A Molecular Toolbox to Identify and Quantify Grape Varieties: On the Trace of “Glera”

Ilaria Carrara, Valeria Terzi*, Roberta Ghizzoni, Stefano Delbono, Giorgio Tumino, Manna Crespan, Massimo Gardiman, Enrico Francia, Caterina Morcia

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)


A pillar of wine authenticity is the variety/ies used. Ampelographic descriptors and SSR markers, included in several national and international databases, are extensively used for varietal identification purposes. Recently, SNP markers have been proposed as useful for grape varietal identification and traceability. Our study has been directed toward the development of a molecular toolbox able to track grape varieties from the nursery to the must. Two complementary approaches were developed, exploiting SNP markers with two different technologies, i.e., a high-throughput platform for varietal identification and a digital PCR system for varietal quantification. As proof-of-concept, the toolbox was successfully applied to the identification and quantification of the “Glera” variety along the Prosecco wine production chain. The assays developed found their limits in commercial, aged wines.

Original languageEnglish
Article number3091
Issue number16
Publication statusPublished - Aug 2023


  • chip digital PCR
  • grape chain authenticity
  • high-throughput SNP genotyping
  • varietal traceability


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