A high-resolution map of the H1 locus harbouring resistance to the potato cyst nematode Globodera rostochiensis

E.H. Bakker; U. Achenbach; J.D. Bakker; J.M. van Vliet; J. Peleman; B. Segers; S. van der Heijden; P. van der Linde; R. Graveland; R.C.B. Hutten; H.J. van Eck; E.R. Coppoolse; E.A.G. van der Vossen; A. Goverse

doi:10.1007/s00122-004-1606-z

A high-resolution map of the H1 locus harbouring resistance to the potato cyst nematode Globodera rostochiensis

E.H. Bakker, U. Achenbach, J.D. Bakker, J.M. van Vliet, J. Peleman, B. Segers, S. van der Heijden, P. van der Linde, R. Graveland, R.C.B. Hutten, H.J. van Eck, E.R. Coppoolse, E.A.G. van der Vossen, A. Goverse

Research output: Contribution to journal › Article › Academic › peer-review

52 Citations (Scopus)

Abstract

The resistance gene H1 confers resistance to the potato cyst nematode Globodera rostochiensis and is located at the distal end of the long arm of chromosome V of potato. For marker enrichment of the H1 locus, a bulked segregant analysis (BSA) was carried out using 704 AFLP primer combinations. A second source of markers tightly linked to H1 is the ultra-high-density (UHD) genetic map of the potato cross SH x RH. This map has been produced with 387 AFLP primer combinations and consists of 10,365 AFLP markers in 1,118 bins (http://www.dpw.wageningen-ur.nl/uhd/). Comparing these two methods revealed that BSA resulted in one marker/cM and the UHD map in four markers/cM in the H1 interval. Subsequently, a high-resolution genetic map of the H1 locus has been developed using a segregating F-1 SH x RH population consisting of 1,209 genotypes. Two PCR-based markers were designed at either side of the H1 gene to screen the 1,209 genotypes for recombination events. In the high-resolution genetic map, two of the four co-segregating AFLP markers could be separated from the H1 gene. Marker EM1 is located at a distance of 0.2 cM, and marker EM14 is located at a distance of 0.8 cM. The other two co-segregating markers CM1 (in coupling) and EM15 (in repulsion) could not be separated from the H1 gene.

Original language	English
Pages (from-to)	146-152
Journal	Theoretical and Applied Genetics
Volume	109
Issue number	1
DOIs	https://doi.org/10.1007/s00122-004-1606-z
Publication status	Published - 2004

Keywords

gene conferring resistance
broad-spectrum resistance
disease resistance
markers
tomato
virus
cluster
identification
strategy
andigena

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Bakker, E. H., Achenbach, U., Bakker, J. D., van Vliet, J. M., Peleman, J., Segers, B., van der Heijden, S., van der Linde, P., Graveland, R., Hutten, R. C. B., van Eck, H. J., Coppoolse, E. R., van der Vossen, E. A. G., & Goverse, A. (2004). A high-resolution map of the H1 locus harbouring resistance to the potato cyst nematode Globodera rostochiensis. Theoretical and Applied Genetics, 109(1), 146-152. https://doi.org/10.1007/s00122-004-1606-z

Bakker, E.H. ; Achenbach, U. ; Bakker, J.D. ; van Vliet, J.M. ; Peleman, J. ; Segers, B. ; van der Heijden, S. ; van der Linde, P. ; Graveland, R. ; Hutten, R.C.B. ; van Eck, H.J. ; Coppoolse, E.R. ; van der Vossen, E.A.G. ; Goverse, A. / A high-resolution map of the H1 locus harbouring resistance to the potato cyst nematode Globodera rostochiensis. In: Theoretical and Applied Genetics. 2004 ; Vol. 109, No. 1. pp. 146-152.

@article{220a347bc8d140c090c13c68721ea777,

title = "A high-resolution map of the H1 locus harbouring resistance to the potato cyst nematode Globodera rostochiensis",

abstract = "The resistance gene H1 confers resistance to the potato cyst nematode Globodera rostochiensis and is located at the distal end of the long arm of chromosome V of potato. For marker enrichment of the H1 locus, a bulked segregant analysis (BSA) was carried out using 704 AFLP primer combinations. A second source of markers tightly linked to H1 is the ultra-high-density (UHD) genetic map of the potato cross SH x RH. This map has been produced with 387 AFLP primer combinations and consists of 10,365 AFLP markers in 1,118 bins (http://www.dpw.wageningen-ur.nl/uhd/). Comparing these two methods revealed that BSA resulted in one marker/cM and the UHD map in four markers/cM in the H1 interval. Subsequently, a high-resolution genetic map of the H1 locus has been developed using a segregating F-1 SH x RH population consisting of 1,209 genotypes. Two PCR-based markers were designed at either side of the H1 gene to screen the 1,209 genotypes for recombination events. In the high-resolution genetic map, two of the four co-segregating AFLP markers could be separated from the H1 gene. Marker EM1 is located at a distance of 0.2 cM, and marker EM14 is located at a distance of 0.8 cM. The other two co-segregating markers CM1 (in coupling) and EM15 (in repulsion) could not be separated from the H1 gene.",

keywords = "gene conferring resistance, broad-spectrum resistance, disease resistance, markers, tomato, virus, cluster, identification, strategy, andigena",

author = "E.H. Bakker and U. Achenbach and J.D. Bakker and {van Vliet}, J.M. and J. Peleman and B. Segers and {van der Heijden}, S. and {van der Linde}, P. and R. Graveland and R.C.B. Hutten and {van Eck}, H.J. and E.R. Coppoolse and {van der Vossen}, E.A.G. and A. Goverse",

year = "2004",

doi = "10.1007/s00122-004-1606-z",

language = "English",

volume = "109",

pages = "146--152",

journal = "Theoretical and Applied Genetics",

issn = "0040-5752",

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Bakker, EH, Achenbach, U, Bakker, JD, van Vliet, JM, Peleman, J, Segers, B, van der Heijden, S, van der Linde, P, Graveland, R, Hutten, RCB , van Eck, HJ, Coppoolse, ER, van der Vossen, EAG & Goverse, A 2004, 'A high-resolution map of the H1 locus harbouring resistance to the potato cyst nematode Globodera rostochiensis', Theoretical and Applied Genetics, vol. 109, no. 1, pp. 146-152. https://doi.org/10.1007/s00122-004-1606-z

A high-resolution map of the H1 locus harbouring resistance to the potato cyst nematode Globodera rostochiensis. / Bakker, E.H.; Achenbach, U.; Bakker, J.D.; van Vliet, J.M.; Peleman, J.; Segers, B.; van der Heijden, S.; van der Linde, P.; Graveland, R.; Hutten, R.C.B.; van Eck, H.J.; Coppoolse, E.R.; van der Vossen, E.A.G.; Goverse, A.

In: Theoretical and Applied Genetics, Vol. 109, No. 1, 2004, p. 146-152.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - A high-resolution map of the H1 locus harbouring resistance to the potato cyst nematode Globodera rostochiensis

AU - Bakker, E.H.

AU - Achenbach, U.

AU - Bakker, J.D.

AU - van Vliet, J.M.

AU - Peleman, J.

AU - Segers, B.

AU - van der Heijden, S.

AU - van der Linde, P.

AU - Graveland, R.

AU - Hutten, R.C.B.

AU - van Eck, H.J.

AU - Coppoolse, E.R.

AU - van der Vossen, E.A.G.

AU - Goverse, A.

PY - 2004

Y1 - 2004

N2 - The resistance gene H1 confers resistance to the potato cyst nematode Globodera rostochiensis and is located at the distal end of the long arm of chromosome V of potato. For marker enrichment of the H1 locus, a bulked segregant analysis (BSA) was carried out using 704 AFLP primer combinations. A second source of markers tightly linked to H1 is the ultra-high-density (UHD) genetic map of the potato cross SH x RH. This map has been produced with 387 AFLP primer combinations and consists of 10,365 AFLP markers in 1,118 bins (http://www.dpw.wageningen-ur.nl/uhd/). Comparing these two methods revealed that BSA resulted in one marker/cM and the UHD map in four markers/cM in the H1 interval. Subsequently, a high-resolution genetic map of the H1 locus has been developed using a segregating F-1 SH x RH population consisting of 1,209 genotypes. Two PCR-based markers were designed at either side of the H1 gene to screen the 1,209 genotypes for recombination events. In the high-resolution genetic map, two of the four co-segregating AFLP markers could be separated from the H1 gene. Marker EM1 is located at a distance of 0.2 cM, and marker EM14 is located at a distance of 0.8 cM. The other two co-segregating markers CM1 (in coupling) and EM15 (in repulsion) could not be separated from the H1 gene.

AB - The resistance gene H1 confers resistance to the potato cyst nematode Globodera rostochiensis and is located at the distal end of the long arm of chromosome V of potato. For marker enrichment of the H1 locus, a bulked segregant analysis (BSA) was carried out using 704 AFLP primer combinations. A second source of markers tightly linked to H1 is the ultra-high-density (UHD) genetic map of the potato cross SH x RH. This map has been produced with 387 AFLP primer combinations and consists of 10,365 AFLP markers in 1,118 bins (http://www.dpw.wageningen-ur.nl/uhd/). Comparing these two methods revealed that BSA resulted in one marker/cM and the UHD map in four markers/cM in the H1 interval. Subsequently, a high-resolution genetic map of the H1 locus has been developed using a segregating F-1 SH x RH population consisting of 1,209 genotypes. Two PCR-based markers were designed at either side of the H1 gene to screen the 1,209 genotypes for recombination events. In the high-resolution genetic map, two of the four co-segregating AFLP markers could be separated from the H1 gene. Marker EM1 is located at a distance of 0.2 cM, and marker EM14 is located at a distance of 0.8 cM. The other two co-segregating markers CM1 (in coupling) and EM15 (in repulsion) could not be separated from the H1 gene.

KW - gene conferring resistance

KW - broad-spectrum resistance

KW - disease resistance

KW - markers

KW - tomato

KW - virus

KW - cluster

KW - identification

KW - strategy

KW - andigena

U2 - 10.1007/s00122-004-1606-z

DO - 10.1007/s00122-004-1606-z

M3 - Article

VL - 109

SP - 146

EP - 152

JO - Theoretical and Applied Genetics

JF - Theoretical and Applied Genetics

SN - 0040-5752

IS - 1

ER -