A high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells

R.D. Hall; T. Riksen-Bruinsma; G. Weyens; I.J. Rosquin; R.N. Denys; I.J. Evans; J.E. Lathouwers; M.P. LefObvre; J.M. Dunwell; A. van Tunen; F.A. Krens

doi:10.1038/nbt0996-1133

A high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells

R.D. Hall, T. Riksen-Bruinsma, G. Weyens, I.J. Rosquin, R.N. Denys, I.J. Evans, J.E. Lathouwers, M.P. LefObvre, J.M. Dunwell, A. van Tunen, F.A. Krens

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Abstract

An optimized protocol has been developed for the efficient and rapid genetic modification of sugar beet (Beta vulgaris L). A polyethylene glycol- mediated DNA transformation technique could be applied to protoplast populations enriched specifically for a single totipotent cell type derived from stomatal guard cells, to achieve high transformation frequencies. Bialaphos resistance, conferred by the pat gene, produced a highly efficient selection system. The majority of plants were obtained within 8 to 9 weeks and were appropriate for plant breeding purposes. All were resistant to glufosinate-ammonium-based herbicides. Detailed genomic characterization has verified transgene integration, and progeny analysis showed Mendelian inheritance.

Original language	English
Pages (from-to)	1133-1138
Journal	Nature Biotechnology
Volume	14
Issue number	9
DOIs	https://doi.org/10.1038/nbt0996-1133
Publication status	Published - 1996

Keywords

Beta vulgaris
Herbicide resistance
Transformation

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title = "A high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells",

abstract = "An optimized protocol has been developed for the efficient and rapid genetic modification of sugar beet (Beta vulgaris L). A polyethylene glycol- mediated DNA transformation technique could be applied to protoplast populations enriched specifically for a single totipotent cell type derived from stomatal guard cells, to achieve high transformation frequencies. Bialaphos resistance, conferred by the pat gene, produced a highly efficient selection system. The majority of plants were obtained within 8 to 9 weeks and were appropriate for plant breeding purposes. All were resistant to glufosinate-ammonium-based herbicides. Detailed genomic characterization has verified transgene integration, and progeny analysis showed Mendelian inheritance.",

keywords = "Beta vulgaris, Herbicide resistance, Transformation",

author = "R.D. Hall and T. Riksen-Bruinsma and G. Weyens and I.J. Rosquin and R.N. Denys and I.J. Evans and J.E. Lathouwers and M.P. LefObvre and J.M. Dunwell and {van Tunen}, A. and F.A. Krens",

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doi = "10.1038/nbt0996-1133",

language = "English",

volume = "14",

pages = "1133--1138",

journal = "Nature Biotechnology",

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TY - JOUR

T1 - A high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells

AU - Hall, R.D.

AU - Riksen-Bruinsma, T.

AU - Weyens, G.

AU - Rosquin, I.J.

AU - Denys, R.N.

AU - Evans, I.J.

AU - Lathouwers, J.E.

AU - LefObvre, M.P.

AU - Dunwell, J.M.

AU - van Tunen, A.

AU - Krens, F.A.

PY - 1996

Y1 - 1996

N2 - An optimized protocol has been developed for the efficient and rapid genetic modification of sugar beet (Beta vulgaris L). A polyethylene glycol- mediated DNA transformation technique could be applied to protoplast populations enriched specifically for a single totipotent cell type derived from stomatal guard cells, to achieve high transformation frequencies. Bialaphos resistance, conferred by the pat gene, produced a highly efficient selection system. The majority of plants were obtained within 8 to 9 weeks and were appropriate for plant breeding purposes. All were resistant to glufosinate-ammonium-based herbicides. Detailed genomic characterization has verified transgene integration, and progeny analysis showed Mendelian inheritance.

AB - An optimized protocol has been developed for the efficient and rapid genetic modification of sugar beet (Beta vulgaris L). A polyethylene glycol- mediated DNA transformation technique could be applied to protoplast populations enriched specifically for a single totipotent cell type derived from stomatal guard cells, to achieve high transformation frequencies. Bialaphos resistance, conferred by the pat gene, produced a highly efficient selection system. The majority of plants were obtained within 8 to 9 weeks and were appropriate for plant breeding purposes. All were resistant to glufosinate-ammonium-based herbicides. Detailed genomic characterization has verified transgene integration, and progeny analysis showed Mendelian inheritance.

KW - Beta vulgaris

KW - Herbicide resistance

KW - Transformation

U2 - 10.1038/nbt0996-1133

DO - 10.1038/nbt0996-1133

M3 - Article

SN - 1087-0156

VL - 14

SP - 1133

EP - 1138

JO - Nature Biotechnology

JF - Nature Biotechnology

IS - 9

ER -

A high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells

Abstract

Keywords

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