.

AB - Streptococcus suis is a major problem in the swine industry causing meningitis, arthritis and pericarditis in piglets. Pathogenesis of S. suis is poorly understood. We previously showed that introduction of a 3 kb genomic fragment from virulent serotype 2 strain 10 into a weakly virulent serotype 2 strain S735, generated a hypervirulent isolate. The 3 kb genomic fragment contained two complete open reading frames (ORF) in an operon-structure of which one ORF showed similarity to folylpolyglutamate synthetase, whereas the function of the second ORF could not be predicted based on database searches for protein similarity.

KW - germ-free pigs

KW - serotype-2

KW - strains

KW - identification

KW - cytokine

KW - pathogen

KW - suilysin

KW - release

KW - type-2

KW - cells

U2 - 10.1186/s12866-014-0264-9

DO - 10.1186/s12866-014-0264-9

M3 - Article

VL - 14

JO - BMC Microbiology

JF - BMC Microbiology

SN - 1471-2180

IS - 1

M1 - 264

ER -

de Greeff A, Buys H, Wells JM, Smith HE. A naturally occurring nucleotide polymorphism in the orf2/folc promoter is associated with Streptococcus suis virulence. BMC Microbiology. 2014;14(1). 264. https://doi.org/10.1186/s12866-014-0264-9

Access to Document