A High-Throughput Approach for Photosynthesis Studies in a Brassicaceae Panel

Sofia A. Bengoa Luoni*, Francesco Garassino, Mark G.M. Aarts

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

The study of natural variations in photosynthesis in the Brassicaceae family offers the possibility of identifying mechanisms to enhance photosynthetic efficiency in crop plants. Indeed, this family, and particularly its tribe Brassiceae, has been shown to harbor species that have a higher-than-expected photosynthetic efficiency, possibly as a result of a complex evolutionary history. Over the past two decades, methods have been developed to measure photosynthetic efficiency based on chlorophyll fluorescence. Chlorophyll fluorescence measurements are performed with special cameras, such as the FluorCams, which can be included in robotic systems to create high-throughput phenotyping platforms. While these platforms have so far demonstrated high efficiency in measuring small model species like Arabidopsis thaliana, they have the drawback of limited adaptability to accommodate different plant sizes. As a result, the range of species that can be analyzed is restricted. This chapter presents our approach to analyze the photosynthetic parameters: ϕPSII and Fv/Fm for a panel of Brassicaceae species, including a high-photosynthesis species, Hirschfeldia incana, and the adaptations to the phenotyping platform that are required to accommodate this varied group of plants.

Original languageEnglish
Title of host publicationPlant Functional Genomics
Subtitle of host publicationMethods and Protocols, Volume 1
EditorsF. Maghuly
Place of PublicationNew York
PublisherHumana Press
Pages39-53
Number of pages15
ISBN (Electronic)9781071637784
ISBN (Print)9781071637777, 9781071637807
DOIs
Publication statusPublished - 25 Apr 2024

Publication series

NameMethods in Molecular Biology
Volume2787
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Brassicaceae
  • Chlorophyll fluorescence
  • FluorCam
  • High-throughput phenotyping
  • Hirschfeldia incana
  • Photosynthesis
  • Photosystem II

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