A functional F analogue of AcMNPV GP64 is from the Agrotis segetum granulovirus

F. Yin, M. Wang, Y. Tan, F. Deng, J.M. Vlak, Z.H. Hu, H. Wang

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)

Abstract

The envelope fusion protein F of Plutella xylostella granulovirus is a computational analogue of the GP64 envelope fusion protein of Autographa californica nucleopolyhedrovirus (AcMNPV). Granulovirus (GV) F proteins were thought to be unable to functionally replace GP64 in the AcMNPV pseudotyping system. In the present study the F protein of Agrotis segetum GV (AgseGV) was identified experimentally as the first functional GP64 analogue from GVs. AgseF can rescue virion propagation and infectivity of gp64-null AcMNPV. The AgseF-pseudotyped AcMNPV also induced syncytium formation as a consequence of low-pH-induced membrane fusion.
Original languageEnglish
Pages (from-to)8922-8926
JournalJournal of Virology
Volume82
Issue number17
DOIs
Publication statusPublished - 2008

Keywords

  • envelope fusion protein
  • baculovirus gp64
  • multicapsid nucleopolyhedrovirus
  • genome
  • sequence
  • site

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