A high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells

R.D. Hall, T. Riksen-Bruinsma, G. Weyens, I.J. Rosquin, R.N. Denys, I.J. Evans, J.E. Lathouwers, M.P. LefObvre, J.M. Dunwell, A. van Tunen, F.A. Krens

    Research output: Contribution to journalArticleAcademicpeer-review

    76 Citations (Scopus)

    Abstract

    An optimized protocol has been developed for the efficient and rapid genetic modification of sugar beet (Beta vulgaris L). A polyethylene glycol- mediated DNA transformation technique could be applied to protoplast populations enriched specifically for a single totipotent cell type derived from stomatal guard cells, to achieve high transformation frequencies. Bialaphos resistance, conferred by the pat gene, produced a highly efficient selection system. The majority of plants were obtained within 8 to 9 weeks and were appropriate for plant breeding purposes. All were resistant to glufosinate-ammonium-based herbicides. Detailed genomic characterization has verified transgene integration, and progeny analysis showed Mendelian inheritance.
    Original languageEnglish
    Pages (from-to)1133-1138
    JournalNature Biotechnology
    Volume14
    Issue number9
    DOIs
    Publication statusPublished - 1996

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