A Lowly Digestible-Starch Diet after Weaning Enhances Exogenous Glucose Oxidation Rate in Female, but Not in Male, Mice

José M.S. Fernández-Calleja, Lianne M.S. Bouwman, Hans J.M. Swarts, Nils Billecke, Annemarie Oosting, Jaap Keijer, Evert M. van Schothorst*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Starches of low digestibility are associated with improved glucose metabolism. We hypothesise that a lowly digestible-starch diet (LDD) versus a highly digestible-starch diet (HDD) improves the capacity to oxidise starch, and that this is sex-dependent. Mice were fed a LDD or a HDD for 3 weeks directly after weaning. Body weight (BW), body composition (BC), and digestible energy intake (dEI) were determined weekly. At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry. Pancreatic amylase activity and total 13C hepatic enrichment were determined in females immediately before and 4 h after administration of the starch bolus. For both sexes, BW, BC, and basal EE and RER were not affected by the type of starch, but dEI and hydrogen production were increased by the LDD. Only in females, total carbohydrate oxidation and starch-derived glucose oxidation in response to the starch bolus were higher in LDD versus HDD mice; this was not accompanied by differences in amylase activity or hepatic partitioning of the 13C label. These results show that starch digestibility impacts glucose metabolism differently in females versus males.

Original languageEnglish
Article number2242
JournalNutrients
Volume11
Issue number9
DOIs
Publication statusPublished - 18 Sep 2019

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Weaning
Starch
weaning
starch
oxidation
Diet
Glucose
glucose
mice
diet
hydrogen production
digestible energy
Amylases
Body Composition
Energy Intake
amylases
energy expenditure
Energy Metabolism
body composition
Hydrogen

Keywords

  • 13C-starch
  • amylase
  • amylopectin
  • amylose
  • C57BL mice
  • glucose oxidation
  • glycaemic index
  • indirect calorimetry

Cite this

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title = "A Lowly Digestible-Starch Diet after Weaning Enhances Exogenous Glucose Oxidation Rate in Female, but Not in Male, Mice",
abstract = "Starches of low digestibility are associated with improved glucose metabolism. We hypothesise that a lowly digestible-starch diet (LDD) versus a highly digestible-starch diet (HDD) improves the capacity to oxidise starch, and that this is sex-dependent. Mice were fed a LDD or a HDD for 3 weeks directly after weaning. Body weight (BW), body composition (BC), and digestible energy intake (dEI) were determined weekly. At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry. Pancreatic amylase activity and total 13C hepatic enrichment were determined in females immediately before and 4 h after administration of the starch bolus. For both sexes, BW, BC, and basal EE and RER were not affected by the type of starch, but dEI and hydrogen production were increased by the LDD. Only in females, total carbohydrate oxidation and starch-derived glucose oxidation in response to the starch bolus were higher in LDD versus HDD mice; this was not accompanied by differences in amylase activity or hepatic partitioning of the 13C label. These results show that starch digestibility impacts glucose metabolism differently in females versus males.",
keywords = "13C-starch, amylase, amylopectin, amylose, C57BL mice, glucose oxidation, glycaemic index, indirect calorimetry",
author = "Fern{\'a}ndez-Calleja, {Jos{\'e} M.S.} and Bouwman, {Lianne M.S.} and Swarts, {Hans J.M.} and Nils Billecke and Annemarie Oosting and Jaap Keijer and {van Schothorst}, {Evert M.}",
year = "2019",
month = "9",
day = "18",
doi = "10.3390/nu11092242",
language = "English",
volume = "11",
journal = "Nutrients",
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A Lowly Digestible-Starch Diet after Weaning Enhances Exogenous Glucose Oxidation Rate in Female, but Not in Male, Mice. / Fernández-Calleja, José M.S.; Bouwman, Lianne M.S.; Swarts, Hans J.M.; Billecke, Nils; Oosting, Annemarie; Keijer, Jaap; van Schothorst, Evert M.

In: Nutrients, Vol. 11, No. 9, 2242, 18.09.2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - A Lowly Digestible-Starch Diet after Weaning Enhances Exogenous Glucose Oxidation Rate in Female, but Not in Male, Mice

AU - Fernández-Calleja, José M.S.

AU - Bouwman, Lianne M.S.

AU - Swarts, Hans J.M.

AU - Billecke, Nils

AU - Oosting, Annemarie

AU - Keijer, Jaap

AU - van Schothorst, Evert M.

PY - 2019/9/18

Y1 - 2019/9/18

N2 - Starches of low digestibility are associated with improved glucose metabolism. We hypothesise that a lowly digestible-starch diet (LDD) versus a highly digestible-starch diet (HDD) improves the capacity to oxidise starch, and that this is sex-dependent. Mice were fed a LDD or a HDD for 3 weeks directly after weaning. Body weight (BW), body composition (BC), and digestible energy intake (dEI) were determined weekly. At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry. Pancreatic amylase activity and total 13C hepatic enrichment were determined in females immediately before and 4 h after administration of the starch bolus. For both sexes, BW, BC, and basal EE and RER were not affected by the type of starch, but dEI and hydrogen production were increased by the LDD. Only in females, total carbohydrate oxidation and starch-derived glucose oxidation in response to the starch bolus were higher in LDD versus HDD mice; this was not accompanied by differences in amylase activity or hepatic partitioning of the 13C label. These results show that starch digestibility impacts glucose metabolism differently in females versus males.

AB - Starches of low digestibility are associated with improved glucose metabolism. We hypothesise that a lowly digestible-starch diet (LDD) versus a highly digestible-starch diet (HDD) improves the capacity to oxidise starch, and that this is sex-dependent. Mice were fed a LDD or a HDD for 3 weeks directly after weaning. Body weight (BW), body composition (BC), and digestible energy intake (dEI) were determined weekly. At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry. Pancreatic amylase activity and total 13C hepatic enrichment were determined in females immediately before and 4 h after administration of the starch bolus. For both sexes, BW, BC, and basal EE and RER were not affected by the type of starch, but dEI and hydrogen production were increased by the LDD. Only in females, total carbohydrate oxidation and starch-derived glucose oxidation in response to the starch bolus were higher in LDD versus HDD mice; this was not accompanied by differences in amylase activity or hepatic partitioning of the 13C label. These results show that starch digestibility impacts glucose metabolism differently in females versus males.

KW - 13C-starch

KW - amylase

KW - amylopectin

KW - amylose

KW - C57BL mice

KW - glucose oxidation

KW - glycaemic index

KW - indirect calorimetry

U2 - 10.3390/nu11092242

DO - 10.3390/nu11092242

M3 - Article

VL - 11

JO - Nutrients

JF - Nutrients

SN - 2072-6643

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M1 - 2242

ER -