A genome-wide genetic map of NB-LRR disease resistance loci in potato

E.H. Bakker, T.J.A. Borm, J.C.P. Prins, E.A.G. van der Vossen, G.E. Uenk, M.J.B. Arens, J.M. de Boer, H.J. van Eck, M. Muskens, J. Vossen, C.G. van der Linden, R. van der Ham, R.M. Klein Lankhorst, R.G.F. Visser, J. Bakker, A. Goverse

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Like all plants, potato has evolved a surveillance system consisting of a large array of genes encoding for immune receptors that confer resistance to pathogens and pests. The majority of these so-called resistance or R proteins belong to the super-family that harbour a nucleotide binding and a leucine-rich-repeat domain (NB-LRR). Here, sequence information of the conserved NB domain was used to investigate the genome-wide genetic distribution of the NB-LRR resistance gene loci in potato. We analysed the sequences of 288 unique BAC clones selected using filter hybridisation screening of a BAC library of the diploid potato clone RH89-039-16 (S. tuberosum ssp. tuberosum) and a physical map of this BAC library. This resulted in the identification of 738 partial and full-length NB-LRR sequences. Based on homology of these sequences with known resistance genes, 280 and 448 sequences were classified as TIR-NB-LRR (TNL) and CC-NB-LRR (CNL) sequences, respectively. Genetic mapping revealed the presence of 15 TNL and 32 CNL loci. Thirty-six are novel, while three TNL loci and eight CNL loci are syntenic with previously identified functional resistance genes. The genetic map was complemented with 68 universal CAPS markers and 82 disease resistance trait loci described in literature, providing an excellent template for genetic studies and applied research in potato
Original languageEnglish
Pages (from-to)493-508
JournalTheoretical and Applied Genetics
Volume123
Issue number2
DOIs
Publication statusPublished - 2011

Fingerprint

Disease Resistance
Solanum tuberosum
Leucine
leucine
disease resistance
Nucleotides
nucleotides
potatoes
Genome
loci
genome
Genetic Templates
Genes
Libraries
genes
Clone Cells
clones
physical chromosome mapping
Conserved Sequence
Sequence Homology

Keywords

  • nucleotide-binding site
  • downy mildew resistance
  • late blight resistance
  • rich repeat class
  • race-specific resistance
  • flax rust resistance
  • nematode globodera-rostochiensis
  • tuberosum ssp andigena
  • tobacco-mosaic-virus
  • triticum-aestivum l.

Cite this

Bakker, E.H. ; Borm, T.J.A. ; Prins, J.C.P. ; van der Vossen, E.A.G. ; Uenk, G.E. ; Arens, M.J.B. ; de Boer, J.M. ; van Eck, H.J. ; Muskens, M. ; Vossen, J. ; van der Linden, C.G. ; van der Ham, R. ; Klein Lankhorst, R.M. ; Visser, R.G.F. ; Bakker, J. ; Goverse, A. / A genome-wide genetic map of NB-LRR disease resistance loci in potato. In: Theoretical and Applied Genetics. 2011 ; Vol. 123, No. 2. pp. 493-508.
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keywords = "nucleotide-binding site, downy mildew resistance, late blight resistance, rich repeat class, race-specific resistance, flax rust resistance, nematode globodera-rostochiensis, tuberosum ssp andigena, tobacco-mosaic-virus, triticum-aestivum l.",
author = "E.H. Bakker and T.J.A. Borm and J.C.P. Prins and {van der Vossen}, E.A.G. and G.E. Uenk and M.J.B. Arens and {de Boer}, J.M. and {van Eck}, H.J. and M. Muskens and J. Vossen and {van der Linden}, C.G. and {van der Ham}, R. and {Klein Lankhorst}, R.M. and R.G.F. Visser and J. Bakker and A. Goverse",
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A genome-wide genetic map of NB-LRR disease resistance loci in potato. / Bakker, E.H.; Borm, T.J.A.; Prins, J.C.P.; van der Vossen, E.A.G.; Uenk, G.E.; Arens, M.J.B.; de Boer, J.M.; van Eck, H.J.; Muskens, M.; Vossen, J.; van der Linden, C.G.; van der Ham, R.; Klein Lankhorst, R.M.; Visser, R.G.F.; Bakker, J.; Goverse, A.

In: Theoretical and Applied Genetics, Vol. 123, No. 2, 2011, p. 493-508.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - A genome-wide genetic map of NB-LRR disease resistance loci in potato

AU - Bakker, E.H.

AU - Borm, T.J.A.

AU - Prins, J.C.P.

AU - van der Vossen, E.A.G.

AU - Uenk, G.E.

AU - Arens, M.J.B.

AU - de Boer, J.M.

AU - van Eck, H.J.

AU - Muskens, M.

AU - Vossen, J.

AU - van der Linden, C.G.

AU - van der Ham, R.

AU - Klein Lankhorst, R.M.

AU - Visser, R.G.F.

AU - Bakker, J.

AU - Goverse, A.

PY - 2011

Y1 - 2011

N2 - Like all plants, potato has evolved a surveillance system consisting of a large array of genes encoding for immune receptors that confer resistance to pathogens and pests. The majority of these so-called resistance or R proteins belong to the super-family that harbour a nucleotide binding and a leucine-rich-repeat domain (NB-LRR). Here, sequence information of the conserved NB domain was used to investigate the genome-wide genetic distribution of the NB-LRR resistance gene loci in potato. We analysed the sequences of 288 unique BAC clones selected using filter hybridisation screening of a BAC library of the diploid potato clone RH89-039-16 (S. tuberosum ssp. tuberosum) and a physical map of this BAC library. This resulted in the identification of 738 partial and full-length NB-LRR sequences. Based on homology of these sequences with known resistance genes, 280 and 448 sequences were classified as TIR-NB-LRR (TNL) and CC-NB-LRR (CNL) sequences, respectively. Genetic mapping revealed the presence of 15 TNL and 32 CNL loci. Thirty-six are novel, while three TNL loci and eight CNL loci are syntenic with previously identified functional resistance genes. The genetic map was complemented with 68 universal CAPS markers and 82 disease resistance trait loci described in literature, providing an excellent template for genetic studies and applied research in potato

AB - Like all plants, potato has evolved a surveillance system consisting of a large array of genes encoding for immune receptors that confer resistance to pathogens and pests. The majority of these so-called resistance or R proteins belong to the super-family that harbour a nucleotide binding and a leucine-rich-repeat domain (NB-LRR). Here, sequence information of the conserved NB domain was used to investigate the genome-wide genetic distribution of the NB-LRR resistance gene loci in potato. We analysed the sequences of 288 unique BAC clones selected using filter hybridisation screening of a BAC library of the diploid potato clone RH89-039-16 (S. tuberosum ssp. tuberosum) and a physical map of this BAC library. This resulted in the identification of 738 partial and full-length NB-LRR sequences. Based on homology of these sequences with known resistance genes, 280 and 448 sequences were classified as TIR-NB-LRR (TNL) and CC-NB-LRR (CNL) sequences, respectively. Genetic mapping revealed the presence of 15 TNL and 32 CNL loci. Thirty-six are novel, while three TNL loci and eight CNL loci are syntenic with previously identified functional resistance genes. The genetic map was complemented with 68 universal CAPS markers and 82 disease resistance trait loci described in literature, providing an excellent template for genetic studies and applied research in potato

KW - nucleotide-binding site

KW - downy mildew resistance

KW - late blight resistance

KW - rich repeat class

KW - race-specific resistance

KW - flax rust resistance

KW - nematode globodera-rostochiensis

KW - tuberosum ssp andigena

KW - tobacco-mosaic-virus

KW - triticum-aestivum l.

U2 - 10.1007/s00122-011-1602-z

DO - 10.1007/s00122-011-1602-z

M3 - Article

VL - 123

SP - 493

EP - 508

JO - Theoretical and Applied Genetics

JF - Theoretical and Applied Genetics

SN - 0040-5752

IS - 2

ER -