A high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells

R.D. Hall, T. Riksen-Bruinsma, G. Weyens, I.J. Rosquin, R.N. Denys, I.J. Evans, J.E. Lathouwers, M.P. LefObvre, J.M. Dunwell, A. van Tunen, F.A. Krens

    Research output: Contribution to journalArticleAcademicpeer-review

    76 Citations (Scopus)

    Abstract

    An optimized protocol has been developed for the efficient and rapid genetic modification of sugar beet (Beta vulgaris L). A polyethylene glycol- mediated DNA transformation technique could be applied to protoplast populations enriched specifically for a single totipotent cell type derived from stomatal guard cells, to achieve high transformation frequencies. Bialaphos resistance, conferred by the pat gene, produced a highly efficient selection system. The majority of plants were obtained within 8 to 9 weeks and were appropriate for plant breeding purposes. All were resistant to glufosinate-ammonium-based herbicides. Detailed genomic characterization has verified transgene integration, and progeny analysis showed Mendelian inheritance.
    Original languageEnglish
    Pages (from-to)1133-1138
    JournalNature Biotechnology
    Volume14
    Issue number9
    DOIs
    Publication statusPublished - 1996

    Fingerprint

    Sugar beets
    Beta vulgaris
    Herbicides
    Polyethylene glycols
    DNA
    Genes
    Protoplasts
    Transgenes
    Population
    bialaphos
    phosphinothricin
    Plant Breeding

    Cite this

    Hall, R.D. ; Riksen-Bruinsma, T. ; Weyens, G. ; Rosquin, I.J. ; Denys, R.N. ; Evans, I.J. ; Lathouwers, J.E. ; LefObvre, M.P. ; Dunwell, J.M. ; van Tunen, A. ; Krens, F.A. / A high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells. In: Nature Biotechnology. 1996 ; Vol. 14, No. 9. pp. 1133-1138.
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    title = "A high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells",
    abstract = "An optimized protocol has been developed for the efficient and rapid genetic modification of sugar beet (Beta vulgaris L). A polyethylene glycol- mediated DNA transformation technique could be applied to protoplast populations enriched specifically for a single totipotent cell type derived from stomatal guard cells, to achieve high transformation frequencies. Bialaphos resistance, conferred by the pat gene, produced a highly efficient selection system. The majority of plants were obtained within 8 to 9 weeks and were appropriate for plant breeding purposes. All were resistant to glufosinate-ammonium-based herbicides. Detailed genomic characterization has verified transgene integration, and progeny analysis showed Mendelian inheritance.",
    author = "R.D. Hall and T. Riksen-Bruinsma and G. Weyens and I.J. Rosquin and R.N. Denys and I.J. Evans and J.E. Lathouwers and M.P. LefObvre and J.M. Dunwell and {van Tunen}, A. and F.A. Krens",
    year = "1996",
    doi = "10.1038/nbt0996-1133",
    language = "English",
    volume = "14",
    pages = "1133--1138",
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    Hall, RD, Riksen-Bruinsma, T, Weyens, G, Rosquin, IJ, Denys, RN, Evans, IJ, Lathouwers, JE, LefObvre, MP, Dunwell, JM, van Tunen, A & Krens, FA 1996, 'A high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells', Nature Biotechnology, vol. 14, no. 9, pp. 1133-1138. https://doi.org/10.1038/nbt0996-1133

    A high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells. / Hall, R.D.; Riksen-Bruinsma, T.; Weyens, G.; Rosquin, I.J.; Denys, R.N.; Evans, I.J.; Lathouwers, J.E.; LefObvre, M.P.; Dunwell, J.M.; van Tunen, A.; Krens, F.A.

    In: Nature Biotechnology, Vol. 14, No. 9, 1996, p. 1133-1138.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - A high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells

    AU - Hall, R.D.

    AU - Riksen-Bruinsma, T.

    AU - Weyens, G.

    AU - Rosquin, I.J.

    AU - Denys, R.N.

    AU - Evans, I.J.

    AU - Lathouwers, J.E.

    AU - LefObvre, M.P.

    AU - Dunwell, J.M.

    AU - van Tunen, A.

    AU - Krens, F.A.

    PY - 1996

    Y1 - 1996

    N2 - An optimized protocol has been developed for the efficient and rapid genetic modification of sugar beet (Beta vulgaris L). A polyethylene glycol- mediated DNA transformation technique could be applied to protoplast populations enriched specifically for a single totipotent cell type derived from stomatal guard cells, to achieve high transformation frequencies. Bialaphos resistance, conferred by the pat gene, produced a highly efficient selection system. The majority of plants were obtained within 8 to 9 weeks and were appropriate for plant breeding purposes. All were resistant to glufosinate-ammonium-based herbicides. Detailed genomic characterization has verified transgene integration, and progeny analysis showed Mendelian inheritance.

    AB - An optimized protocol has been developed for the efficient and rapid genetic modification of sugar beet (Beta vulgaris L). A polyethylene glycol- mediated DNA transformation technique could be applied to protoplast populations enriched specifically for a single totipotent cell type derived from stomatal guard cells, to achieve high transformation frequencies. Bialaphos resistance, conferred by the pat gene, produced a highly efficient selection system. The majority of plants were obtained within 8 to 9 weeks and were appropriate for plant breeding purposes. All were resistant to glufosinate-ammonium-based herbicides. Detailed genomic characterization has verified transgene integration, and progeny analysis showed Mendelian inheritance.

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    DO - 10.1038/nbt0996-1133

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