A gapless genome sequence of the fungus Botrytis cinerea

Jan A.L. Van Kan*, Joost H.M. Stassen, Andreas Mosbach, Theo A.J. Van Der Lee, Luigi Faino, Andrew D. Farmer, Dimitrios G. Papasotiriou, Shiguo Zhou, Michael F. Seidl, Eleanor Cottam, Dominique Edel, Matthias Hahn, David C. Schwartz, Robert A. Dietrich, Stephanie Widdison, Gabriel Scalliet

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

91 Citations (Scopus)

Abstract

Following earlier incomplete and fragmented versions of a genome sequence for the grey mould Botrytis cinerea, a gapless, near-finished genome sequence for B. cinerea strain B05.10 is reported. The assembly comprised 18 chromosomes and was confirmed by an optical map and a genetic map based on approximately 75 000 single nucleotide polymorphism (SNP) markers. All chromosomes contained fully assembled centromeric regions, and 10 chromosomes had telomeres on both ends. The genetic map consisted of 4153 cM and a comparison of the genetic distances with the physical distances identified 40 recombination hotspots. The linkage map also identified two mutations, located in the previously described genes Bos1 and BcsdhB, that conferred resistance to the fungicides boscalid and iprodione. The genome was predicted to encode 11 701 proteins. RNAseq data from >20 different samples were used to validate and improve gene models. Manual curation of chromosome 1 revealed interesting features, such as the occurrence of a dicistronic transcript and fully overlapping genes in opposite orientations, as well as many spliced antisense transcripts. Manual curation also revealed that the untranslated regions (UTRs) of genes can be complex and long, with many UTRs exceeding lengths of 1 kb and possessing multiple introns. Community annotation is in progress.

Original languageEnglish
Pages (from-to)75-89
JournalMolecular Plant Pathology
Volume18
Issue number1
DOIs
Publication statusPublished - 2017

Keywords

  • Genetic map
  • Grey mould
  • Optical map
  • SMRT sequencing

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