A modified extraction protocol enables detection and quantification of celiac disease-related gluten proteins from wheat

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Abstract

The detection, analysis, and quantification of individual celiac disease (CD) immune responsive gluten proteins in wheat and related cereals (barley, rye) require an adequate and reliable extraction protocol. Because different types of gluten proteins behave differently in terms of solubility, currently different extraction protocols exist. The performance of various documented gluten extraction protocols is evaluated for specificity and completeness by gel electrophoresis (SDS-PAGE), immunoblotting and RIDASCREEN® Gliadin competitive ELISA. Based on these results, an optimized, two-step extraction protocol has been developed
Original languageEnglish
Pages (from-to)975-982
JournalJournal of Chromatography. B, Analytical technologies in the biomedical and life sciences
Volume877
Issue number10
DOIs
Publication statusPublished - 2009

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Keywords

  • high-molecular-weight
  • monoclonal-antibody
  • gel-electrophoresis
  • endosperm proteins
  • gliadin
  • subunits
  • foods
  • performance
  • peptides
  • epitopes

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