A generic (RT)-PCR test for caulimoviruses

    Research output: Chapter in Book/Report/Conference proceedingAbstract

    Abstract

    For a considerable number of viruses from different genera sequence data are available and for different virus genera general PCR primersets have been described. Not for all economically important viruses reliable detection methods are available yet. Caulimoviruses constitute a group of DNA plant viruses that cause diseases in a wide range of crops. To only a limited number of them specific diagnostics are available. Detection of poorly characterized or new caulimoviruses is often not possible or reliable. Based on available sequence data caulimovirus genus–specific primers were designed The primers were tested to available caulimoviruses from different crops. At least one generic primer set proofed capable of detection of all caulimovirus tested to date. Sequence analysis of the PCR products enabled subsequent identification of the individual viruses. Use of these primer sets will allow NPPOs and inspection services to more reliable monitor caulimovirus infections and their spread.
    Original languageEnglish
    Title of host publicationBook of Abstracts. The 3rd Conference of the International Working Group on Legume and Vegetable viruses (IWGLVV), Ljubljana, Slovenia, 20-23 August 2008
    EditorsJ.Th.J. Verhoeven, J.H. Vetten, M. Krajacic, M. Ravnikar
    Place of PublicationLjubljana, Slovenia
    PublisherNational Institute of Biology (NIB), Department of Biotechnology and Systems Biology
    Pages70 (P18)
    Publication statusPublished - 2008
    EventThe 3rd Conference of the International Working Group on Legume and Vegetable viruses (IWGLVV) - Ljubljana
    Duration: 20 Aug 200823 Aug 2008

    Conference

    ConferenceThe 3rd Conference of the International Working Group on Legume and Vegetable viruses (IWGLVV)
    CityLjubljana
    Period20/08/0823/08/08

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