A molecular diagnostic for tropical race 4 of the banana fusarium wilt pathogen

M.A. Dita Rodriguez, C. Waalwijk, I.W. Buddenhagen, M.T. Souza, G.H.J. Kema

Research output: Contribution to journalArticleAcademicpeer-review

114 Citations (Scopus)

Abstract

This study analysed genomic variation of the translation elongation factor 1a (TEF-1a) and the intergenic spacer region (IGS) of the nuclear ribosomal operon of Fusarium oxysporum f. sp. cubense (Foc) isolates, from different banana production areas, representing strains within the known races, comprising 20 vegetative compatibility groups (VCG). Based on two single nucleotide polymorphisms present in the IGS region, a PCR-based diagnostic tool was developed to specifically detect isolates from VCG 01213, also called tropical race 4 (TR4), which is currently a major concern in global banana production. Validation involved TR4 isolates, as well as Foc isolates from 19 other VCGs, other fungal plant pathogens and DNA samples from infected tissues of the Cavendish banana cultivar Grand Naine (AAA). Subsequently, a multiplex PCR was developed for fungal or plant samples that also discriminated Musa acuminata and M. balbisiana genotypes. It was concluded that this diagnostic procedure is currently the best option for the rapid and reliable detection and monitoring of TR4 to support eradication and quarantine strategies
Original languageEnglish
Pages (from-to)348-357
JournalPlant Pathology
Volume59
Issue number2
DOIs
Publication statusPublished - 2010

Keywords

  • f-sp cubense
  • vegetative compatibility groups
  • genetic-variation
  • panama-disease
  • oxysporum
  • pcr
  • quantification
  • identification
  • australia
  • tissue

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