A lipidomic analysis approach to evaluate the response to cholesterol-lowering food intake

E. Szymanska, F.A. van Dorsten, J. Troost, I. Paliukhovich, E.J.J. van Velzen, M.M.W.B. Hendriks, E.A. Trautwein, J.P.M. van Duynhoven, R.J. Vreeken, A.K. Smilde

Research output: Contribution to journalArticleAcademicpeer-review

28 Citations (Scopus)

Abstract

Plant sterols (PS) are well known to reduce serum levels of total cholesterol and LDL-cholesterol. Lipidomics potentially provides detailed information on a wide range of individual serum lipid metabolites, which may further add to our understanding of the biological effects of PS. In this study, lipidomics analysis was applied to serum samples from a placebo-controlled, parallel human intervention study (n = 97) of 4-week consumption of two PS-enriched, yoghurt drinks differing in fat content (based on 0.1% vs. 1.5% dairy fat). A comprehensive data analysis strategy was developed and implemented to assess and compare effects of two different PS-treatments and placebo treatment. The combination of univariate and multivariate data analysis approaches allowed to show significant effects of PS intake on the serum lipidome, and helped to distinguish them from fat content and non-specific effects. The PS-enriched 0.1% dairy fat yoghurt drink had a stronger impact on the lipidome than the 1.5% dairy fat yoghurt drink, despite similar LDL-cholesterol lowering effects. The PS-enriched 0.1% dairy fat yoghurt drink reduced levels of several sphingomyelins which correlated well with the reduction in LDL-cholesterol and can be explained by co-localization of sphingomyelins and cholesterol on the surface of LDL lipoprotein. Statistically significant reductions in serum levels of two lysophosphatidylcholines (LPC(16:1), LPC(20:1)) and cholesteryl arachidonate may suggest reduced inflammation and atherogenic potential. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-011-0384-2) contains supplementary material, which is available to authorized users.
Original languageEnglish
Pages (from-to)894-906
JournalMetabolomics
Volume8
Issue number5
DOIs
Publication statusPublished - 2012

Fingerprint

Phytosterols
Eating
Cholesterol
Yogurt
Dairies
Fats
LDL Cholesterol
Serum
Sphingomyelins
Placebos
Lysophosphatidylcholines
Metabolites
LDL Lipoproteins
Multivariate Analysis
Inflammation
Lipids
Therapeutics

Keywords

  • coronary-artery-disease
  • plant sterols
  • mass-spectrometry
  • risk-factor
  • plasma
  • sphingomyelin
  • profiles
  • intervention
  • inflammation
  • metabolism

Cite this

Szymanska, E., van Dorsten, F. A., Troost, J., Paliukhovich, I., van Velzen, E. J. J., Hendriks, M. M. W. B., ... Smilde, A. K. (2012). A lipidomic analysis approach to evaluate the response to cholesterol-lowering food intake. Metabolomics, 8(5), 894-906. https://doi.org/10.1007/s11306-011-0384-2
Szymanska, E. ; van Dorsten, F.A. ; Troost, J. ; Paliukhovich, I. ; van Velzen, E.J.J. ; Hendriks, M.M.W.B. ; Trautwein, E.A. ; van Duynhoven, J.P.M. ; Vreeken, R.J. ; Smilde, A.K. / A lipidomic analysis approach to evaluate the response to cholesterol-lowering food intake. In: Metabolomics. 2012 ; Vol. 8, No. 5. pp. 894-906.
@article{af65fe5ebee743fea639d91433094a55,
title = "A lipidomic analysis approach to evaluate the response to cholesterol-lowering food intake",
abstract = "Plant sterols (PS) are well known to reduce serum levels of total cholesterol and LDL-cholesterol. Lipidomics potentially provides detailed information on a wide range of individual serum lipid metabolites, which may further add to our understanding of the biological effects of PS. In this study, lipidomics analysis was applied to serum samples from a placebo-controlled, parallel human intervention study (n = 97) of 4-week consumption of two PS-enriched, yoghurt drinks differing in fat content (based on 0.1{\%} vs. 1.5{\%} dairy fat). A comprehensive data analysis strategy was developed and implemented to assess and compare effects of two different PS-treatments and placebo treatment. The combination of univariate and multivariate data analysis approaches allowed to show significant effects of PS intake on the serum lipidome, and helped to distinguish them from fat content and non-specific effects. The PS-enriched 0.1{\%} dairy fat yoghurt drink had a stronger impact on the lipidome than the 1.5{\%} dairy fat yoghurt drink, despite similar LDL-cholesterol lowering effects. The PS-enriched 0.1{\%} dairy fat yoghurt drink reduced levels of several sphingomyelins which correlated well with the reduction in LDL-cholesterol and can be explained by co-localization of sphingomyelins and cholesterol on the surface of LDL lipoprotein. Statistically significant reductions in serum levels of two lysophosphatidylcholines (LPC(16:1), LPC(20:1)) and cholesteryl arachidonate may suggest reduced inflammation and atherogenic potential. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-011-0384-2) contains supplementary material, which is available to authorized users.",
keywords = "coronary-artery-disease, plant sterols, mass-spectrometry, risk-factor, plasma, sphingomyelin, profiles, intervention, inflammation, metabolism",
author = "E. Szymanska and {van Dorsten}, F.A. and J. Troost and I. Paliukhovich and {van Velzen}, E.J.J. and M.M.W.B. Hendriks and E.A. Trautwein and {van Duynhoven}, J.P.M. and R.J. Vreeken and A.K. Smilde",
year = "2012",
doi = "10.1007/s11306-011-0384-2",
language = "English",
volume = "8",
pages = "894--906",
journal = "Metabolomics",
issn = "1573-3882",
publisher = "Springer New York",
number = "5",

}

Szymanska, E, van Dorsten, FA, Troost, J, Paliukhovich, I, van Velzen, EJJ, Hendriks, MMWB, Trautwein, EA, van Duynhoven, JPM, Vreeken, RJ & Smilde, AK 2012, 'A lipidomic analysis approach to evaluate the response to cholesterol-lowering food intake', Metabolomics, vol. 8, no. 5, pp. 894-906. https://doi.org/10.1007/s11306-011-0384-2

A lipidomic analysis approach to evaluate the response to cholesterol-lowering food intake. / Szymanska, E.; van Dorsten, F.A.; Troost, J.; Paliukhovich, I.; van Velzen, E.J.J.; Hendriks, M.M.W.B.; Trautwein, E.A.; van Duynhoven, J.P.M.; Vreeken, R.J.; Smilde, A.K.

In: Metabolomics, Vol. 8, No. 5, 2012, p. 894-906.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - A lipidomic analysis approach to evaluate the response to cholesterol-lowering food intake

AU - Szymanska, E.

AU - van Dorsten, F.A.

AU - Troost, J.

AU - Paliukhovich, I.

AU - van Velzen, E.J.J.

AU - Hendriks, M.M.W.B.

AU - Trautwein, E.A.

AU - van Duynhoven, J.P.M.

AU - Vreeken, R.J.

AU - Smilde, A.K.

PY - 2012

Y1 - 2012

N2 - Plant sterols (PS) are well known to reduce serum levels of total cholesterol and LDL-cholesterol. Lipidomics potentially provides detailed information on a wide range of individual serum lipid metabolites, which may further add to our understanding of the biological effects of PS. In this study, lipidomics analysis was applied to serum samples from a placebo-controlled, parallel human intervention study (n = 97) of 4-week consumption of two PS-enriched, yoghurt drinks differing in fat content (based on 0.1% vs. 1.5% dairy fat). A comprehensive data analysis strategy was developed and implemented to assess and compare effects of two different PS-treatments and placebo treatment. The combination of univariate and multivariate data analysis approaches allowed to show significant effects of PS intake on the serum lipidome, and helped to distinguish them from fat content and non-specific effects. The PS-enriched 0.1% dairy fat yoghurt drink had a stronger impact on the lipidome than the 1.5% dairy fat yoghurt drink, despite similar LDL-cholesterol lowering effects. The PS-enriched 0.1% dairy fat yoghurt drink reduced levels of several sphingomyelins which correlated well with the reduction in LDL-cholesterol and can be explained by co-localization of sphingomyelins and cholesterol on the surface of LDL lipoprotein. Statistically significant reductions in serum levels of two lysophosphatidylcholines (LPC(16:1), LPC(20:1)) and cholesteryl arachidonate may suggest reduced inflammation and atherogenic potential. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-011-0384-2) contains supplementary material, which is available to authorized users.

AB - Plant sterols (PS) are well known to reduce serum levels of total cholesterol and LDL-cholesterol. Lipidomics potentially provides detailed information on a wide range of individual serum lipid metabolites, which may further add to our understanding of the biological effects of PS. In this study, lipidomics analysis was applied to serum samples from a placebo-controlled, parallel human intervention study (n = 97) of 4-week consumption of two PS-enriched, yoghurt drinks differing in fat content (based on 0.1% vs. 1.5% dairy fat). A comprehensive data analysis strategy was developed and implemented to assess and compare effects of two different PS-treatments and placebo treatment. The combination of univariate and multivariate data analysis approaches allowed to show significant effects of PS intake on the serum lipidome, and helped to distinguish them from fat content and non-specific effects. The PS-enriched 0.1% dairy fat yoghurt drink had a stronger impact on the lipidome than the 1.5% dairy fat yoghurt drink, despite similar LDL-cholesterol lowering effects. The PS-enriched 0.1% dairy fat yoghurt drink reduced levels of several sphingomyelins which correlated well with the reduction in LDL-cholesterol and can be explained by co-localization of sphingomyelins and cholesterol on the surface of LDL lipoprotein. Statistically significant reductions in serum levels of two lysophosphatidylcholines (LPC(16:1), LPC(20:1)) and cholesteryl arachidonate may suggest reduced inflammation and atherogenic potential. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-011-0384-2) contains supplementary material, which is available to authorized users.

KW - coronary-artery-disease

KW - plant sterols

KW - mass-spectrometry

KW - risk-factor

KW - plasma

KW - sphingomyelin

KW - profiles

KW - intervention

KW - inflammation

KW - metabolism

U2 - 10.1007/s11306-011-0384-2

DO - 10.1007/s11306-011-0384-2

M3 - Article

VL - 8

SP - 894

EP - 906

JO - Metabolomics

JF - Metabolomics

SN - 1573-3882

IS - 5

ER -

Szymanska E, van Dorsten FA, Troost J, Paliukhovich I, van Velzen EJJ, Hendriks MMWB et al. A lipidomic analysis approach to evaluate the response to cholesterol-lowering food intake. Metabolomics. 2012;8(5):894-906. https://doi.org/10.1007/s11306-011-0384-2