A European inter-laboratory trial to evaluate the performance of three serological methods for diagnosis of Mycoplasma bovis infection in cattle using latent class analysis

Anna Maria Andersson, Anna Aspán, Henk J. Wisselink*, Bregtje Smid, Anne Ridley, Sinikka Pelkonen, Tiina Autio, Klara Tølbøll Lauritsen, Jane Kensø, Patrice Gaurivaud, Florence Tardy

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

BACKGROUND: Mycoplasma bovis (M. bovis) is an emerging bovine pathogen, leading to significant economic losses in the livestock industry worldwide. Infection can result in a variety of clinical signs, such as arthritis, pneumonia, mastitis and keratoconjunctivitis, none of which are M. bovis-specific. Laboratory diagnosis is therefore important. Serological tests to detect M. bovis antibodies is considered an effective indicator of infection in a herd and often used as a herd test. Combined with clinical judgement, it can also be used to implement control strategies and/or to estimate the disease prevalence within a country. However, due to lack of harmonisation of approaches to testing, and serological tests used by different laboratories, comparisons of prevalence data between countries is often difficult. A network of researchers from six European countries designed and participated in an inter-laboratory trial, with the aim of evaluating the sensitivity (Se) and specificity (Sp) of two commercially available ELISA tests (ID Screen® ELISA (IDvet) and BIO K302 ELISA (BIO-X Diagnostics)) for diagnosis of M. bovis infection. Each laboratory received a blinded panel of bovine sera and tested independently, according to manufacturer's instructions. Western blot analyses (WB) performed by one of the participating laboratories was used as a third diagnostic test in the statistical evaluation of Se and Sp values using latent class analysis. RESULTS: The Se of WB, the ID Screen® ELISA and the BIO K302 ELISA were determined to be 91.8, 93.5 and 49.1% respectively, and corresponding Sp of the three tests were 99.6, 98.6 and 89.6%, respectively. CONCLUSIONS: The present study is, to our knowledge, the first to present an inter-laboratory comparison of the BIO K302 ELISA and the ID Screen® ELISA. Based on our results, the ID Screen® ELISA showed high consistency with WB and performed with higher precision and accuracy than the BIO K302 ELISA.

Original languageEnglish
Article number369
Number of pages1
JournalBMC Veterinary Research
Volume15
Issue number1
DOIs
Publication statusPublished - 25 Oct 2019

Fingerprint

Mycoplasma bovis
Mycoplasma Infections
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
cattle
infection
Western blotting
Western Blotting
methodology
Serologic Tests
immunologic techniques
herds
testing
Keratoconjunctivitis
Sensitivity and Specificity
keratoconjunctivitis
Mastitis
Clinical Laboratory Techniques
livestock and meat industry
disease prevalence

Keywords

  • ELISA
  • Inter-laboratory trial
  • Latent class analysis
  • Mycoplasma bovis cattle
  • Western blot

Cite this

Andersson, Anna Maria ; Aspán, Anna ; Wisselink, Henk J. ; Smid, Bregtje ; Ridley, Anne ; Pelkonen, Sinikka ; Autio, Tiina ; Lauritsen, Klara Tølbøll ; Kensø, Jane ; Gaurivaud, Patrice ; Tardy, Florence. / A European inter-laboratory trial to evaluate the performance of three serological methods for diagnosis of Mycoplasma bovis infection in cattle using latent class analysis. In: BMC Veterinary Research. 2019 ; Vol. 15, No. 1.
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abstract = "BACKGROUND: Mycoplasma bovis (M. bovis) is an emerging bovine pathogen, leading to significant economic losses in the livestock industry worldwide. Infection can result in a variety of clinical signs, such as arthritis, pneumonia, mastitis and keratoconjunctivitis, none of which are M. bovis-specific. Laboratory diagnosis is therefore important. Serological tests to detect M. bovis antibodies is considered an effective indicator of infection in a herd and often used as a herd test. Combined with clinical judgement, it can also be used to implement control strategies and/or to estimate the disease prevalence within a country. However, due to lack of harmonisation of approaches to testing, and serological tests used by different laboratories, comparisons of prevalence data between countries is often difficult. A network of researchers from six European countries designed and participated in an inter-laboratory trial, with the aim of evaluating the sensitivity (Se) and specificity (Sp) of two commercially available ELISA tests (ID Screen{\circledR} ELISA (IDvet) and BIO K302 ELISA (BIO-X Diagnostics)) for diagnosis of M. bovis infection. Each laboratory received a blinded panel of bovine sera and tested independently, according to manufacturer's instructions. Western blot analyses (WB) performed by one of the participating laboratories was used as a third diagnostic test in the statistical evaluation of Se and Sp values using latent class analysis. RESULTS: The Se of WB, the ID Screen{\circledR} ELISA and the BIO K302 ELISA were determined to be 91.8, 93.5 and 49.1{\%} respectively, and corresponding Sp of the three tests were 99.6, 98.6 and 89.6{\%}, respectively. CONCLUSIONS: The present study is, to our knowledge, the first to present an inter-laboratory comparison of the BIO K302 ELISA and the ID Screen{\circledR} ELISA. Based on our results, the ID Screen{\circledR} ELISA showed high consistency with WB and performed with higher precision and accuracy than the BIO K302 ELISA.",
keywords = "ELISA, Inter-laboratory trial, Latent class analysis, Mycoplasma bovis cattle, Western blot",
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A European inter-laboratory trial to evaluate the performance of three serological methods for diagnosis of Mycoplasma bovis infection in cattle using latent class analysis. / Andersson, Anna Maria; Aspán, Anna; Wisselink, Henk J.; Smid, Bregtje; Ridley, Anne; Pelkonen, Sinikka; Autio, Tiina; Lauritsen, Klara Tølbøll; Kensø, Jane; Gaurivaud, Patrice; Tardy, Florence.

In: BMC Veterinary Research, Vol. 15, No. 1, 369, 25.10.2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - A European inter-laboratory trial to evaluate the performance of three serological methods for diagnosis of Mycoplasma bovis infection in cattle using latent class analysis

AU - Andersson, Anna Maria

AU - Aspán, Anna

AU - Wisselink, Henk J.

AU - Smid, Bregtje

AU - Ridley, Anne

AU - Pelkonen, Sinikka

AU - Autio, Tiina

AU - Lauritsen, Klara Tølbøll

AU - Kensø, Jane

AU - Gaurivaud, Patrice

AU - Tardy, Florence

PY - 2019/10/25

Y1 - 2019/10/25

N2 - BACKGROUND: Mycoplasma bovis (M. bovis) is an emerging bovine pathogen, leading to significant economic losses in the livestock industry worldwide. Infection can result in a variety of clinical signs, such as arthritis, pneumonia, mastitis and keratoconjunctivitis, none of which are M. bovis-specific. Laboratory diagnosis is therefore important. Serological tests to detect M. bovis antibodies is considered an effective indicator of infection in a herd and often used as a herd test. Combined with clinical judgement, it can also be used to implement control strategies and/or to estimate the disease prevalence within a country. However, due to lack of harmonisation of approaches to testing, and serological tests used by different laboratories, comparisons of prevalence data between countries is often difficult. A network of researchers from six European countries designed and participated in an inter-laboratory trial, with the aim of evaluating the sensitivity (Se) and specificity (Sp) of two commercially available ELISA tests (ID Screen® ELISA (IDvet) and BIO K302 ELISA (BIO-X Diagnostics)) for diagnosis of M. bovis infection. Each laboratory received a blinded panel of bovine sera and tested independently, according to manufacturer's instructions. Western blot analyses (WB) performed by one of the participating laboratories was used as a third diagnostic test in the statistical evaluation of Se and Sp values using latent class analysis. RESULTS: The Se of WB, the ID Screen® ELISA and the BIO K302 ELISA were determined to be 91.8, 93.5 and 49.1% respectively, and corresponding Sp of the three tests were 99.6, 98.6 and 89.6%, respectively. CONCLUSIONS: The present study is, to our knowledge, the first to present an inter-laboratory comparison of the BIO K302 ELISA and the ID Screen® ELISA. Based on our results, the ID Screen® ELISA showed high consistency with WB and performed with higher precision and accuracy than the BIO K302 ELISA.

AB - BACKGROUND: Mycoplasma bovis (M. bovis) is an emerging bovine pathogen, leading to significant economic losses in the livestock industry worldwide. Infection can result in a variety of clinical signs, such as arthritis, pneumonia, mastitis and keratoconjunctivitis, none of which are M. bovis-specific. Laboratory diagnosis is therefore important. Serological tests to detect M. bovis antibodies is considered an effective indicator of infection in a herd and often used as a herd test. Combined with clinical judgement, it can also be used to implement control strategies and/or to estimate the disease prevalence within a country. However, due to lack of harmonisation of approaches to testing, and serological tests used by different laboratories, comparisons of prevalence data between countries is often difficult. A network of researchers from six European countries designed and participated in an inter-laboratory trial, with the aim of evaluating the sensitivity (Se) and specificity (Sp) of two commercially available ELISA tests (ID Screen® ELISA (IDvet) and BIO K302 ELISA (BIO-X Diagnostics)) for diagnosis of M. bovis infection. Each laboratory received a blinded panel of bovine sera and tested independently, according to manufacturer's instructions. Western blot analyses (WB) performed by one of the participating laboratories was used as a third diagnostic test in the statistical evaluation of Se and Sp values using latent class analysis. RESULTS: The Se of WB, the ID Screen® ELISA and the BIO K302 ELISA were determined to be 91.8, 93.5 and 49.1% respectively, and corresponding Sp of the three tests were 99.6, 98.6 and 89.6%, respectively. CONCLUSIONS: The present study is, to our knowledge, the first to present an inter-laboratory comparison of the BIO K302 ELISA and the ID Screen® ELISA. Based on our results, the ID Screen® ELISA showed high consistency with WB and performed with higher precision and accuracy than the BIO K302 ELISA.

KW - ELISA

KW - Inter-laboratory trial

KW - Latent class analysis

KW - Mycoplasma bovis cattle

KW - Western blot

U2 - 10.1186/s12917-019-2117-0

DO - 10.1186/s12917-019-2117-0

M3 - Article

VL - 15

JO - BMC Veterinary Research

JF - BMC Veterinary Research

SN - 1746-6148

IS - 1

M1 - 369

ER -