A distinct tospovirus causing necrotic straek on alstroemeria sp. in Colombia

A. Mehraban, M. Botermans, J.Th.J. Verhoeven, E. Meekes, J. Saaijer, D. Peters, R.W. Goldbach, R.J.M. Kormelink

Research output: Contribution to journalArticleAcademicpeer-review

51 Citations (Scopus)

Abstract

A tospovirus causing necrotic streaks on leaves was isolated from Alstroemeria sp. in Colombia. Infected samples reacted positively with tomato spotted wilt virus (TSWV) antiserum during preliminary serological tests. Further analysis revealed a close serological relationship to tomato chlorotic spot virus (TCSV) and groundnut ringspot virus (GRSV). A major part of the S-RNA segment, encompassing the nucleocapsid (N) protein gene, the 5' untranslated region and a part of the intergenic region 3' of the N gene, was cloned and sequenced. The deduced N protein sequence showed highest amino acid identity (82%) to that of TCSV, indicating that the virus represents a new tospovirus species, for which the name Alstroemeria necrotic streak virus (ANSV) is coined. Phylogenetic analysis based on the N protein sequence revealed that this Alstroemeria-infecting tospovirus clustered with tospoviruses from the American continent. Frankliniella occidentalis was identified as potential vector species for ANSV.
Original languageEnglish
Pages (from-to)423-428
JournalArchives of Virology
Volume155
Issue number3
DOIs
Publication statusPublished - 2010

Keywords

  • spotted wilt virus
  • rna segment
  • tomato
  • identification
  • chrysanthemum
  • sequence
  • protein
  • isolate
  • brazil

Fingerprint

Dive into the research topics of 'A distinct tospovirus causing necrotic straek on alstroemeria sp. in Colombia'. Together they form a unique fingerprint.

Cite this