A genetic map of potato : construction and applications

J.M.E. Jacobs

Research output: Thesisexternal PhD, WU

Abstract

<p>This thesis describes the construction of an integrated genetic map of potato <em>(Solanum tuberosum</em> L.) using molecular, morphological and isozyme markers. A general method for map construction, different from previous methods employed in non-inbred plants, is described using JoinMap 1.4. This basic map was used for various applications in potato genetics. For the purpose of targeted transposon tagging in potato, the integration sites of T-DNA's and transposable elements were determined and were found to cover the entire potato genome. Genetic maps, constructed from seven different potato populations, were compared and aligned. All data were combined and one common core map was constructed with the help of JoinMap 2.0. Furthermore, the genetic map was used to assist in the mapping of the resistance locus <em>GroV1</em> from <em>S</em> . <em>vernei.</em> This resistance locus, to the potato cyst nematode <em>Globodera rostochiensis</em> pathotype 1, was located on chromosome <em>5</em> with molecular (RFU/PCR) markers.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
Supervisors/Advisors
  • Jacobsen, Evert, Promotor
  • Stiekema, W.J., Co-promotor, External person
Award date30 Oct 1995
Place of PublicationS.l.
Publisher
Print ISBNs9789054854401
Publication statusPublished - 1995

Keywords

  • solanum tuberosum
  • potatoes
  • chromosome maps
  • gene mapping
  • molecular genetics
  • plant breeding
  • disease resistance
  • pest resistance

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