A mechanistic model to study the effect of the cell wall on starch digestion in intact cotyledon cells

Ana M. Rovalino-Córdova*, Victor Aguirre Montesdeoca, Edoardo Capuano

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The role of the plant matrix is recognized as the main factor restricting starch digestibility in beans. Several authors have provided insights about the mechanisms behind the reduced starch digestibility in plant matrices. In this study, by means of a mathematical model, we provide a mechanistic explanation of the role played by the cell wall. It was confirmed that starch entrapped within intact cells could only be hydrolysed after α-amylase diffusion through the cell wall. This process is limited by the pores naturally present in the cell wall and the adsorption of α-amylase to the cell wall surface. These factors restrict the concentration of α-amylase available within the cells. The model assumptions are valid under controlled laboratory conditions and were validated with in-vitro digestion data giving very accurate results. The proposed approach provides new information to understand the digestibility of starch, and possibly other macronutrients, in complex food matrices.

Original languageEnglish
Article number117351
JournalCarbohydrate Polymers
Volume253
Early online dateNov 2020
DOIs
Publication statusPublished - 1 Feb 2021

Keywords

  • Adsorption
  • Cell wall
  • Food matrix
  • Mechanistic model
  • Starch digestion
  • α-amylase

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