A detailed analysis of the recombination landscape of the button mushroom Agaricus bisporus var. bisporus

Anton S.M. Sonnenberg, Wei Gao, Brian Lavrijssen, Patrick Hendrickx, Narges Sedaghat-Tellgerd, Marie Foulongne-Oriol, Won-Sik Kong, Elio G.W.M. Schijlen, Johan J.P. Baars, Richard G.F. Visser

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)

Abstract

The button mushroom (Agaricus bisporus) is one of the world’s most cultivated mushroom species, but in spite of its economic importance generation of new cultivars by outbreeding is exceptional. Previous genetic analyses of the white bisporus variety, including all cultivars and most wild isolates revealed that crossing over frequencies are low, which might explain the lack of introducing novel traits into existing cultivars. By generating two high quality whole genome sequence assemblies (one de novo and the other by improving the existing reference genome) of the first commercial white hybrid Horst U1, a detailed study of the crossover (CO) landscape was initiated. Using a set of 626 SNPs in a haploid offspring of 139 single spore isolates and whole genome sequencing on a limited number of homo- and heterokaryotic single spore isolates, we precisely mapped all COs showing that they are almost exclusively restricted to regions of about 100 kb at the chromosome ends. Most basidia of A. bisporus var. bisporus produce two spores and pair preferentially via non-sister nuclei. Combined with the COs restricted to the chromosome ends, these spores retain most of the heterozygosity of the parent thus explaining how present-day white cultivars are genetically so close to the first hybrid marketed in 1980. To our knowledge this is the first example of an organism which displays such specific CO landscape.
Original languageEnglish
Pages (from-to)35-45
JournalFungal Genetics and Biology
Volume93
DOIs
Publication statusPublished - 2016

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Agaricus
Agaricales
Spores
Genetic Recombination
Genome
Chromosomes
Haploidy
Cross-Over Studies
Single Nucleotide Polymorphism
Economics

Cite this

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title = "A detailed analysis of the recombination landscape of the button mushroom Agaricus bisporus var. bisporus",
abstract = "The button mushroom (Agaricus bisporus) is one of the world’s most cultivated mushroom species, but in spite of its economic importance generation of new cultivars by outbreeding is exceptional. Previous genetic analyses of the white bisporus variety, including all cultivars and most wild isolates revealed that crossing over frequencies are low, which might explain the lack of introducing novel traits into existing cultivars. By generating two high quality whole genome sequence assemblies (one de novo and the other by improving the existing reference genome) of the first commercial white hybrid Horst U1, a detailed study of the crossover (CO) landscape was initiated. Using a set of 626 SNPs in a haploid offspring of 139 single spore isolates and whole genome sequencing on a limited number of homo- and heterokaryotic single spore isolates, we precisely mapped all COs showing that they are almost exclusively restricted to regions of about 100 kb at the chromosome ends. Most basidia of A. bisporus var. bisporus produce two spores and pair preferentially via non-sister nuclei. Combined with the COs restricted to the chromosome ends, these spores retain most of the heterozygosity of the parent thus explaining how present-day white cultivars are genetically so close to the first hybrid marketed in 1980. To our knowledge this is the first example of an organism which displays such specific CO landscape.",
author = "Sonnenberg, {Anton S.M.} and Wei Gao and Brian Lavrijssen and Patrick Hendrickx and Narges Sedaghat-Tellgerd and Marie Foulongne-Oriol and Won-Sik Kong and Schijlen, {Elio G.W.M.} and Baars, {Johan J.P.} and Visser, {Richard G.F.}",
year = "2016",
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journal = "Fungal Genetics and Biology",
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A detailed analysis of the recombination landscape of the button mushroom Agaricus bisporus var. bisporus. / Sonnenberg, Anton S.M.; Gao, Wei; Lavrijssen, Brian; Hendrickx, Patrick; Sedaghat-Tellgerd, Narges; Foulongne-Oriol, Marie; Kong, Won-Sik; Schijlen, Elio G.W.M.; Baars, Johan J.P.; Visser, Richard G.F.

In: Fungal Genetics and Biology, Vol. 93, 2016, p. 35-45.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - A detailed analysis of the recombination landscape of the button mushroom Agaricus bisporus var. bisporus

AU - Sonnenberg, Anton S.M.

AU - Gao, Wei

AU - Lavrijssen, Brian

AU - Hendrickx, Patrick

AU - Sedaghat-Tellgerd, Narges

AU - Foulongne-Oriol, Marie

AU - Kong, Won-Sik

AU - Schijlen, Elio G.W.M.

AU - Baars, Johan J.P.

AU - Visser, Richard G.F.

PY - 2016

Y1 - 2016

N2 - The button mushroom (Agaricus bisporus) is one of the world’s most cultivated mushroom species, but in spite of its economic importance generation of new cultivars by outbreeding is exceptional. Previous genetic analyses of the white bisporus variety, including all cultivars and most wild isolates revealed that crossing over frequencies are low, which might explain the lack of introducing novel traits into existing cultivars. By generating two high quality whole genome sequence assemblies (one de novo and the other by improving the existing reference genome) of the first commercial white hybrid Horst U1, a detailed study of the crossover (CO) landscape was initiated. Using a set of 626 SNPs in a haploid offspring of 139 single spore isolates and whole genome sequencing on a limited number of homo- and heterokaryotic single spore isolates, we precisely mapped all COs showing that they are almost exclusively restricted to regions of about 100 kb at the chromosome ends. Most basidia of A. bisporus var. bisporus produce two spores and pair preferentially via non-sister nuclei. Combined with the COs restricted to the chromosome ends, these spores retain most of the heterozygosity of the parent thus explaining how present-day white cultivars are genetically so close to the first hybrid marketed in 1980. To our knowledge this is the first example of an organism which displays such specific CO landscape.

AB - The button mushroom (Agaricus bisporus) is one of the world’s most cultivated mushroom species, but in spite of its economic importance generation of new cultivars by outbreeding is exceptional. Previous genetic analyses of the white bisporus variety, including all cultivars and most wild isolates revealed that crossing over frequencies are low, which might explain the lack of introducing novel traits into existing cultivars. By generating two high quality whole genome sequence assemblies (one de novo and the other by improving the existing reference genome) of the first commercial white hybrid Horst U1, a detailed study of the crossover (CO) landscape was initiated. Using a set of 626 SNPs in a haploid offspring of 139 single spore isolates and whole genome sequencing on a limited number of homo- and heterokaryotic single spore isolates, we precisely mapped all COs showing that they are almost exclusively restricted to regions of about 100 kb at the chromosome ends. Most basidia of A. bisporus var. bisporus produce two spores and pair preferentially via non-sister nuclei. Combined with the COs restricted to the chromosome ends, these spores retain most of the heterozygosity of the parent thus explaining how present-day white cultivars are genetically so close to the first hybrid marketed in 1980. To our knowledge this is the first example of an organism which displays such specific CO landscape.

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VL - 93

SP - 35

EP - 45

JO - Fungal Genetics and Biology

JF - Fungal Genetics and Biology

SN - 1087-1845

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