Abstract
This thesis describes the construction of an integrated genetic map of potato (Solanum tuberosum L.) using molecular, morphological and isozyme markers. A general method for map construction, different from previous methods employed in non-inbred plants, is described using JoinMap 1.4. This basic map was used for various applications in potato genetics. For the purpose of targeted transposon tagging in potato, the integration sites of T-DNA's and transposable elements were determined and were found to cover the entire potato genome. Genetic maps, constructed from seven different potato populations, were compared and aligned. All data were combined and one common core map was constructed with the help of JoinMap 2.0. Furthermore, the genetic map was used to assist in the mapping of the resistance locus GroV1 from S . vernei. This resistance locus, to the potato cyst nematode Globodera rostochiensis pathotype 1, was located on chromosome 5 with molecular (RFU/PCR) markers.
| Original language | English |
|---|---|
| Qualification | Doctor of Philosophy |
| Awarding Institution | |
| Supervisors/Advisors |
|
| Award date | 30 Oct 1995 |
| Place of Publication | Wageningen |
| Publisher | |
| Print ISBNs | 9789054854401 |
| DOIs | |
| Publication status | Published - 30 Oct 1995 |
Keywords
- solanum tuberosum
- potatoes
- chromosome maps
- gene mapping
- molecular genetics
- plant breeding
- disease resistance
- pest resistance